zomwe ziyenera kutsatiridwa pochotsa choleraMalangizo a Pipette? Tiyeni tione limodzi.
1. Yatsani nsonga ndi nyuzipepala
Ikani mu bokosi la nsonga kuti muchepetse kutentha kwa chinyezi, madigiri 121, 1bar mumlengalenga kuthamanga, mphindi 20; kuti mupewe vuto la nthunzi wa madzi, mutha kukulunga bokosi la nsonga ndi nyuzipepala, kapena kuyiyika mu chofungatira mutatha kuthirira kuti muume.
2. Pamene autoclaving, nsonga bokosi ayenera wokutidwa mu nyuzipepala kuti yolera yotseketsa
Zovala zamanyuzipepala zimatha kuyamwa madzi ndipo zimatha kupewa madzi ochulukirapo, chofunikira kwambiri ndikupewa kuipitsidwanso.
3. Zinthu zomwe zimafunikira chisamaliro pakuchotsa nsonga za pipette pa RNA m'zigawo
Gwiritsani ntchito machubu wamba a EP ndi malangizo a pipette. Musanayambe autoclaving, zilowerereni m'madzi a DEPC usiku wonse kuti muchotse RNase. Mukachotsa DEPC tsiku lotsatira, ikani mu bokosi laling'ono la pipette kuti muchepetse kutentha kwa chinyezi. 121 madigiri, 15-20 mphindi. Pofuna kupewa vuto la nthunzi wamadzi, nyuzipepala zimatha kukulunga pabokosi laling'ono, kapena kuziyika mu chofungatira kuti ziume pambuyo potseketsa. Ndi bwino kuti samatenthetsa mwachindunji aliyense m'zigawo, ndipo musagwiritse ntchito nthawi yaitali nsonga pipette kuchotsa RNA.
Ubwino wa sterilization yotentha kwambiri:
Kulowa kwamphamvu kwa kutentha kwa nthunzi; mkulu wotseketsa bwino; nthawi yochepa yolera; palibe mankhwala kapena kuipitsidwa kwa thupi panthawi yotseketsa; zochepa zowongolera zida zotsekereza ndi ntchito yokhazikika; kutsekereza kwa nthunzi kumagwiritsidwa ntchito kusunga madzi ndi mphamvu. Kutentha kwapamwamba kwambiri.
Malangizo a pipette a Yongyue amapangidwa ndi zinthu zachipatala za polypropylene (PP), zomwe zimakumana ndi kalasi ya USP VI, zimakhala bwino kwambiri ndi mankhwala, ndipo zimatha kutsukidwa pa kutentha kwa madigiri 121 ndi kuthamanga kwambiri (mankhwala amtundu wa ma elekitiroti oletsa kulera) .
1. Yatsani nsonga ndi nyuzipepala
Ikani mu bokosi la nsonga kuti muchepetse kutentha kwa chinyezi, madigiri 121, 1bar mumlengalenga kuthamanga, mphindi 20; kuti mupewe vuto la nthunzi wa madzi, mutha kukulunga bokosi la nsonga ndi nyuzipepala, kapena kuyiyika mu chofungatira mutatha kuthirira kuti muume.
2. Pamene autoclaving, nsonga bokosi ayenera wokutidwa mu nyuzipepala kuti yolera yotseketsa
Zovala zamanyuzipepala zimatha kuyamwa madzi ndipo zimatha kupewa madzi ochulukirapo, chofunikira kwambiri ndikupewa kuipitsidwanso.
3. Zinthu zomwe zimafunikira chisamaliro pakuchotsa nsonga za pipette pa RNA m'zigawo
Gwiritsani ntchito machubu wamba a EP ndi malangizo a pipette. Musanayambe autoclaving, zilowerereni m'madzi a DEPC usiku wonse kuti muchotse RNase. Mukachotsa DEPC tsiku lotsatira, ikani mu bokosi laling'ono la pipette kuti muchepetse kutentha kwa chinyezi. 121 madigiri, 15-20 mphindi. Pofuna kupewa vuto la nthunzi wamadzi, nyuzipepala zimatha kukulunga pabokosi laling'ono, kapena kuziyika mu chofungatira kuti ziume pambuyo potseketsa. Ndi bwino kuti samatenthetsa mwachindunji aliyense m'zigawo, ndipo musagwiritse ntchito nthawi yaitali nsonga pipette kuchotsa RNA.
Ubwino wa sterilization yotentha kwambiri:
Kulowa kwamphamvu kwa kutentha kwa nthunzi; mkulu wotseketsa bwino; nthawi yochepa yolera; palibe mankhwala kapena kuipitsidwa kwa thupi panthawi yotseketsa; zochepa zowongolera zida zotsekereza ndi ntchito yokhazikika; kutsekereza kwa nthunzi kumagwiritsidwa ntchito kusunga madzi ndi mphamvu. Kutentha kwapamwamba kwambiri.
Malangizo a pipette a Yongyue amapangidwa ndi zinthu zachipatala za polypropylene (PP), zomwe zimakumana ndi kalasi ya USP VI, zimakhala bwino kwambiri ndi mankhwala, ndipo zimatha kutsukidwa pa kutentha kwa madigiri 121 ndi kuthamanga kwambiri (mankhwala amtundu wa ma elekitiroti oletsa kulera) .
Nthawi yotumiza: Nov-02-2021