Zomwe zimagwiritsidwa ntchito ndi reagent ndi chimodzi mwazinthu zomwe zimagwiritsidwa ntchito kwambiri m'makoleji ndi ma labotale, komanso ndi zinthu zofunika kwambiri kwa oyesera. Komabe, kaya zogula za reagent zimagulidwa, kugulidwa kapena kugwiritsidwa ntchito, padzakhala zovuta zingapo pamaso pa oyang'anira ndi ogwiritsa ntchito zinthu zomwe zimagwiritsidwa ntchito. Mavuto enieniwo ndi ati? Ndiroleni ndikufotokozereni mwachidule.
Kugula ma reagents ndi zogwiritsidwa ntchito, chifukwa cha chidziwitso chawo cha asymmetry, kuphatikiza kuti wopereka ma reagents ndi zogwiritsidwa ntchito adalemba ganyu ogulitsa kuti agulitse, mitengo imakwezedwa pambuyo wosanjikiza ndi wosanjikiza wa kukwera kwamitengo. Zotsatira zake, mtengo wogula reagent womwewo m'ma laboratories awiri pafupi ndi yunivesite / labotale yomweyi pamtunda womwewo ndi wosiyana kwambiri. Kuphatikiza apo, akatswiri ambiri asayansi ofufuza / oyesa sanathe kuzindikira ziyeneretso za woperekayo, zomwe zidapangitsa kuti alandire [katundu wabodza” ndi [kutuluka kunja kofanana”. Pamapeto pake, adagwira ntchito molimbika kwa zaka zoposa theka la zoyeserera, koma zotsatira za zoyesererazo zidatha chifukwa adagula ma reagents abodza. zosavomerezeka. Kunyenga kwa zinthu zogwiritsira ntchito reagent kumakhudza kwambiri kafukufuku wa sayansi ndi zotsatira za mayesero, ndipo si zachilendo kuti ochita kafukufuku awononge nthawi yambiri, ndalama, ndi mphamvu pa kafukufuku yemwe sali wothandiza. Njira zina zabodza ndizobisika kwambiri. Zida za ELISA izi zigwiritsanso ntchito zolozera zina kuti zitsanzire zida zina zolozera. Koma poyerekeza ndi njira yapitayi ya VEGF, yomwe ili phukusi lazinthu, "wanzeru" ndikuti zizindikiro zomwe zimagwiritsidwa ntchito m'malo zimakhala zosiyana kwambiri komanso zobisika, zomwe zimakhala zovuta kuzipewa.
Ndiye ndingapeze bwanji ma reagents enieni ndi zogwiritsidwa ntchito kuti ndipewe kupusitsidwa? Nazi njira zingapo:
1. Pezani zogulitsira zoyenera ndi ogulitsa reagent
Mukamagula zinthu zogwiritsira ntchito reagent, muyenera kupewa kugula zinthu zabodza za reagent kuchokera kugwero. Chifukwa chake, momwe mungasankhire wopereka woyenera wa ma reagents ndi zogwiritsidwa ntchito ndizofunikira kwambiri. Kusankhidwa kwa ogulitsa kungakhazikitsidwe pa mfundo ziwiri: 1 ndikusankha mitundu iwiri yayikulu ndi ogulitsa omwe ali ndi mbiri yabwino; 2 kuti akhazikitse njira yabwino yoyendetsera zinthu. Khazikitsani miyezo yowunika kwa ogulitsa ndi ogulitsa, kulembetsa mtundu wamtundu uliwonse wa reagent ndi zogwiritsidwa ntchito, ndikukhala ndi njira zolangira zophwanya, monga kuwaletsa kutenga nawo gawo pakuyitanitsa ndi kupereka panthawi yopereka. Kusankha ogulitsa oposa awiri kungagwiritsidwe ntchito kufananitsa ubwino ndi mtengo wa magulu awiriwa, kuti apereke zosankha zabwino komanso zowonjezereka kwa ogwira nawo ntchito m'mayunivesite / ma lab.
2. Phunzirani luso losavuta lozindikiritsa
Pali njira zambiri zozindikiritsira ma reagents ndi zogwiritsidwa ntchito. Zotsatirazi ndi mndandanda wachidule wa ziwiri:
1. Yang'anani pa paketi
Tikapeza ma reagents ndi zogwiritsidwa ntchito, choyamba tiyenera kutsimikizira kuti chisindikizocho sichinang'ambika kapena palibe njira zina zoyendayenda. Mukawona ngati pali zisindikizo zilizonse zosunthidwa, samalani ngati mizere yachisindikizo ndi zithunzi zikugwirizana bwino. Ngati mizere yamapangidwe ndi zithunzi sizikufanana, kulongedzako sikunachitike.
2. Yang'anani pa chizindikiro cha kusintha kwa mtundu/chokutira chotsutsana ndi kuba
Njira yodziwika bwino yodziwira zinthu zomwe zimagwiritsidwa ntchito ndi reagent ndikusintha mawonekedwe owonera, ndipo mutha kuwona kuti cholembera chotsutsana ndi chinyengo chosintha mtundu chikuwoneka mumitundu iwiri yotsatirayi. Choyamba, chotsani "chophimba chotsutsana ndi chinyengo" pa phukusi kuti mutenge kachidindo kotsutsa, ndikulowetsani ku tsamba lovomerezeka kuti muwone.
Nthawi yotumiza: Aug-09-2022