MMENE MUNGAYELEKE KUYANG'ANIRA 96 DEEP WELL PLATE

Ndi maola angati pa sabata mumataya mbale zakuya?

Kulimbana ndi zenizeni. Ziribe kanthu kuchuluka kwa mapaipi kapena mbale zomwe mwanyamula mu kafukufuku kapena ntchito yanu, malingaliro anu atha kuyamba kusewera pa inu pankhani yokweza mbale yowopsa ya 96.

Ndizosavuta kuwonjezera ma voliyumu pachitsime cholakwika kapena mzere wolakwika. Ndizosavuta kuwirikiza mwangozi mbale yakuya yomweyi.

Kapena mumayika zitsanzo zonse zolakwika m'zitsime zingapo, zomwe zimakuwonongerani maola ambiri ogwirira ntchito.

Kapena, mwina munachita zonse bwino, koma mumayamba kudziganizira nokha. Kuyambanso.

Nthawi yanu ndiyofunika kwambiri. Ma reagents anu ndi amtengo wapatali kwambiri. Ndipo, chofunika kwambiri, deta yanu ndi yamtengo wapatali kwambiri.

Sitiyenera kukuuzani kuti izi ndizowononga nthawi, pamene nthawi zambiri mumayenera kupanga ma reagents ndikusakaniza. Komanso, sizimamva bwino kwambiri pamlingo wodalirika.

Nawa maupangiri ndi zidule zabwino kuchokera kwa ena omwe mungayambe kuwaphatikiza muzochita zanu za labu.

Kodi mbale ya 96 yakuya ndi chiyani?

Chokhazikika chomwe nthawi zambiri chimanyalanyazidwa m'ma lab ndi malo opangira kafukufuku kulikonse, mbale zakuya zakuya zomwe zimasungidwa kwakanthawi kochepa komanso kwanthawi yayitali, kukonzekera, ndi kusakaniza. Zitha kukhala ndi chitsime chozungulira kapena chozungulira pansi.

Kugwiritsiridwa ntchito kwawo kumasiyana, koma nthawi zambiri kumagwiritsidwa ntchito pa sayansi ya moyo ndikugwiritsa ntchito kafukufuku, kuphatikizapo:

• Ntchito ya chikhalidwe cha maselo a minofu ndi kusanthula maselo
• Ma enzyme
• Maphunziro a proteinomics
• Malo osungiramo reagent
• Zosungirako zotetezedwa (kuphatikiza zosungirako za cryogenic)

Malangizo apamwamba & zidule zogonjetsera zolakwika 96 za mbale zakuya

Talemba mndandanda wamakina apamwamba ndi njira zochokera kwa anzanu:

1. Yang'anirani malingaliro anu ndikukhala olunjika:Mofanana ndi chilichonse m'moyo, zolakwa zimachitika mukamatopa, kupsinjika, kapena kusokonezedwa (... kapena zonse pamwambapa). Lekani kudandaula kuti muthamangitse ntchito yanu mwachangu. Pepani, ndipo ganizirani mozama za sitepe iliyonse. Ndipo khalani maso. Kulankhula ndi kugwira ntchito kumapangitsa kuti ntchito zina zizipita mwachangu, koma osati ndi ntchitoyi. Ofufuza ena amapachika chizindikiro cha "No talking" alembe pamene ali pakati pa ntchitoyi. Nyimbo zopumula (makamaka zida) zimalimbikitsidwa ngati mukufuna phokoso lakumbuyo mukamagwira ntchito!
2. Gwirizanitsani maupangiri anu a pipette ndi zitsime zofananira:Bokosi latsopano la pipette ndilobwino kwambiri kwa mbale zakuya. Fananizani chitsime ndi bokosi pamene mukupita. Khalani ndi bokosi losunga zobwezeretsera pa standby ngati mwatha, kuti musasokoneze dongosolo lanu ngati mukufuna zambiri. Gwiritsani ntchito malangizo a pipette kuti muwerenge bwino.
3. Lembani:Pangani pepala la Excel la master mix, ndi mamapu 96 akuya a chitsime. Chitsime chilichonse chili ndi dzina la zoyambira ndi zitsanzo. Khazikitsani zosakaniza zanu zonse m'njira yomveka bwino, ndi nambala yamtundu pa seti iliyonse yoyambira (ngati mukugwiritsa ntchito yopitilira imodzi). Bweretsani pepala ili mu labu, ndipo yang'anani chizindikiro papepala pamene mukupita. Mutha kulembanso kuchuluka kwa reagent pa positi ndikuyisunga pafupi ndi inu ngati kiyi yanu yachitsanzo pamene mukukweza. Sankhani dongosolo loti muwagwiritse ntchito (monga motsatira zilembo kapena manambala, kutengera momwe amalembedwera) osasokera pakompyuta yanu. Popanga kusakaniza, ikani zonse mu rack yanu, kenako ndikusunthira ku ngodya yakutali mukamaliza.
4. Tape ndi bwenzi lanu lapamtima:Chotsani mbale yonse, pambali pa malo omwe mukutsitsa. Gwirani ntchito pa mbale motere, ndikusuntha tepi nthawi iliyonse pamene gawo latha. Mutha kulemba tepi yanu (monga A – H, 1 – 12) kuti ikuthandizeni kukhalabe panjira.
   Mwachitsanzo, pamene mukukweza gene A mastermix mu ndime 1 ndi 2 ya mbale yanu yakuya, tengani tepiyo ndikuphimba pang'onopang'ono ndime 3 ndi 4. Mukhozanso kuchita ndime imodziyi nthawi imodzi, kuti mukhale okonzeka. Zimathandizira kukhalabe wolunjika pazitsime zapakati zolimba. Ingokumbukirani kuyika mbaleyo pansi pang'onopang'ono pochotsa tepi yanu, kuti musawope.
5. Khalani nacho:Ngati muwona kuti makina anu sakugwira ntchito, musasinthe pakati. Sinthani musanayambe kapena mutatha, koma osadutsa pakati (zimabweretsa chisokonezo chochuluka!).
6. Yesani:Khalani ogwirizana ndi njira yomwe mwasankha. Kuchita izi kuti mukumbukire minofu kumatenga nthawi, koma pakapita nthawi muyenera kuyamba kuona kusintha kwakukulu pa ntchito yanu (komanso kukhumudwa kwambiri kuntchito kwanu!)

Sankhani zida zoyenera:

Kuchokera kuzinthu mpaka kumtundu, zitsime zozungulira kapena pansi, pali zosankha zingapo poyitanitsa mbale yakuya ya 96.

Zolinga zina ndi izi:

• Zida: Mukugwiritsa ntchito zitsanzo ziti? Kodi chitsime chanu chakuya chiyenera kukutidwa ndi silika kapena chitsulo?
• Kukula: Ndi voliyumu yochuluka bwanji yomwe ikufunika kuti ikwane mu mbale yanu yakuya ya 96 PCR?
• Kutentha: Ndi kutentha kotani komwe zitsime zanu zakuya zimafunikira kupirira?
• Ndi mphamvu ziti za centrifugation zomwe mbale yanu yakuya 96 ingapirire?

Izi ndi zomwe asayansi ambiri amagwiritsa ntchito pazinthu zambiri:

Mbale 96 wa Deep Well awa

Momwe mbale zakuya zimathandizira ma lab ndi oyang'anira ma labu:

• Annjira yosavutakusonkhanitsa ndi kukonza zitsanzo (popeza zinthu zomwe zikuchitika mu labu yanu sizikusowa tsiku lililonse)
• Bweretsaninso labspace yamtengo wapatali, yokhala ndi zosungika zolimba zomwe zimawapangitsa kukhala osavuta kusunga kuposa kale
• Pewani kutaya ndikusakaniza bwinoza zitsanzo zanu zazing'ono zamadzimadzi
• Mapangidwe kutiamachepetsa kusunga makoma, kotero mumawononga zochepa za chitsanzo chanu
• Lipirani33% zochepakuposa momwe mungapangire ma brand ena otsogola

Zina mwazo ndi:

• Pansi yozungulira
• Itha kuzizira kapena firiji (mpaka -80 C)
• Kukhazikika - sangayankhe ndi zosungunulira mu mbale
• Musaphatikizepo zitsulo zolemera kuti muwongolere bwino
• Amapangidwa molingana ndi kukula kwapadziko lonse lapansi (SBS), kuwapangitsa kukhala oyenera malo ogwirira ntchito okha
• Lolani kuti chitsanzo chanu chisasungidwe pang'ono pamakoma

Kusankha mbale yoyenera kungakuthandizeni kupewa:

• Zosowa za data
• Kubwereza kwachitsanzo
• Kuyenda pang'onopang'ono
• Masiku omalizira a polojekiti anaphonya

Wodala pofufuza

96 mbale zakuya zitsime zimapezeka m'ma lab ndi malo ofufuza padziko lonse lapansi. Amatha kusunga nthawi, khama, ndi malo osungira, koma dongosolo loyenera ndilofunika mukamaliza ntchito yanu.

Kuchokera pakuchulukira kosungirako, mpaka kusanganikirana kokulirapo, mbale zakuya zakuya ndizoyenera kuphatikizira chemistry ndi laibulale, kugonjetsedwa ndi mankhwala ambiri, zosungunulira, ndi zakumwa zoledzeretsa zomwe zimagwiritsidwa ntchito pophatikizana.

Zoyenera kusonkhanitsa zitsanzo, kukonzekera zitsanzo, ndi kusungirako kwanthawi yayitali (kapena kwakanthawi kochepa), mbale zakuya zachitsime ndi mateti osindikizira zitha kupititsa patsogolo kayendetsedwe ka ntchito, komanso mbale yakuzama yakumanja idzakuthandizaninso kupanga chidziwitso chapamwamba kwambiri cha ntchito wamba sayansi ya moyo (ndi kupitirira).