Kodi mumadulansonga ya pipettepamene pipeting glycerol? Ndidachita panthawi ya PhD yanga, koma ndidayenera kuphunzira kuti izi zimawonjezera kusalondola komanso kulondola kwa mapaipi anga. Ndipo kunena zoona ndikamadula nsonga, ndikadathiranso molunjika glycerol kuchokera mubotolo kupita mu chubu. Kotero ine ndinasintha njira yanga kuti ndipititse patsogolo zotsatira za pipetting ndikupeza zotsatira zodalirika komanso zowonjezereka pamene ndikugwira ntchito ndi zakumwa za viscous.
Gulu lamadzimadzi lomwe limafunikira chisamaliro chapadera pamene pipetting ndi zakumwa za viscous. Izi nthawi zambiri zimagwiritsidwa ntchito mu labu, mwina mwanjira yoyera kapena ngati zigawo za buffer. Oimira odziwika bwino a zakumwa zoledzeretsa m'ma laboratories ofufuza ndi glycerol, Triton X-100 ndi Tween® 20. Komanso, ma laboratories omwe amayendetsa bwino zakudya, zodzoladzola, mankhwala ndi zinthu zina zogula amakumana ndi mayankho a viscous tsiku lililonse.
Viscosity imatchulidwa ngati kukhuthala, kapena kukhuthala kwa kinematic. M'nkhaniyi ine amaganizira zamphamvu mamasukidwe akayendedwe a zakumwa chifukwa limafotokoza kayendedwe ka madzi. Mlingo wa mamasukidwe akayendedwe amafotokozedwa mu millipascal pamphindi (mPa * s). M'malo mwake zitsanzo zamadzimadzi zozungulira 200 mPa*s monga 85 % glycerol zitha kusamutsidwabe pogwiritsa ntchito pipette yachikale ya air-cushion. Mukamagwiritsa ntchito njira yapadera, kusinthasintha kwa pipetting, kukhumba kwa thovu la mpweya kapena zotsalira pansonga kumachepetsedwa kwambiri ndipo kumabweretsa zotsatira zolondola kwambiri za pipetting. Komabe, si zabwino zomwe tingachite kuti tipititse patsogolo kutulutsa kwamadzimadzi a viscous (onani mkuyu 1).
Pamene viscosity ikuwonjezeka, zovuta zimawonjezeka. Mayankho apakati a viscous mpaka 1,000 mPa*s ndiovuta kusamutsa pogwiritsa ntchito ma pipette apamwamba kwambiri a air cushion. Chifukwa cha kukangana kwakukulu kwa mkati mwa mamolekyu, zakumwa za viscous zimakhala ndi khalidwe loyenda pang'onopang'ono ndipo pipetting iyenera kuchitika pang'onopang'ono komanso mosamala. Njira yosinthira mapaipi nthawi zambiri siyokwanira kusamutsa madzi molondola ndipo anthu ambiri amayezera zitsanzo zawo. Njirayi imatanthauzanso kutenga kachulukidwe ka madziwo komanso momwe zinthu zilili mu labotale monga chinyezi ndi kutentha kuti muwerengere bwino kuchuluka kwamadzi ofunikira kulemera. Chifukwa chake, zida zina zapaipi, zomwe zimatchedwa zida zosinthira zabwino, zimalimbikitsidwa. Izi zili ndi nsonga yokhala ndi pistoni yophatikizika, ngati syringe. Chifukwa chake, madzi amatha kufunidwa mosavuta ndikuperekedwa pomwe kusamutsa kwamadzimadzi kumaperekedwa. Njira yapadera sikofunikira.
Komabe, zida zabwino zosinthira zimafika malire ndi mayankho owoneka bwino monga uchi wamadzimadzi, zonona pakhungu kapena mafuta ena amakina. Zamadzimadzi zomwe zimafunikira kwambiri izi zimafunikira chida china chapadera chomwe chimagwiritsanso ntchito njira yabwino yosamutsira koma komanso chimakhala ndi kapangidwe koyenera kuthana ndi mayankho owoneka bwino. Chida chapaderachi chikufaniziridwa ndi malangizo omwe alipo kuti apeze malo omwe ndikofunika kuti musinthe kuchoka ku nsonga yachibadwa kupita ku nsonga yapadera ya zothetsera zowoneka bwino. Zinawonetsedwa kuti kulondola kumawonjezeka ndipo mphamvu zomwe zimafunikira pakulakalaka ndi kugawa zimachepetsedwa mukamagwiritsa ntchito nsonga yapadera yamadzimadzi owoneka bwino kwambiri. Kuti mumve zambiri komanso zitsanzo zamadzimadzi, chonde tsitsani Applicaton Note 376 pakuchita bwino pazamadzimadzi zowoneka bwino.
Nthawi yotumiza: Jan-23-2023