Pipette ndi chimodzi mwa zida zomwe zimagwiritsidwa ntchito m'ma laboratories a zamoyo, zachipatala, ndi zowunikira momwe zamadzimadzi zimafunikira kuyesedwa ndendende ndikusamutsidwa popanga dilution, kuyesa kapena kuyesa magazi. Amapezeka ngati:
① njira imodzi kapena zingapo
② voliyumu yokhazikika kapena yosinthika
③ pamanja kapena zamagetsi
Kodi Single-Channel Pipettes ndi chiyani?
Pipette yokhala ndi njira imodzi imalola ogwiritsa ntchito kusamutsa aliquot imodzi panthawi imodzi. Izi zimakonda kugwiritsidwa ntchito m'ma laboratories okhala ndi zitsanzo zochepa, zomwe nthawi zambiri zimakhala zomwe zimakhudzidwa ndi kafukufuku ndi chitukuko.
Pipette yokhala ndi njira imodzi imakhala ndi mutu umodzi wofuna kutulutsa kapena kutulutsa madzi olondola kwambiri kudzera muzotayira.nsonga. Atha kugwiritsidwa ntchito pazinthu zingapo m'ma laboratories omwe amangotulutsa pang'ono. Awa nthawi zambiri amakhala ma laboratories omwe amachita kafukufuku wokhudzana ndi chemistry yowunikira, chikhalidwe cha ma cell, genetics kapena immunology.
Kodi Multi-Channel Pipettes ndi chiyani?
Ma pipette a njira zambiri amagwira ntchito mofanana ndi mapaipi amtundu umodzi, koma amagwiritsa ntchito angapomalangizokuyeza ndi kugawira madzi amadzimadzi ofanana nthawi imodzi. Kukhazikitsa wamba ndi njira 8 kapena 12 koma ma seti 4, 6, 16 ndi 48 akupezekanso. Mitundu ya benchtop ya 96 ingagulidwenso.
Pogwiritsa ntchito pipette yamitundu yambiri, ndikosavuta kudzaza chitsime cha 96-, 384-, kapena 1,536mbale ya microtiter, yomwe ingakhale ndi zitsanzo za ntchito monga DNA amplification, ELISA (diagnostic test), maphunziro a kinetic ndi kufufuza kwa maselo.
Single-Channel vs. Multi-Channel Pipettes
Kuchita bwino
Pipette ya njira imodzi ndi yabwino pochita ntchito yoyesera. Izi zili choncho chifukwa zimangogwiritsa ntchito machubu amodzi, kapena njira imodzi yolumikizirana poika magazi.
Komabe, izi zimangokhala chida chosagwira ntchito pamene kutulutsa kumawonjezeka. Pakakhala zitsanzo / zopangira zingapo zosamutsa, kapena zoyeserera zazikulu zikuyendetsedwa96 bwino microtitre mbale, pali njira yabwino kwambiri yosamutsa zakumwa kenako pogwiritsa ntchito pipette imodzi. Pogwiritsa ntchito pipette yamitundu yambiri m'malo mwake, chiwerengero cha masitepe a pipetting chimachepetsedwa kwambiri.
Gome ili m'munsili likuwonetsa kuchuluka kwa mapaipi ofunikira pakukhazikitsa njira imodzi, 8 ndi 12.
Chiwerengero cha mapaipi ofunikira (6 reagents x96 Well Microtitre Plate)
Njira imodzi Pipette: 576
8-Channel Pipette: 72
12-Channel Pipette: 48
Kuchuluka kwa Pipetting
Kusiyana kwakukulu pakati pa pipettes imodzi ndi njira zambiri ndi voliyumu pachitsime chomwe chingasamutsidwe nthawi imodzi. Ngakhale zimatengera mtundu womwe ukugwiritsidwa ntchito, nthawi zambiri simungathe kusamutsa voliyumu yochuluka pamutu pa pipette yamitundu yambiri.
Voliyumu yomwe pipette yamtundu umodzi imatha kusamutsa pakati pa 0.1ul ndi 10,000ul, pomwe mitundu yambiri ya pipette imakhala pakati pa 0.2 ndi 1200ul.
Zitsanzo Loading
M'mbuyomu, ma pipette amakanema ambiri akhala osagwira ntchito komanso ovuta kugwiritsa ntchito. Izi zapangitsa kutsitsa kwachitsanzo mosagwirizana, limodzi ndi zovuta pakutsitsamalangizo. Pali mitundu yatsopano yomwe ilipo tsopano, yomwe ndi yosavuta kugwiritsa ntchito ndipo imapita njira ina yothetsera vutoli. Ndizofunikiranso kudziwa kuti ngakhale kutsitsa kwamadzimadzi kungakhale kolakwika pang'ono ndi pipette yamitundu yambiri, kumakhala kolondola kwambiri kuposa njira imodzi chifukwa cha zolakwika zomwe zimachitika chifukwa cha zolakwika za ogwiritsa ntchito chifukwa cha kutopa ( onani ndime yotsatira).
Kuchepetsa Zolakwa za Anthu
Kuthekera kwa cholakwika chamunthu kumachepetsedwa kwambiri pomwe kuchuluka kwa maipiipi kumachepa. Kusiyanasiyana kwa kutopa ndi kutopa kumachotsedwa, zomwe zimapangitsa kuti deta ndi zotsatira zake zikhale zodalirika komanso zowonjezereka.
Kuwongolera
Kuti muwonetsetse kuti zida zogwirira ntchito zamadzimadzi zimakhala zolondola komanso zolondola, kuwongolera pafupipafupi kumafunika. Muyeso wa ISO 8655 umanena kuti njira iliyonse iyenera kuyesedwa ndikufotokozedwa. Pamene pipette ili ndi njira zambiri, zimatengera nthawi kuti ziwongolere zomwe zingakhale zowononga nthawi.
Malinga ndi pipettecalibration.net kusinthasintha kwa 2.2 pa pipette ya 12-channel kumafuna mizere 48 ya pipetting ndi zolemera za gravimetric (2 voliyumu × 2 kubwereza x 12 njira). Kutengera liwiro la woyendetsa, izi zitha kutenga maola opitilira 1.5 pa pipette. Ma laboratories ku United Kingdom omwe amafunikira kuwerengetsa kwa UKAS angafunike kuchita ma sikelo a 360 gravimetric (ma voliyumu 3 x 10 kubwereza x 12 njira). Kuyesa kuchuluka kwa mayesowa pamanja kumakhala kosatheka ndipo kumatha kupitilira nthawi yomwe yasungidwa pogwiritsa ntchito pipette yanjira zambiri m'ma laboratories ena.
Komabe, kuti athetse mavutowa ma pipette calibration services akupezeka ku makampani angapo. Zitsanzo za izi ndi Gilson Labs, ThermoFisher ndi Pipette Lab.
Kukonza
Sichinthu chimene ambiri amaganiza pogula pipette yatsopano, koma zobwezeredwa za pipettes zamitundu yambiri sizingakonzedwe. Izi zikutanthauza kuti ngati tchanelo cha 1 chawonongeka, zochulukitsa zonse zitha kusinthidwa. Komabe, opanga ena amagulitsa zosintha zamayendedwe apawokha, kotero onetsetsani kuti mwayang'ana kukonzanso ndi wopanga pogula pipette yamakina ambiri.
Chidule - Single vs Multi-Channel Pipettes
Pipette yamitundu yambiri ndi chida chamtengo wapatali kwa labotale iliyonse yomwe ili ndi china chilichonse kuposa zitsanzo zazing'ono kwambiri. Pafupifupi muzochitika zilizonse kuchuluka kwamadzimadzi komwe kumafunikira kusamutsidwa kumakhala kokwanira kwa aliyensensongapa pipette yamitundu yambiri, ndipo pali zovuta zochepa zomwe zimagwirizanitsidwa ndi izi. Kuwonjezeka kulikonse kwakung'ono kwa zovuta pakugwiritsa ntchito pipette yamitundu yambiri kumachulukitsidwa kwambiri ndi kuchepa kwa ukonde wa ntchito, zomwe zimathandizidwa ndi kuchepa kwakukulu kwa masitepe a mapaipi. Zonsezi zikutanthauza kutonthoza kwa ogwiritsa ntchito, ndikuchepetsa zolakwika za ogwiritsa ntchito.
Nthawi yotumiza: Dec-16-2022