Momwe Mungalekere Kudontha Pamene Mumapha Zamadzimadzi Osakhazikika

Ndani sadziwa acetone, ethanol & co. kuyamba kutuluka kunjansonga ya pipettemwachindunji pambuyo chikhumbo? Mwinamwake, aliyense wa ife anakumanapo ndi izi. Maphikidwe achinsinsi monga "kugwira ntchito mwachangu momwe mungathere" pomwe "kuyika machubu pafupi kwambiri kuti asatayike ndi kutayika kwa mankhwala" ndizomwe mumachita tsiku ndi tsiku? Ngakhale madontho a mankhwala athamanga kwambiri, nthawi zambiri amaloledwa kuti pipetting si yolondola. Kusintha pang'ono chabe kwa njira zopangira mapaipi, komanso kusankha koyenera kwa mtundu wa pipette kungathandize kuthana ndi zovuta za tsiku ndi tsiku!

N'chifukwa chiyani ma pipette amatuluka?
Classic pipettes amayamba kudontha pamene pipette kosakhazikika zamadzimadzi chifukwa cha mpweya mkati mwa pipette. Izi otchedwa mpweya khushoni alipo pakati chitsanzo madzi ndi pisitoni mkati pipette. Monga momwe zimadziwikiratu, mpweya umasinthasintha ndipo umagwirizana ndi zochitika zakunja monga kutentha ndi kuthamanga kwa mpweya pokulitsa kapena kupondereza. Zamadzimadzi zimathanso kukhudzidwa ndi zinthu zakunja ndipo zimasanduka nthunzi mwachilengedwe popeza chinyezi cha mpweya chimakhala chochepa. Madzi osungunuka amasanduka nthunzi mofulumira kwambiri kuposa madzi. Pa pipetting, izo ukuphwera mu mpweya khushoni kukakamiza yotsirizira kuwonjezera ndi madzi mbamuikha kuchokera pipette nsonga ... pipette kukapanda kuleka.

Momwe mungapewere kuti zakumwa zisathe
Njira imodzi yochepetsera kapena kuyimitsa kudontha ndiyo kupeza chinyezi chambiri pamphuno ya mpweya. Izi zimachitika ndi pre-wetting thensonga ya pipettendi potero kukhutitsa mpweya khushoni. Pamene ntchito otsika kosakhazikika zamadzimadzi monga 70 % Mowa kapena 1 % acetone, aspirate ndi kugawira chitsanzo madzi osachepera 3 zina, pamaso aspirating chitsanzo buku mukufuna kusamutsa. Ngati ndende ya madzi kosakhazikika ndi apamwamba, bwerezani izi chisanadze kunyowetsa m'zinthu 5-8. Komabe, ndi kuchuluka kwambiri monga 100 % ethanol kapena chloroform, izi sizingakhale zokwanira. Ndi bwino kugwiritsa ntchito mtundu wina wa pipette: pipette yabwino yosamuka. Ma pipettes amagwiritsa ntchito nsonga zokhala ndi pistoni yophatikizika popanda mpweya. Chitsanzocho chikugwirizana mwachindunji ndi pisitoni ndipo palibe chiopsezo chodontha.

Khalani katswiri wa pipetting
Mutha kuwongolera kulondola kwanu mosavuta popaka zakumwa zosasunthika posankha njira yoyenera kapena kusintha chida chomwe mukugwiritsa ntchito. Kuphatikiza apo, mudzakulitsa chitetezo popewa kutayikira ndi kufewetsa kayendedwe kanu ka ntchito.