Momwe Mungasindikize Plate ya PCR

Mawu Oyamba

Zithunzi za PCR, chomwe chili chodziwika bwino mu labotale kwa zaka zambiri, chikuchulukirachulukira m'masiku ano pomwe ma laboratories amakulitsa ntchito zawo ndikugwiritsa ntchito makina opangira okha mkati mwamayendedwe awo. Kukwaniritsa zolingazi ndikusunga kulondola ndi kukhulupirika kwa zoyeserera kungakhale kovuta. Mmodzi mwa madera wamba kumene zolakwa akhoza kukwawira ndi ndi kusindikiza kwaZithunzi za PCR, ndi njira yosauka yomwe imalola kuti zitsanzo zikhale nthunzi, kusintha pH motero kusokoneza ntchito za enzymatic, ndi kuyitanitsa kuipitsidwa. Kuphunzira kusindikiza aChithunzi cha PCRmolondola amachotsa zoopsazi ndikuwonetsetsa zotsatira zobwerezedwa.

Pezani Chisindikizo Choyenera cha PCR Plate yanu

Plate Caps vs. Film Seals vs. Lids
KapuNdi njira yabwino yosindikizira mbale yanu ndi chosindikizira cholimba, ndikukupatsani mwayi woti mumasule mosavuta ndikusindikizanso mbale momwe mungafunire popanda kutaya chilichonse. Komabe, makapu ali ndi zovuta zingapo.

Choyamba, muyenera kugula kapu yeniyeni yomwe imagwirizana, zomwe zimapangitsa kuti zisasunthike. Muyenera kuwonetsetsa kuti chipewa chomwe mwasankha chikugwirizana ndi mbaleyo, yomwe imadalira wopanga wake, ndikusankha zokhala ndi domed kapena zosalala kutengera thermocycler yomwe mukugwiritsa ntchito.

Kachiwiri, kugwiritsa ntchito zipewa ku mbale kumatha kukhala kobwerezabwereza komanso kotopetsa, ndi chiwopsezo cha kuipitsidwa ngati mutayika chipewa cholakwika pachitsime cholakwika.

Ngakhale zisindikizo zamakanema sizimasinthasintha pochotsa ndikusintha, zimakhala zosunthika kwambiri chifukwa zimakwanira mbale yamtundu uliwonse wa PCR, mosasamala kanthu za wopanga. Amatha kudulidwa kukula kwake, kuwapanga kukhala othandiza kwambiri.

Njira ina ndi chivindikiro cha mbale. Izi zimapereka chitetezo chocheperako kuposa zipewa ndi zosindikizira, ndipo zimagwiritsidwa ntchito makamaka pakuphimba kwakanthawi kochepa kuti zisawonongeke.

Optical vs. Foil Film Zisindikizo

Kaya mukufuna chosindikizira chowoneka bwino, chomveka bwino kapena chosindikizirafilimu ya aluminium zojambulazokusindikiza mbale yanu imasankhidwa ndi mtundu wanu woyesera.Mafilimu osindikizira a Opticalndi zowonekera kukulolani kuti muwone zitsanzo, kwinaku mukuziteteza ndikupewa kutuluka kwa nthunzi. Zimakhalanso zothandiza kwambiri pakuyesa kwa qPCR komwe kumaphatikizapo kupanga miyeso yolondola kwambiri ya fluorescence mwachindunji kuchokera mu mbale, momwemo mudzafunika filimu yosindikizira yomwe imasefa ngati fulorosisi pang'ono momwe mungathere. Ndikofunikira kuwonetsetsa kuti chisindikizo kapena kapu yomwe mukugwiritsa ntchito ili ndi mawonekedwe owoneka bwino kuti muwonetsetse kuti zowerengera ndi zolondola.

Mafilimu opangidwa ndi zojambulazo ndi oyenera kwa zitsanzo zilizonse zomwe zimakhala zopepuka kapena ziyenera kusungidwa pansi pa 80 ° C. Pachifukwa ichi, zitsanzo zambiri zomwe zimasungidwa kwa nthawi yayitali zimafuna filimu ya zojambulazo. Mafilimu a zojambulazo amathanso kuboola, zomwe zimakhala zothandiza pofufuza zitsime zapayekha, kapena kusamutsa zitsanzo ndi singano. Izi zitha kuchitika pamanja kapena ngati gawo la nsanja ya robotic.

Ganiziraninso kuti zinthu zaukali zomwe zimaphatikizapo ma acid, mabasi kapena zosungunulira zimafuna chisindikizo chomwe chitha kupirira, ndiye kuti chosindikiziracho chimakhala choyenera kwambiri.

Filimu Yomatira motsutsana ndi Kutentha Kwambiri
Zisindikizo za filimu zomatirandizowongoka kwambiri komanso zosavuta kugwiritsa ntchito. Zomwe mukufunikira ndi kuti wogwiritsa ntchito agwiritse ntchito chisindikizo pa mbale, ndikugwiritsa ntchito chida chosavuta chogwiritsira ntchito kuti asindikize ndi kupanga chisindikizo cholimba.

Zisindikizo za kutentha zimakhala zapamwamba kwambiri, zomwe zimapereka chisindikizo chokhalitsa chomwe chachepetsa kuchepa kwa mpweya poyerekeza ndi chisindikizo chokhazikika. Njira iyi ndi yoyenera ngati mukufuna kusunga zitsanzo kwa nthawi yayitali, ngakhale izi zimabwera ndi zofunikira zowonjezera pazida zosindikizira mbale.

Momwe Mungasindikize Plate ya PCR

Njira Yosindikizira Plate

Zodzimatira

1. Onetsetsani kuti mukugwira ntchito pamalo osalala komanso okhazikika

2. Chotsani filimuyo m'matumba ake, ndikuchotsani chothandizira

3. Ikani chosindikiziracho mosamala pa mbale, ndikuwonetsetsa kuti zitsime zonse zakutidwa

4. Gwiritsani ntchito chida chogwiritsira ntchito kuti mugwiritse ntchito kukakamiza pa mbale. Yambani kuchokera kumalekezero ena ndikugwira njira yanu mpaka ina, kukanikiza mofanana

5. Bwerezani izi kangapo

6. Thamangani pulogalamu yanu kuzungulira zitsime zakunja, kuti muwonetsetse kuti izi nazonso zasindikizidwa bwino.

Kutentha Zisindikizo

Zisindikizo za kutentha zimagwira ntchito posungunula filimuyo kumphepete mwa chitsime chilichonse, mothandizidwa ndi chosindikizira mbale. Kuti mugwiritse ntchito chosindikizira kutentha, tchulani malangizo operekedwa ndi wopanga zida. Onetsetsani kuti wopanga yemwe mumatengera zida zanu ndi wodalirika, chifukwa ndikofunikira kwambiri kuti chisindikizocho ndi choyenera, chogwira ntchito komanso chopanda madzi.

Malangizo Apamwamba Osindikiza Plate

a. Mukakakamiza chisindikizo, pitani mbali zonse zopingasa komanso zoyima kuti muwonetsetse kuti chisindikizocho chili choyenera.

b. Nthawi zonse ndi bwino kuyesa kuyesa chilichonse chomwe mukuchita, ndipo izi sizosiyana ndi kusindikiza mbale. Yesani ndi mbale yopanda kanthu musanagwiritse ntchito yokhala ndi zitsanzo.

c. Poyesa, chotsani chisindikizo ndikuyang'ana kuti muwone kuti zomatirazo zakhazikika bwino, popanda mipata. Pali chithunzithunzi cha izi m'chikalata choyambirira. Ngati simunasindikize mbaleyo bwino, mukachotsa chisindikizocho padzakhala mipata pomwe zomatira sizinapange mgwirizano wokwanira ku mbale.

d. Potumiza ndi kunyamula zitsanzo, mutha kuwona kuti ndizothandiza kuyika chisindikizo cha pulasitiki pamwamba pa chosindikiziracho kuti mutetezedwe kwambiri (makamaka pakuboola).

e. Nthawi zonse onetsetsani kuti palibe mabampu kapena makwinya mukamagwiritsa ntchito filimuyo - izi zipangitsa kutayikira ndi mpweya.