CROVINILSamagwiritsidwa ntchito posungira ma chrisgegenic a convinege mizere ndi zina zakuthambo, m'matanda amadzazidwa ndi nayitrogeni yamadzi.

Pali magawo angapo omwe amakhudzidwa ndi kukonza bwino maselo mu nayitrogeni. Pomwe mfundo yoyamba ndi yowuma pang'onopang'ono, njira yeniyeni yogwiritsidwa ntchito imatengera mtundu wa khungu ndi crroprotector omwe amagwiritsidwa ntchito. Pali malingaliro angapo achitetezo komanso machitidwe abwino kuti muganizire mukamasunga maselo pamitedwe yotsika kwambiri.

Izi zikuwonetsa kuti mupereke chithunzithunzi cha momwe ma cryvoals amasungidwa mumadzi a nayitrogeni.

Ma cryvials ndi chiyani?

CROVINALS ndi zazing'ono, zojambulidwa bwino zopangika zitsanzo zamadzimadzi nthawi yochepa kwambiri. Amawonetsetsa kuti maselo amasungidwa mu chroprotectic osalumikizana mwachindunji ndi nayitrogeni wamadzimadzi, kuchepetsa chiopsezo cha ma cell a cellular pomwe akupindula ndi zozizira kwambiri za nayitrogeni wamadzi.

Mbale nthawi zambiri zimapezeka mu mavoliyumu osiyanasiyana ndi mapangidwe - amatha kukhala opanda thupi kapena kunja kwa boloni lathyathyathya kapena lozungulira. Mitundu yosabala komanso yosabala imapezekanso.

Omwe amagwiritsa ntchitoCyrvaalskusunga maselo mu nitrogen yamadzi

Mitundu yambiri ya NHS ndi malo osungirako zachinsinsi, komanso mabungwe ofufuzira magazi, khungu la epithelial ndi stem cell cell celevial ​​gwiritsani ntchito cripre olema.

Maselo omwe amasungidwa mwanjira imeneyi amaphatikizapo maselo a B ndi T, Choti Maselo a THIBUPORTC, MacOpenitor, machesi, atheloma, maselo a myeloma ndi maselo ang'onoang'ono.

Mwachidule momwe mungasungire cravoals mu nitrogen yamadzi

Crowpo Persection ndi njira yomwe imasunga maselo ndi zina zowonjezera powazizira kuti azitentha kwambiri. Maselo amatha kusungidwa m'madzi a nayitrogeni kwa zaka popanda kutayika kwa maselo. Ichi ndi chithunzi cha njira zomwe zimagwiritsidwa ntchito.

Kukonzekera kwa cell

Njira yeniyeni yokonzekera zitsanzo zimasiyana malinga ndi mtundu wa khungu, koma zambiri, maselo amatengedwa ndipo centrifull kuti apange pellet wolemera. Pellet iyi imasinthidwa mu supernatant yosakanizidwa ndi crrisprrotecticject kapena sing'anga.

Criprerecrecy sing'anga

Sing'anga iyi imagwiritsidwa ntchito kuti isungidwe maselo osungira mu malo otsika-kutentha kwambiri omwe aperekedwa poletsa mapangidwe a intra ndi makhiristo akunja. Udindo wawo ndikupereka malo otetezeka, oteteza ma cell ndi minyewa mu nthawi yozizira, yosungirako, ndikung'amba njira.

Sing'anga monga plasma yatsopano (FFP), mayankho a plasmalyte;

Samp-StudPed Pellet imakondwera mu polyproplene cravovials mongaSuzhou Ace Biomedal Company Crowgenic Yosungira.

Ndikofunika kuti musamadzichepetse za chrovavaals popeza izi zikuwonjezera chiopsezo cha zomwe zilipo komanso zomwe zatulutsidwa (1).

Kuongoleredwa kwaulere

Mwambiri, kuchepa kwaulere kumagwiritsidwa ntchito pochita bwino maselo.

Pambuyo pa zitsanzo zokhala ndi ma crygenic mbale, zimayikidwa pa ayezi wonyowa kapena mufiriji 4 ℃ ℃ firiji ndipo njira yozizira imayambika mkati mwa mphindi 5. Monga kalozera wamba, maselo amakhazikika pamlingo wa -1 mpaka -3 pamphindi (2). Izi zimatheka pogwiritsa ntchito coorm yolumikizira kapena poika mbale m'bokosi lotayika lomwe limayikidwa mu -70 ° C mpaka -90 ° C mpaka -90 ° C mpaka -90 ° C mpaka -90

Sinthani ku nayitrogeni yamadzi

Mbale zophukira zophukira zimasamutsidwa ku tank ya nitrogen nthawi zonse nthawi zonse zimapereka kutentha kwa -135 ℃ kumasungidwa.

Matenthedwe otsika kwambiri amatha kupezeka mwa kumizidwa mu madzi kapena mpweya wa nayitrogeni.

Gawo lamadzi kapena nthunzi?

Kusungidwa mu Gawo la Nitrogen kumadziwika kuti kutentha kuzizira kosasinthika, koma nthawi zambiri sikulimbikitsidwa pazifukwa zotsatirazi:

• Kufunika kwa mavoliyumu akuluakulu (kuya) kwa nayitrogeni yamadzi omwe ndi ngozi yomwe ingachitike. Kuwotcha kapena kubisa chifukwa cha izi ndi chiopsezo chenicheni.
• Milandu yolembedwa yoipitsidwa ndi matenda opatsirana monga a asrpilgillus, hep b ndi viral idafalikira kudzera pa nayitrogeni ya nitrogen (2,3)
• Kuthekera kwa madzi nayitrogeni kuti atuluke mu mbale mumimba. Akachotsedwa kusungira kutentha, nayitrogeni msanga. Zotsatira zake, vial itha kusweka ikachotsedwa pamtunda wa nayitrogeni madzi, ndikupanga zoopsa kuchokera ku zinyalala zonse zouluka ndi kuwonekera kwa zomwe zili (1, 4).

Pazifukwa izi, kutentha kwa kutentha kochepa kwambiri kumakhala kofala kwambiri m'mphepete mwa nayitrogeni. Pamene zitsanzo ziyenera kusungidwa mu gawo la madzi, ma cry cnseble tubing ayenera kugwiritsidwa ntchito.

Chotsika ndi mtunda wa mpweya ndikuti kutentha kutentha kumatha kuchitika chifukwa chosinthasintha pakati -135 ℃ ndi -190 ℃. Izi zimafunikira kuwunikira mosamala komanso mwakhama zamadzimadzi ndi mitundu yosiyanasiyana (5).

Opanga ambiri amalimbikitsa kuti ma cryvovials ali oyenera kusungidwa mpaka -135 ℃ kapena kuti agwiritsidwe ntchito m'gulu la nthunzi kokha.

Kuchepetsa maselo anu a Crypropreser

Njira yoyeserayo ndi yovuta kwa chikhalidwe chonyansa, ndipo magwiridwe antchito abwino ndi luso limafunikira kuti amvetsetse bwino kwambiri, kuchira, komanso magwiridwe antchito a maselo. Ma protocol enieni amadalira mitundu ya khungu. Komabe, kuchepa kofulumira kumaonedwa kuti:

• Chepetsani zomwe zimachitika pakubwezeretsa ma cell
• Thandizani kuchepetsa nthawi yowonekera kwa zosungunulira zomwe zilipo mu media
• Chepetsani kuwonongeka kulikonse ndi ma ayezi recerystalistion

Malo osambira madzi, malo osambira madzi, kapena zida zapadera nthawi zambiri amagwiritsidwa ntchito ku Thaw zitsanzo.

Nthawi zambiri mzere wa maselo umodzi umatha nthawi ya mphindi 1-2, ndikusenda pang'ono musamba 37 ℃ madzi pang'ono pokhapokha mutangotsukidwa mu vial asanatsukidwe.

Kwa maselo ena monga mazira am'madzi, pang'onopang'ono kutentha ndikofunikira kuti apulumuke.

Maselo tsopano ali okonzekera chikhalidwe cha maselo, kudzipatula kwa maselo a haematopopoietic.

Ndichinthu chabwinobwino kuti mutenge maulendo ang'onoang'ono a zitsanzo zowoneka bwino zomwe zimagwiritsidwa ntchito popanga maselo kuti mudziwe zozunzira maselo am'manja. Mutha kuyesa zotsatira za njira zolerera ndi mawonekedwe a cell ndikudziwa kusintha kwa maselo.

Machitidwe abwino osungira marpovalials

Kukula kwa zitsanzo zoyendetsedwa ndi zitsanzo zosungidwa mu CROVVials zimatengera zinthu zambiri mu protocol kuphatikiza kusunga koyenera komanso kusunga mbiri yabwino.

• Gawani maselo pakati pa malo osungirako- Ngati ma voliyumu amalola, kudutsa maselo pakati pa mitsuko ndikuwasunga m'malo osiyanasiyana kuti muchepetse kuwonongeka kwa zikondwerero chifukwa cha zolephera za zida.
• Pewani kuipitsidwa- Sankhani osasunthika osasunthika kapena radilave musanayambe kugwiritsa ntchito
• Gwiritsani ntchito Mbale Zoyenera Zithunzi Zanu- Mbale zimabwera m'mawu osiyanasiyana pakati pa 1 ndi 5mls. Pewani zochulukirapo zochulukirapo kuti muchepetse chiopsezo cha kusweka.
• Sankhani Mbale Zapakatikati- Mbale zopsinjika mkati mwa mkati zimatsimikiziridwa ndi mayunivesite ena otetezeka - amathanso kuletsa kuipitsidwa mukamadzazidwa kapena ikasungidwa mu nayitrogeni yamadzi.
• Pewani kutaya- Gwiritsani ntchito zisindikizo za BI-zopangidwa ndi zingwe zomwe zimapangidwa mu screw-cap kapena ma o-mphete kuti mupewe kuwuma ndi kuipitsidwa.
• Gwiritsani ntchito ma barcode a 2d ndi zilembo za zilembo- Kuonetsetsa kuti nditakhala ndi vuto, Mbale zomwe zili ndi madera akuluakulu olemba zimapangitsa kuti aliyense akhale wokwanira. 2D Barcodes amatha kuthandiza poyang'anira ndi mbiri yolemba mbiri. Ziphuphu zokongoletsera za utoto ndizothandiza pakudziwika bwino.
• Kukonza kokwanira- Kuonetsetsa maselo osatayika, zombo zosungidwa ziyenera kuwunika kutentha ndi kuchuluka kwa nayitrogeni. Ma alarm ayenera kukhala oyenera kuchenjeza ogwiritsa ntchito zolakwika.

Kusamala

Nitrogen yamadzimadzi yakhala yofala mu kafukufuku wamakono koma zimanyamula chiopsezo chovulala kwambiri ngati ntchito molakwika.

Zida zoteteza payekha (PPE) ziyenera kuvalidwa kuti muchepetse chiopsezo cha chiopsezo, kuwotcha ndi zochitika zina zovuta mukamagwira nitrogen yamadzi. Vala

• Magolovesi a crorgenic
• Chovala cha labotale
• Chikopa cholimbana ndi nkhope yonse zomwe zimaphimba khosi
• Nsapato zotsekedwa
• Splarsproof pulasitiki

Maffere a nitrogen a nitrogen ayenera kuyikidwa m'malo opumira bwino kuti muchepetse chiopsezo cha End Expriation - nayitrogeni amachotsa mpweya wabwino. Malo ogulitsira ambiri ayenera kukhala ndi ma alasgen ocheperako.

Kugwira ntchito awiriawiri mukamagwira nitrogen madzi nitrogen ndi abwino ndipo kugwiritsa ntchito kwake kunja kwa maola wamba kuyenera kuletsedwa.

CROVVICUCS kuti muthandizire ntchito yanu

Kampani ya Suzhou Ace Biomedical Company imapereka zinthu zina zosankhidwa zomwe zimakwaniritsa zosowa zanu zamitundu yosiyanasiyana. Malangizowo amaphatikizanso mabatani osiyanasiyana ngati miyeso yosiyanasiyana.

Msinkhuwu wathu ndi:

• Labu scress cap 0.5ml 1.5ml 2.0ml crykovial crogenic Mbale zokhala ndi ma cryt

  ● 0.5ml, 1.5ml, 2.0ml, wokhala ndi siketi kapena wopanda siketi
  ● Mawonekedwe oyenera kapena odziyimira kapena osabala kapena osabala omwe alipo
  ● Desitsani kapu machubu opangidwa ndi kalasi yachipatala polypropylene
  ● Mbale za PP Crowwobe zitha kukhala zoundana mobwerezabwereza
  ● Kapangidwe kakang'ono kwakunja kumatha kuchepetsa zovuta zoipitsa panthawi yachitsanzo.
  ● Chongani Cap Chrogenic Machubu a chilengedwe chonse kuti agwiritse ntchito
  ● Machubu amakwanira zowola kwambiri
  ● Christogenic chubu o-mphete yoyenera 1-inche ndi 2-inchi, 4ill, 81weewe, 96wewewer ndi 100 freezer
  ● Autoclalated mpaka 121 ° C ndi ozizira ku -86 ° C

  Gawo ayi	Malaya	Phokoso	ChipewaMtundu	PCS /Thumba	Matumba / mlandu
  Act055-bl-n	PP	0.5ml	Wakuda, wachikasu, wabuluu, wofiira, wofiirira, woyera	500	10
  Act15-bl-n	PP	1.5ml	Wakuda, wachikasu, wabuluu, wofiira, wofiirira, woyera	500	10
  Act15-blo-nw	PP	1.5ml	Wakuda, wachikasu, wabuluu, wofiira, wofiirira, woyera	500	10
  Act20-bl-n	PP	2.0ml	Wakuda, wachikasu, wabuluu, wofiira, wofiirira, woyera	500	10