Polymerase chain reaction (PCR) ndi njira yomwe imagwiritsidwa ntchito kwambiri ndi ofufuza zamankhwala, asayansi azamalamulo komanso akatswiri azachipatala.
Potchulapo ntchito zake zochepa, zimagwiritsidwa ntchito polemba ma genotyping, kutsatizana, kupanga ma cloning, ndi kusanthula mafotokozedwe a jini.
Komabe, kulemba machubu a PCR ndikovuta chifukwa ndi ang'onoang'ono ndipo ali ndi malo ang'onoang'ono osungiramo zambiri.
Pomwe, mbale za skirted quantitative PCR (qPCR) zitha kulembedwa mbali imodzi yokha.
Mufunika cholimba, chokhazikika PCR chubukuti mugwiritse ntchito mu labotale yanu? Yesetsani kutsata wopanga wotchuka.
Phukusi Lonse
PCR-Tag Trax yomwe ikudikirira patent ndiye njira yaposachedwa kwambiri komanso yabwino kwambiri yolembera machubu apamwamba a PCR, mizere, ndi ma qPCR
Mapangidwe osinthika a tag osamatira amathandizira kuzindikira machubu a PCR apamwamba a 0.2 ml ndi mbale za qPCR zopanda masiketi mumasinthidwe osiyanasiyana.
Phindu lalikulu la PCR-Tag Trax ndikutha kupereka malo okwanira osindikizira kapena, ngati kuli kofunikira, kulemba pamanja.
Pogwiritsa ntchito chosindikizira chosinthira kutentha, ma tag amatha kusindikizidwa ndi manambala osasintha komanso ma barcode a 1D kapena 2D ndipo amatha kupirira kutentha mpaka -196 ° C komanso mpaka +150 ° C.
Izi zimawapangitsa kukhala ogwirizana ndi ma cyclers ambiri a thermo. Ndibwino kuyesa zitsanzo zama tag m'ma cyclers anu a thermo kuti muwonetsetse kuti sizikusokoneza zomwe zimachitika.
Ayenera kukhala ochezeka ndi ma glovu, apereke mawonekedwe a mbalame mwachangu pazomwe zalembedwa pama tag pomwe ma thermo cyclers atsegulidwa.
Machubu a PCR amatha kukhala amitundu yosiyanasiyana kapena amitundu yambiri kuti alembe mosavuta.
Ma tag opanda zomatira amathanso kugwiritsidwa ntchito ngati chothandizira machubu anu, kupangitsa kuti zikhale zosavuta kuyika ma reagents a pipette mkati mwawo ndikuzisunga mufiriji kapena mufiriji mutayankha.
Machubu a PCR, 0.2mL
Machubu a PCR pawokha amatha kulembedwa pamalo awiri osiyana: machubu ndi kapu yake.
Kuti mulembe mitundu yosavuta, zilembo zam'mbali zamachubu ang'onoang'ono a PCR zimapezeka mumitundu ingapo pa makina osindikizira a laser ndi thermal-transfer.
Zambiri zitha kusindikizidwa pa zilembo za machubu a PCR kuposa momwe zingalembedwe pamanja, ndipo ma barcode atha kugwiritsidwa ntchito kupititsa patsogolo kufufuza.
Zolembazo ndi zotetezeka ndipo zimatha kusungidwa mufiriji labu kwa nthawi yayitali.
Zolemba za madontho ozungulira ndiye chisankho chabwino kwambiri cholembera nsonga za machubu a PCR.
Zolemba za madontho, kumbali ina, zimakhala ndi malo ochepa pa chubu kuti asindikize kapena kulemba zambiri. Chifukwa chake kuwapangitsa kukhala amodzi mwamachubu ochepera a PCR omwe amalemba machubu.
Ngati muyenera kugwiritsa ntchito madontho a machubu a PCR ndipo mukhala mukulemba ambiri, pikaTAGTM.
pikaTAGTM ndi chipangizo chogwiritsira ntchito chomwe chimatenga madontho madontho mwachindunji kuchokera pamzere wawo ndikumangirira pamwamba pa machubu.
Ili ndi mawonekedwe a ergonomic ngati cholembera chomwe chimapangitsa kulemba madontho mwachangu komanso kosavuta, kuchotsa ntchito yowononga nthawi yosankha zilembo zing'onozing'ono komanso kupewa kuvulala kwapang'onopang'ono komwe kumadza chifukwa cholemba machubu.
Zovala za PCR Tubes
Zingwe za PCR nthawi zambiri zimagwiritsidwa ntchito m'ma lab omwe amapanga njira zambiri za PCR ndi qPCR.
Kulemba zilembo izi kumakhala kovuta kwambiri kuposa kulemba machubu amodzi chifukwa chubu chilichonse chimalumikizidwa ndi china, motero kumachepetsa malo ozindikiritsa omwe adaletsedwa kale.
Mwamwayi, mizere 8 ya machubu imagwirizana ndi chubu chilichonse, zomwe zimapangitsa PCR kukhala ndi kamphepo.
Mizere iyi yopangidwa ndi GA yapadziko lonse lapansi, imakhala ndi ma perforations pakati pa cholembera chilichonse chomwe chili mu mpukutuwo, kukulolani kuti musindikize zolemba zambiri monga pali machubu.
Ikani mzere wonse wa chizindikiro pafupi ndi mbali ya chubu, sungani malemba onse nthawi imodzi, ndiyeno muthyole zowonongeka kuti zilembozo zikhale zolimba pambali.
Pakutentha kwa -80°C mpaka +100°C, malembo osindikizika otenthetserawa ndi otetezeka kuti agwiritsidwe ntchito m'ma cyclers a thermo ndipo akhoza kusungidwa bwino mufiriji wa mu labotale.
Njira Yachikhalidwe
Kulemba pamanja ndiyo njira yodziwika kwambiri yozindikirira machubu a PCR, ngakhale kuti sikoyenera chifukwa kulemba movomerezeka pamachubu a PCR sikutheka.
Kulemba pamanja kumachotsanso ma serialization ndi ma barcode, zomwe zimapangitsa kuti zikhale zovuta kutsatira zitsanzo zanu.
Ngati kulemba ndi njira yokhayo pa labu yanu, zolembera zabwino za cryo ndizoyenera kuyikapo ndalama chifukwa zimakulolani kulemba momveka bwino momwe mungathere popanda kuzimiririka kapena kusawoneka bwino.
Lumikizanani nafe machubu apamwamba kwambiri a PCR
Timapanga ndi kupanga apamwambaMachubu a PCRkuti zigwiritsidwe ntchito mu genotyping, sequencing, cloning, ndi kusanthula majini m'ma laboratories osiyanasiyana azachipatala ndi malo ofufuza.
Kuti mudziwe bwino ndi machubu a PCR, chitanikufikira kwa ife chifukwa cha chinthu chabwino komanso chogwira ntchito.
Nthawi yotumiza: Oct-30-2021