Momwe mungagwiritsire ntchito ziphuphu zazing'ono ndi machipi oyenda pamanja

Kuyang'ana kwambiri kumayikidwa pakuchepetsa magwero ndi ndalama, kuchuluka kwa mavoling'ono kuli kofunikira kwambiri, mwachitsanzo, pokonzekera pcr ambuye kapena enzyme. Koma popereka mavoliyulosi yaying'ono kuchokera ku 0,2 - 5 μl imakhazikitsa zovuta zatsopano zokupatsani kulondola komanso kulondola. Mfundo zotsatirazi ndizofunikira:

1. Pipette ndi kukula kwa nsonga: Nthawi zonse sankhani pipette ndi voliyumu yotsika kwambiri komanso nsonga yaying'ono kwambiri kuti khungu la ndege lithere. Pakato 1 μl, sankhani 0,25 - 2.2 μl pipette ndi nsonga yofananira osati pipette ya 1 - 10 μl.
2. Kukonza ndi kukonza: ndikofunikira kuti ma pipette anu amadziwika bwino ndikusungidwa. Zosintha zazing'ono ndi ziwalo zosweka pa pipette zimayambitsa kuwonjezeka kwakukulu munthawi mwamwayi komanso mwachisawawa. Katswiri Malinga ndi ISO 8655 iyenera kuchitidwa kamodzi pachaka.
3. Mapaipesi okusakanikirana: Onani ngati muli ndi ma pipette yolimba ndi voliyumu yotsika mu labu yanu. Mwambiri, kugwiritsa ntchito pipette yamtunduwu kumabweretsa zotsatira zabwino kwambiri malinga ndi kulondola komanso molondola kuposa ma pichet a mlengalenga.
4. Yesani kugwiritsa ntchito mavoliyumu akuluakulu: Mutha kuganizira kuchepetsera zitsanzo zanu pa pipette zokulirapo ndi kuchuluka komweko. Izi zimatha kuchepetsa zolakwika zachipongwe ndi mavidiyo ang'onoang'ono kwambiri.

Kuphatikiza pa chida chabwino, wofufuzayo ayenera kukhala ndi njira yabwino kwambiri yopumira. Samalani kwambiri ndi izi:

1. Kuphatikizika: Musamapanikitse pipette pamutuwu chifukwa izi zitha kuwononga mbali yabwino ya nsonga imapangitsa kuti mtengowo uzibwezeredwa kapena kuwononga orifice. Ingogwirani ntchito yowala pang'ono mukamagwirizanitsa nsonga ndikugwiritsa ntchito pipette ndi cue.
2. Atanyamula pipette: Musagwire pipette m'manja mwanu mukudikirira centrifuge, mkati mwa pipette idzakulitsa kupatuka kuchokera ku mapangidwe ochokera ku makhothi.
3. Kunyowa: Kutentha kwa mpweya mkati mwa nsonga ndi pipitte kumakonzekeretsa nsongayo ndikupeputsa kusinthaku mukamatsata voliyumu yosamutsa.
4. Kukhumba Kufukula: Izi ndizofunikira kwambiri pogwirira maphunziro ang'onoang'ono kuti tipewe mphamvu ya capillary yomwe imachitika pomwe pipette imachitika pakona.
5. Kuzama kwamizidwa: kumiza mfundo zochepa momwe mungathere kuteteza madzi kulowa nsonga chifukwa cha capillary zotsatira zake. Lamulo la chala: Zocheperako nsonga ndi voliyumu, kutsikira kumizidwa. Tikupangira gawo lalitali 2 mm pamene mapaupi ang'onoang'ono.
6. Kuyika 45 ° Angle: Kutulutsa koyenera kwa madzi kumatsimikizika pomwe pipette imachitika pa 45 °.
7. Kulumikizana ndi Vossel Khoma kapena Madzimadzi: Ma voliyumu ang'onoang'ono amatha kuperekedwera bwino pomwe nsongayo imasungidwa khoma la chotengera, kapena kumizidwa mu madzi. Ngakhale dontho lomaliza kuchokera pa nsonga likhoza kuperekedwa molondola.
8. Kuphulika: Kuphulika ndi kuvomerezedwa pambuyo popereka mavoliyumu otsika kuti atulutse ngakhale dontho lomaliza lamadzi lomwe lili ndi nsonga. Kuphulika kuyenera kuchitika motsutsana ndi khoma la chotengera. Musamale kuti musabweretse thovu kulowa mu chitsanzo mukamachita zowombera pamadzimadzi.