Kodi in vitro diagnosis ndi chiyani?

In vitro diagnostics imatanthawuza njira yodziwira matenda kapena mkhalidwe poyika zitsanzo zachilengedwe kuchokera kunja kwa thupi. Izi zimadalira kwambiri njira zosiyanasiyana za biology, kuphatikiza PCR ndi nucleic acid m'zigawo. Kuphatikiza apo, kasamalidwe kamadzimadzi ndi gawo lofunikira pakuwunika kwa in vitro.

PCR kapena polymerase chain reaction ndi njira yomwe imagwiritsidwa ntchito kukulitsa zidutswa za DNA. Pogwiritsa ntchito zoyambira zenizeni, PCR imalola kukulitsa kosankha kwa ma DNA, omwe amatha kuwunikidwa ngati zizindikiro za matenda kapena matenda. PCR nthawi zambiri imagwiritsidwa ntchito pozindikira matenda a virus, mabakiteriya, mafangasi ndi parasitic, komanso matenda amtundu ndi khansa.

Kutulutsa kwa Nucleic acid ndi njira yomwe imagwiritsidwa ntchito popatula ndikuyeretsa DNA kapena RNA kuchokera ku zitsanzo zachilengedwe. Ma nucleic acid omwe amachotsedwa amapezeka kuti afufuzidwenso, kuphatikiza PCR. Kutulutsa kwa nucleic acid ndikofunikira pakuzindikiritsa molondola komanso kukonza chithandizo cha matenda ndi mikhalidwe yosiyanasiyana.

Kusamalira zamadzimadzi ndi njira yomwe imakhudza kusamutsa, kugawira ndi kusakaniza zamadzimadzi pang'ono mu labotale. Makina ogwiritsira ntchito madzi amadzimadzi ayamba kutchuka kwambiri m'zaka zaposachedwa chifukwa amathandizira kupititsa patsogolo komanso kulondola kwambiri pakuyesa monga PCR ndi nucleic acid m'zigawo.

Kuwunika kwa in vitro kumadalira kwambiri njira zamamolekyulu za biology chifukwa zimalola kuzindikira ndi kusanthula zizindikiro zokhudzana ndi matenda okhudzana ndi majini ndi mamolekyulu. Mwachitsanzo, PCR ikhoza kugwiritsidwa ntchito kukulitsa mndandanda wamtundu wina wokhudzana ndi khansa ya m'mawere, pamene nucleic acid extraction ingagwiritsidwe ntchito kupatula DNA yochokera ku chotupa kuchokera ku zitsanzo za magazi.

Kuphatikiza pa njirazi, njira ndi zida zina zosiyanasiyana zimagwiritsidwa ntchito mu in vitro diagnostics. Mwachitsanzo, zida za microfluidic zimagwiritsidwa ntchito mochulukirachulukira komanso kugwiritsa ntchito mfundo zosamalira. Zipangizozi zidapangidwa kuti zizigwira bwino komanso kuwongolera zakumwa zing'onozing'ono, zomwe zimawapangitsa kukhala abwino kwa PCR ndi ntchito zina zama cell biology.

Momwemonso, matekinoloje a m'badwo wotsatira (NGS) akugwiranso ntchito yofunika kwambiri pakuwunika kwa in vitro. NGS imathandizira kutsatizana kofanana kwa zidutswa za DNA mamiliyoni ambiri, zomwe zimathandiza kuzindikira mwachangu komanso molondola za masinthidwe okhudzana ndi matenda. NGS ili ndi kuthekera kosintha kazindikiridwe ndi chithandizo cha matenda amtundu ndi khansa.

Mwachidule, diagnostics mu vitro ndi gawo lofunikira pazamankhwala amakono ndipo amadalira kwambiri njira zama cell biology monga PCR, nucleic acid m'zigawo, komanso kusamalira madzi. Ukadaulo uwu, limodzi ndi umisiri monga zida za microfluidic ndi NGS, zikusintha momwe timadziwira ndikuchizira matenda. Pamene ukadaulo ukupitilirabe patsogolo, zowunikira mu vitro zitha kukhala zolondola komanso zogwira mtima, pamapeto pake kuwongolera zotulukapo za odwala komanso moyo wabwino.

At Suzhou Ace Biomedical,tadzipereka kukupatsirani ma labu apamwamba kwambiri pazosowa zanu zonse zasayansi. Upangiri wathu wamtundu wa pipette, mbale za PCR, machubu a PCR, ndi filimu yosindikiza zidapangidwa mwaluso ndikupangidwa mwaluso kuti zitsimikizire zolondola komanso zolondola pazoyeserera zanu zonse. Malangizo athu a pipette ndi ogwirizana ndi mitundu yonse yayikulu ya pipette ndipo amabwera mosiyanasiyana kuti agwirizane ndi zosowa zanu zenizeni. Ma mbale athu a PCR ndi machubu amapangidwa kuchokera kuzinthu zapamwamba kwambiri ndipo amapangidwa kuti athe kupirira matenthedwe angapo akumatenthedwa ndikusunga kukhulupirika kwachitsanzo. Filimu yathu yosindikiza imapereka chisindikizo cholimba kuti chiteteze kuphulika ndi kuipitsidwa ndi zinthu zakunja. Timamvetsetsa kufunikira kwa ma labu odalirika komanso ogwira mtima, chifukwa chake timayesetsa kukupatsirani zinthu ndi ntchito zabwino kwambiri zomwe tingathe. Gulu lathu la akatswiri limakhalapo nthawi zonse kuti likuthandizeni ndi mafunso kapena nkhawa zomwe mungakhale nazo.