Makina okhudzana ndi polymemet (PCR) ndi njira yomwe imagwiritsidwa ntchito kwambiri ndi ofufuza zasayansi, wasayansi komanso akatswiri azachipatala.
Kuwerenga ndalama zina mwa njira zake, imagwiritsidwa ntchito ngati genotyping, zotupa, kulowerera, ndi kusanthula kwamitundu.
Komabe, kulembera machubu a PCR ndikovuta chifukwa ndi zazing'ono ndipo kumakhala ndi malo ochepa posungira zambiri.
Pomwe, kudumphadumpha pcr (qpcr) kungolembedwa mbali imodzi
Kodi mukufuna kukhala olimba, okhazikika PCR chubuZogwiritsidwa ntchito mu labotale yanu? Yesetsani kuyang'anira wopanga yemwe anali wodziwika.
Phukusi lonse
PCR-Tag Trax ndi njira yaposachedwa kwambiri komanso yabwino kwambiri yolembera machubu apamwamba a PCR, mabala, ndi qpcr plats
Makina osamatira a zotsatsa amathandizira kuzindikira 0,2 ml kukwera machubu a PCR ndi mabatani osakhazikika a QPCR pakusintha kosiyanasiyana.
Ubwino waukulu wa pcr-tag trax ndi kuthekera kwake kupereka malo okwanira kusindikiza kapena, ngati kuli kotheka, zolemba pamanja.
Pogwiritsa ntchito chosindikizira cha matenthedwe, ma tag amatha kusindikizidwa ndi kuchulukana kogwirizana ndi 1d kapena 2d ma barcode ndipo amatha kupirira kutentha ngati -10% ngati + 150 ° C.
Izi zimapangitsa kuti azigwirizana ndi ma arro ozungulira. Ndi lingaliro labwino kuyesa zitsanzo za ma tag omwe ali nawo ozungulira a thermo cyclers kuti mutsimikizire kuti sasokoneza zomwe zingachitike.
Ayenera kukhala ochezeka
Mabatani a PCR amatha kubwera m'malo osiyanasiyana kapena mtundu wa mitundu yambiri yolemba mtundu wosavuta.
Tags zomata zomatira zitha kugwiritsidwanso ntchito ngati chithandizo cha machubu anu, ndikupangitsa kuti zikhale zosavuta kwa ma pipette amakonzanso mufiriji kapena freezer pambuyo pochita izi
Mabatani a PCR, 0.2ML
Mabatani a PCR payekha amatha kulembedwa pamitundu iwiri: machubu ndi chipewa chake.
Mwa kusala kwabwino kwapadera, zolembera zam'mbali za machubu aang'ono a PCR zikupezeka mu mitundu ingapo ya asitikali onse a laser ndi osindikiza.
Zambiri zitha kusindikizidwa pa zilembo za PCR Chubu kuposa dzanja, ndipo ma barcode angagwiritsidwe ntchito kusintha kusokonekera.
Zolemba ndi zotetezeka ndipo zimatha kusungidwa mu lab freezers kwa nthawi yayitali.
Zolemba zozungulira za DOT ndiye chisankho chabwino kwambiri cholemba PCR chubu Tsamba.
Mapepala a DOT, kumbali inayo, amakhala ndi malire ochepa pa chubu kuti asindikize kapena kulemba zambiri. Chifukwa chake apangeni amodzi mwa mababu okwanira a PCR polemba zosankha.
Ngati muyenera kugwiritsa ntchito zikwama za DCR ndikulemba ambiri aiwo, pikatagtm.
Pikatagtm ndi chipangizo chofunsira chomwe chimatenga zilembo za dot mwachindunji ndikuwaumba pamwamba pa machubu.
Imadzitamandira mawonekedwe owoneka ngati a ergonon omwe amapanga dontho lachangu komanso losavuta, kuchotsa ntchito yowononga nthawi yopanga zigawo zazing'ono zomwe zimayambitsa matenda obwera chifukwa cha chubu.
Mabatani a pcr
Masamba a PCR nthawi zambiri amagwiritsidwa ntchito mu labs yomwe imapha pcr ndi qpcr.
Kulemba mavuko kumakhala kovuta kwambiri kuposa kulembera machubu amodzi chifukwa chubu chilichonse chimalumikizidwa ndi lotsatira, popewa kuzindikiritsa malo omwe achepetsedwa.
Mwamwayi, 8-pindable mbiri imagwirizana ndi chubu lililonse, ndikupanga zikwangwani za PCR yolemba kamphepo.
Zolemba izi zopangidwa ndi gal International, kukhala ndi zojambula pakati pa zilembo zilizonse pamtengo, ndikulolani kusindikiza ngati zilembo zambiri monga mabatani ambiri.
Ikani mzere wonse wa zilembo pafupi ndi chubu cha chubu, gwiritsitsani zilembo zonse nthawi yomweyo, kenako ndikuphwanya zojambulazo kuti zilembedwe zikuluzikulu.
Pa kutentha kwa-30 ° C kwa + 100 ° C, kutsatsa kwa zojambulazo kumakhala kotetezeka kugwiritsa ntchito ma armo cyclers ndipo atha kusungidwa bwino m'madzi a labotale.
Njira yachikhalidwe
Zolemba pamanja ndi njira yofala kwambiri yodziwitsa machubu a PCR, ngakhale siyikhala yabwino chifukwa polemba mogwirizana ndi machubu a PCR ndizosatheka.
Zolemba pamanja zimachotsanso kusumirira ndi mabizinesi, kupangitsa kuti zikhale zovuta kuzifufuza zitsanzo zanu.
Ngati zolembedwa pamanja ndiye chisankho chokhacho cha labu yanu, zolembera zabwino kwambiri zamiyala ndizoyenera kuyikapo kanthu chifukwa zimakupatsani mwayi kuti mulembe molakwika momwe mungathere popanda kuzimiririka kapena kuwonongeka.
Lumikizanani nafe kwa machubu apamwamba kwambiri a PCR
Timapanga ndikupanga zabwino kwambiriMachubu a PCRKuti mugwiritse ntchito mu genotyping, kupindika, kulonga, ndi kusanthula kwa majini mu labotale yamankhwala ndi Sufufute.
Pazochitika zabwino kwambiri ndi machubu a PCR, chitanikufikira kwa ife kwa chinthu chabwino komanso ntchito.
Post Nthawi: Oct-30-2021