Momwe Mungapangire Mabuku Ang'onoang'ono Okhala Ndi Ma Pipettes A Pamanja

Pamene chidwi chikuyikidwa pa kuchepetsa ma reagents ndi ndalama, mavoliyumu ang'onoang'ono akufunika kwambiri, mwachitsanzo, pokonzekera PCR Mastermix kapena machitidwe a enzyme. Koma kuyika mapaipi ang'onoang'ono kuchokera ku 0.2 - 5 µL kumabweretsa zovuta zatsopano pakulondola kwa mapaipi komanso kulondola. Mfundo zotsatirazi ndi zofunika:

1. Pipette ndi kukula kwa nsonga: Nthawi zonse sankhani pipette yokhala ndi voliyumu yotsika kwambiri yotheka komanso nsonga yaying'ono kwambiri kuti koshini ya mpweya ikhale yaying'ono momwe mungathere. Mukamapaka 1 µL mwachitsanzo, sankhani pipette ya 0.25 - 2.5 µL ndi nsonga yofananira osati 1 - 10 µL pipette.
2. Kuwongolera ndi kukonza: Ndikofunikira kuti ma pipette anu awonedwe bwino ndikusamalidwa. Kusintha kwakung'ono ndi magawo osweka pa pipette kumabweretsa kuwonjezeka kwakukulu kwa zolakwika mwadongosolo komanso mwachisawawa. Kuwongolera molingana ndi ISO 8655 kuyenera kuchitika kamodzi pachaka.
3. Ma pipette abwino osamutsidwa: Onani ngati muli ndi pipette yabwino yosamutsidwa yokhala ndi kuchuluka kwa voliyumu yotsika mu labu yanu. Kawirikawiri, kugwiritsa ntchito mtundu uwu wa pipette kumabweretsa zotsatira zabwino za pipetting ponena za kulondola ndi kulondola kusiyana ndi ma pipette apamwamba a mpweya.
4. Yesani kugwiritsa ntchito mavoliyumu okulirapo: Mutha kulingalira kutsitsa zitsanzo zanu kukhala ma volumes akulu ndi kuchuluka komweko pomaliza. Izi zitha kuchepetsa zolakwika za pipetting ndi ma voliyumu ochepa kwambiri.

Kuphatikiza pa chida chabwino, wofufuzayo ayenera kukhala ndi njira yabwino kwambiri yopangira mapaipi. Samalani kwambiri pa izi:

1. Thandizo laupangiri: Osapanikizira pipette kunsonga chifukwa izi zitha kuwononga nsonga yabwino kupangitsa kuti mtengo wamadzimadzi uwolokedwenso kapena kuwononga potuluka. Ingogwiritsani ntchito zopepuka zopepuka mukamangirira nsonga ndikugwiritsa ntchito pipette yokhala ndi nsonga yodzaza masika.
2. Kugwira pipette: Musagwire pipette m'manja mwanu pamene mukudikirira centrifuge, cycler, etc. Mkati mwa pipette idzatenthetsa ndikutsogolera mpweya wa mpweya kuti uwonjezeke, zomwe zimapangitsa kuti zikhale zosiyana ndi voliyumu yomwe imayikidwa pamene pipetting.
3. Pre-kunyowetsa: The humidification wa mpweya mkati nsonga ndi pipette kukonzekera nsonga chitsanzo ndi kupewa evaporation pamene aspirate kusamutsa voliyumu.
4. Vertical aspiration: Izi ndizofunikira kwambiri pogwira ma voliyumu ang'onoang'ono kuti mupewe mphamvu ya capillary yomwe imachitika pamene pipette imayikidwa pamtunda.
5. Kuzama kwa kumizidwa: Mizidwa nsonga pang'ono momwe mungathere kuti madzi asalowe kunsonga chifukwa cha mphamvu ya capillary. Lamulo la chala chachikulu: Kuchepa kwa nsonga ndi voliyumu, kumachepetsa kuya kwa kumizidwa. Mpofunika munthu pazipita 2 mm pamene pipetting mabuku ang'onoang'ono.
6. Kupereka pa ngodya ya 45 °: Kutuluka bwino kwamadzimadzi kumatsimikiziridwa pamene pipette imagwira pa ngodya ya 45 °.
7. Kulumikizana ndi khoma la chotengera kapena pamwamba pamadzi: Ma voliyumu ang'onoang'ono amatha kuperekedwa moyenera pamene nsonga ikanikiza khoma la chombo, kapena kumizidwa mumadzimadzi. Ngakhale dontho lomaliza kuchokera kunsonga likhoza kuperekedwa molondola.
8. Kuphulitsa: Kuphulitsa kumafunika mutapereka ma volume ochepa kuti mutulutse ngakhale dontho lomaliza lamadzimadzi lomwe lili pansonga. Kuwombera kuyeneranso kuchitidwa motsutsana ndi khoma la chombo. Samalani kuti musabweretse thovu la mpweya mu chitsanzo pamene mukuphulitsa pamwamba pa madzi.