Chifukwa Chiyani Zogwiritsidwa Ntchito Zama Laboratory Zimafunika Kuti Zikhale DNase ndi RNase Zaulere?

Pankhani ya biology ya mamolekyulu, kulondola ndi kudalirika ndikofunikira kwambiri. Kuipitsidwa kulikonse muzakudya za labotale kumatha kubweretsa zotsatira zolakwika, zomwe zitha kukhala ndi zotsatirapo zoyipa pakufufuza kwasayansi ndi kuwunika. Chinthu chimodzi chodziwika bwino cha kuipitsidwa ndi kupezeka kwa michere ya DNase ndi RNase. Ma enzymes awa amawononga DNA ndi RNA, motsatana, ndipo amapezeka m'matrices osiyanasiyana achilengedwe. Kuchepetsa chiopsezo cha kuipitsidwa ndikuwonetsetsa zotsatira zolondola, zogwiritsidwa ntchito za labotale, mongamalangizo a pipette, mbale zakuya zachitsime, PCR mbale, ndi machubu, ziyenera kukhala DNase ndi RNase zaulere.

Ma enzymes a DNase ndi RNase amapezeka paliponse ndipo amapezeka m'malo osiyanasiyana achilengedwe, kuphatikiza thupi la munthu, zomera, ndi tizilombo tating'onoting'ono. Amagwira ntchito zofunika kwambiri pama cell monga kugawika kwa DNA, kukonza DNA, ndi kuwonongeka kwa RNA. Komabe, kupezeka kwawo mu labotale kumatha kuwononga kuyesa kophatikiza DNA ndi RNA.

Malangizo a Pipette ndi amodzi mwazinthu zomwe zimagwiritsidwa ntchito kwambiri mu labotale. Amagwiritsidwa ntchito poyendetsa bwino komanso molondola zamadzimadzi, zomwe zimawapangitsa kukhala ofunikira pazinthu zosiyanasiyana monga kukonzekera zitsanzo, kutsata kwa DNA, ndi PCR. Ngati nsonga za pipette sizili za DNase ndi RNase zaulere, kuipitsidwa kumatha kuchitika panthawi ya pipetting, zomwe zimayambitsa kuwonongeka kwa zitsanzo za DNA kapena RNA. Izi zingapangitse zotsatira zabodza kapena zosatsimikizika, zomwe zingawononge kukhulupirika kwa kuyesa konse.

Ma mbale a zitsime zakuya ndi labotale ina yofunika kudyedwa, makamaka pakugwiritsa ntchito kwambiri. Amagwiritsidwa ntchito posungirako zitsanzo, ma serial dilutions, ndi chikhalidwe cha cell. Ngati mbalezi zilibe DNase ndi RNase zaulere, zitsanzo zilizonse za DNA kapena RNA zosungidwa mmenemo zimatha kuipitsidwa, zomwe zimapangitsa kuti ma nucleic acid awonongeke. Izi zitha kusokoneza kulondola kwa mapulogalamu otsika monga PCR, qPCR, kapena kutsatizana kwa mibadwo yotsatira.

Mofananamo, mbale za PCR ndi machubu ndizofunikira kwambiri pamapulogalamu a polymerase chain reaction (PCR). PCR ndi njira yomwe imagwiritsidwa ntchito kwambiri pakukulitsa mayendedwe a DNA. Ngati mbale za PCR ndi machubu ali ndi DNase kapena RNase, njira yowonjezeretsa ikhoza kusokonezedwa, zomwe zimapangitsa kuti pakhale zotsatira zolakwika komanso kutanthauzira zabodza. DNase ndi RNase-free PCR consumables amalepheretsa kuwonongeka kwa chandamale cha DNA kapena RNA panthawi yokulitsa, kuwonetsetsa kuti zotsatira zodalirika komanso zobwereketsa.

Pofuna kuthana ndi vuto la kuipitsidwa, zogwiritsidwa ntchito mu labotale ziyenera kupangidwa ndi njira zoyendetsedwa bwino komanso zida zomwe zimatsimikiziridwa kuti ndi DNase ndi RNase zaulere. Makampani monga Suzhou Ace Biomedical Technology Co., Ltd., amakhazikika pakupanga zinthu zomwe zimagwiritsidwa ntchito mu labotale zomwe zimakwaniritsa zofunikira izi. Monga wopanga wamkulu m'munda, Suzhou Ace Biomedical Technology Co., Ltd. imayika patsogolo khalidwe ndi kudalirika.

Suzhou Ace Biomedical Technology Co., Ltd. imamvetsetsa zovuta za kuipitsidwa kwa DNase ndi RNase muzogwiritsidwa ntchito mu labotale. Malangizo awo a pipette, mbale zakuya zachitsime, mbale za PCR, ndi machubu onse amapangidwa pogwiritsa ntchito zipangizo zamtengo wapatali zomwe zimatsata njira zoyendetsera khalidwe kuti zitsimikizire kuti ndi DNase ndi RNase zaulere.

Kampaniyo imagwiritsa ntchito njira zopangira zotsogola ndikutsata miyezo yapamwamba kwambiri kuti ithetse chiwopsezo choipitsidwa, motero imatsimikizira zotsatira zolondola komanso zodalirika kwa ofufuza ndi azachipatala. Amamvetsetsa kuti kulolerana kulikonse pazakudya za labotale kumatha kukhala ndi zotulukapo zazikulu, osati pazofufuza zokha komanso m'machitidwe azachipatala komwe kuwunika kolondola ndikofunikira.

Pomaliza, zogwiritsidwa ntchito mu labotale monga malangizo a pipette, mbale zakuya zachitsime, mbale za PCR, ndi machubu ziyenera kukhala za DNase ndi RNase zaulere kuti zitsimikizire kulondola ndi kudalirika kwa kuyesa kwa biology ya maselo. Kuipitsidwa ndi ma enzyme amenewa kungayambitse kuwonongeka kwa zitsanzo za DNA ndi RNA, kusokoneza kutsimikizika kwa zotsatira zomwe zapezedwa. Makampani ngatiMalingaliro a kampani Suzhou Ace Biomedical Technology Co., Ltd. kumvetsetsa kufunikira kwa kupanga zopangira zomwe zimakwaniritsa zofunikira izi, zomwe zimathandiza asayansi ndi azachipatala kuti azichita ntchito yawo molimba mtima komanso molondola.