Momwe Mungasindikizire PCR Pulogalamu

Chiyambi

PCR Plates, lingaliro la labotale kwa zaka zambiri, likuchulukirachulukira mu malo omwe amapereka amagwira ntchito nthawi zambiri zomwe zimaperekedwa ndikugwiritsa ntchito makina mwazomwe zimachitika mkati mwazofuula. Kukwaniritsa zolinga izi pomwe pakusunga chivomerezo komanso kukhulupirika kwa kukhulupirika kumavuta. Chimodzi mwazinthu zomwe zolakwa zimatha kulowa ali ndi kusindikizidwa kwaPCR Plates, popanda luso lokwanira kuvomereza kusintha kwa zitsanzo, kusintha pH kenako kusokoneza enzymatic ntchito zosinthika, ndikuimba kuipitsidwa. Kuphunzira momwe mungasindikizirePCR mbalezimachotsa molondola zoopsa izi ndikuwonetsetsa zotsatira za kubereka.

Pezani Chisindikizo Choyenera cha PCR Lau Wanu

Pulogalamu ya mbale vs. filimu imasindikiza vs. lids
ChisotiNdi njira yabwino yosindikiza mbale yanu ndi chisindikizo cholimba, pomwe pomwe akukupatsani mwayi wosinthana mosavuta ndikusakanso mbaleyo momwe mungafunire popanda zinyalala zilizonse. Komabe, zisoti zimakhala ndi zovuta zingapo.

Choyamba, muyenera kugula chipewa chomwe chikugwirizana, chomwe chimawapangitsa iwo kukhala osasinthasintha. Muyenera kuonetsetsa kuti kapu yomwe mumasankha imakwanira mbale, yomwe imadalira wopanga, ndikusankha kuti muchepetse kapena kuthyolako potengera thermocler yomwe mukugwiritsa ntchito.

Kachiwiri, kugwiritsa ntchito zipilalazo kumbale kumatha kubwereza kwambiri komanso kumatha kukhala otopetsa, ndi chiopsezo chodetsa nkhawa ngati mungayike chipewa cholakwika.

Zisindikizo filimu khwalama mopepuka pochotsa ndikusintha, ndizosinthasintha momwe zimakhalira ndi mbale iliyonse ya PCR, osanena kuti wopanga ndi ndani. Amatha kudulidwa kukula, kuwapangitsa kukhala ogwira mtima kwambiri.

Njira ina ndi chivindikiro cha mbale. Izi zimapereka chitetezo chochepa kuti zisoti ndi zisindikizo, ndipo zimangogwiritsidwa ntchito ngati chivundikiro chochepa chopewa kuipitsidwa.

Makina owoneka bwino a V

Ngati mukufuna zotupa, zowoneka bwino kapenafilimu ya aluminiumKusindikiza mbale yanu imasankhidwa ndi mtundu wanu woyesera.Makanema owoneka bwinondizowonekera kulolani kuti mudziwe zitsanzo, pomwe pomwe amawateteza komanso kupewa kusinthasintha. Amathandizanso kwambiri pakuyesa kwa QPCR komwe kumaphatikizapo kupanga njira zodziwikiratu mwachindunji kuchokera pambale, mwina mungafunikire filimu yosindikiza yomwe ingatheke. Ndikofunikira kuwonetsetsa kuti chisindikizo kapena kapu yomwe mukugwiritsa ntchito ili ndi vuto lokwanira kuti mutsimikizire kuti kuwerengako ndi kolondola.

Makanema ovala ndi oyenera zitsanzo zilizonse zomwe zili zopepuka kapena ziyenera kusungidwa pansi pa 80 ° C. Pachifukwa ichi, zitsanzo zambiri zomwe zimapangidwira nthawi yayitali zimafunikira filimu yovala zojambula. Makanema okumba amathanso kuboola, omwe amathandiza kuti ayesere zitsime za munthu aliyense payekha, kapena kusamutsa zitsanzo ndi singano. Izi zitha kuchitika pamanja kapena gawo la nsanja ya Robotic.

Lingaliraninso kuti zinthu zomwe zimaphatikizira acid, zitsulo kapena ma sol sol zimafunikira chidindo chomwe chitha kupirira.

Zomatira vs. filimu yolimba
Zisindikizo Zomatandi ntchito yolunjika kwambiri komanso yosavuta. Zomwe mukufunikira ndi za wogwiritsa ntchito kuyika chidindo kupita ku mbaleyo, ndikugwiritsa ntchito chida chosavuta cholowerera pansi ndikupanga Chisindikizo cholimba.

Zisindikizo za kutentha zimakhala zapamwamba kwambiri, kupereka chisindikizo chokhalitsa chomwe chachepetsa mizu yosinthira anthu poyerekeza ndi chidindo chomatira. Njira iyi ndi yoyenera ngati mukufuna kusunga zitsanzo munthawi yayitali, ngakhale izi zimapezeka ndi zofunikanso kwa zida zosindikizira.

Momwe Mungasindikizire PCR Pulogalamu

Njira yosindikiza

Zomatira

1. Onetsetsani kuti mukugwira ntchito pazinthu zokhazikika komanso zokhazikika

2. Chotsani filimuyi kuchokera ku malo ake, ndikuchotsa

3. Ikani chidindo mosamala pambale, pomwe mukuwonetsetsa kuti zitsime zonse zaphimbidwa

4. Gwiritsani ntchito cholembera chogwiritsira ntchito kuweta mbale. Yambani kuchokera kumapeto limodzi ndikugwira ntchito kwa winayo, kukanikiza motero

5. Bwerezaninso nthawi zambiri izi

6. Yendetsani wofunsira wanu kuzungulira zitsime zakunja, kuti muwonetsetse kuti izi zikusindikizidwa bwino.

Kutentha Zisindikizo

Kutentha kumagwira ntchito ndikusungunula filimuyo kumbali ya chitsime chilichonse, mothandizidwa ndi wobzala mbale. Kuti mugwiritse ntchito kutentha kwamoto, onani malangizo omwe opanga zida. Onetsetsani kuti wopanga zomwe mumapanga kuti mupange zida zanu kukhala zodziwika bwino, chifukwa ndizofunikira kwambiri kuti chisindikizo ndichoyenera, chothandiza komanso chamadzi.

Pulogalamu Yapamwamba Yapamwamba

a. Mukamagwiritsa ntchito chidindo, pitani mbali zonse zopingasa ndi zowongoka kuti zitsimikizire chidindo choyenera

b. Nthawi zonse pamakhala chizolowezi chothamangitsa chilichonse chomwe mukuchita, ndipo izi sizosiyana ndi kusindikizidwa pagalimoto. Yesani ndi mbale yopanda kanthu musanagwiritse ntchito imodzi ndi zitsanzo.

c. Mukamayesa, tengani chisindikizo ndikuyang'ana kuti muone kuti zomata zinkatsika moyenera, popanda mipata. Pali choyimira chowoneka cha izi mu chikalata choyambirira. Ngati simunasindikize mbale moyenera, mukachotsa chidindo padzakhala mipata pomwe zomatira sizimalumikizana bwino ndi mbale.

d. Potumiza ndi kunyamula zitsanzo, mungaone kuti ndizothandiza kuyika chidindo cha pulasitiki pamwamba pa chisindikizo chowonjezera kuti chitetezeke (makamaka pakuboola).

e. Nthawi zonse onetsetsani kuti mulibe makwiya kapena makwinya akamagwiritsa ntchito filimuyi - izi zimapangitsa kutaya ndikusintha