Makina opangira mapaipindi imodzi mwa njira zothandiza kwambiri zochepetsera zolakwika za anthu, kuwonjezera kulondola ndi kulondola, ndikufulumizitsa kayendedwe ka labu. Komabe, kusankha pazigawo "zomwe muyenera kukhala nazo" kuti muzitha kuyendetsa bwino ntchito yamadzimadzi zimatengera zolinga zanu ndi ntchito. Nkhaniyi ikufotokoza zina mwa mfundo zofunika kuziganizira posankha nsanja yamadzi yogwirira ntchito ya labotale yanu.
Kupanga mapaipi ndi gawo lofunikira pakuwongolera kayendedwe ka labotale, kuthandizira kuchulukitsa kuberekana, kukulitsa kutulutsa, ndikuchepetsa zolakwika. Ma laboratories amadalira matekinoloje ogwiritsira ntchito madzi opangira zinthu zosiyanasiyana, kuphatikizapo kukonzekera zitsanzo, kuchotsa DNA, kuyesa pogwiritsa ntchito ma cell, ndi ELISAs. Mapulatifomuwa ndi ndalama zanthawi yayitali ndipo sayenera kusankhidwa motengera zomwe masiku ano akufuna, komanso zomwe zingachitike m'tsogolo labu. Izi zidzatsimikizira kuti nsanja yolondola imasankhidwa, ndipo ikhoza kutumikira bwino labotale kwa zaka zambiri zikubwerazi.
Masitepe oyamba
Musanapange zisankho zilizonse, yang'anani bwino njira zomwe zikuyenera kukhala makina:
Kodi mukuyamba ndi ndondomeko yamphamvu?
Makina ogwiritsira ntchito amadzimadzi amatha kusintha kwambiri kayendedwe ka ntchito, koma sangathe kukonza chiyeso chomwe sichikugwira ntchito kale. Gwirani ntchito yanu m'masitepe apawokha, ndipo ganizirani zomwe zingakhudze chilichonse pamayendedwe onse. Mwachitsanzo, kutenga kuyesa kuchokera pamapaipi opangidwa pamanja, machubu opangidwa ndi machubu kupita kumayendedwe okhazikika, okwera kwambiri, otengera mbale kumatanthauza kuti zitsanzo ndi ma reagents azikhala pa sitimayo kwa nthawi yayitali. Kodi izi zingakhudze bwanji kukhulupirika kwa zitsanzo zanu ndi ma reagents?
Zosowa zanu zidzasintha bwanji?
Kuti musunge ndalama, zitha kukhala zokopa kuyika ndalama munjira yomwe imangokwaniritsa zosowa za labu yanu, koma pakapita nthawi mutha kutaya. Ganizirani zomwe zili zofunika, ndi zomwe zingakhale zabwino kukhala nazo. Dongosolo labwino lamadzimadzi lamadzimadzi liyenera kusinthidwanso kuti mutha kugwiritsa ntchito zatsopano ndi mayendedwe a ntchito ngati zosowa zikusintha. Ndi makina osinthika, osinthika, zinthu zambiri zamayendedwe anu apano zitha kusinthidwanso ndikusinthidwa.
Kodi pali yankho la pashelufu lomwe limakwaniritsa zosowa zanu?
Malo ena apadera ogwirira ntchito adakonzedwa kuti azigwiritsidwa ntchito mwapadera ndi ma protocol otsimikiziridwa, monga kuchotsa DNA, kukonzekera zitsanzo, ndi chikhalidwe cha ma cell. Izi zitha kufewetsa njira yanu yosankha, ndikukupatsani gawo lofunikira la "core" kuti muphatikizidwe mudongosolo lalikulu mtsogolo. Mayankho akunja a alumali opangidwa ndikuphatikizana m'tsogolo ndi kusinthasintha m'malingaliro ndi abwino kuposa nsanja zosasinthika, "zotsekedwa".
Muli ndi malo ochuluka bwanji, ndipo mukuwagwiritsa ntchito moyenera?
Malo nthawi zambiri amakhala chinthu chamtengo wapatali. Makina ambiri ogwiritsira ntchito madzi tsopano ndi ogwiritsa ntchito ambiri, zomwe zachulukitsa kufunikira kwa kusinthasintha komanso kugwiritsa ntchito mwanzeru malo. Lingalirani kusankha nsanja yodzichitira yomwe ingathe kupeza malo pansi pa tebulo logwirira ntchito kuti mufike, mwachitsanzo, zida zowonjezera zowunikira kapena zitsanzo zokonzekera, ndi zina.
Ndikophweka bwanji kukonza ndi kutumikira?
Musanyalanyaze kusamalira ndi kusamalira. Kupezeka mosavuta ndi akatswiri kumatha kuchepetsa nthawi yopumira komanso zosokoneza pakuyenda kwanu.
Kusankha zida zoyenera
Kaya mukugwira ntchito mu genomics, cell biology, kupezeka kwa mankhwala, kufufuza mamolekyulu, kapena china chake chosiyana, njira yoyenera yoyendetsera madzi imatha kupangitsa moyo wanu kukhala wosavuta. Zofunikira zofunika ndizo:
Mpweya kapena madzi kusamuka mapaipi?
Kusamuka kwa mpweya ndikoyenera kugawira pamitundu yayikulu, kuyambira 0,5 mpaka 1,000 μL. Ngakhale zimangogwirizana ndi maupangiri otayidwa, izi zimawonjezera liwiro komanso zokolola pochotsa njira zowonjezera zomwe zimagwirizanitsidwa ndi kusuntha kwamadzimadzi posintha zamadzimadzi kapena kutulutsa makina. Zimachepetsanso chiopsezo cha kuipitsidwa kwapakati ndikupereka njira yotetezeka yogwiritsira ntchito zipangizo zowononga ma radioactive kapena biohazardous.
Kusamuka kwamadzi kumagwirizana ndi maupangiri osasunthika komanso otayidwa, ndipo ndiye ukadaulo womwe umakondedwa wa ma voliyumu ambiri osakwana 5 μL. Nsonga zachitsulo zosasunthika zochapitsidwa ndizoyenera kugwiritsa ntchito pomwe machubu amafunikira kuboola kapena kuponderezedwa kwapaipi kumafunika. Kuti muzitha kusinthasintha, ganizirani dongosolo lomwe limaphatikizapo kusamuka kwa mpweya ndi madzi.
Ndi ma voliyumu ndi mawonekedwe otani omwe mumagwira nawo ntchito?
Onetsetsani kuti nsanja imatha kugwiritsa ntchito ma voliyumu ofunikira ndi ma labware (machubu ndi mbale) zomwe zimagwiritsidwa ntchito mu labu yanu. Ganiziraninso ngati ma automation angalole kuti zitsanzo zing'onozing'ono ndi ma reagents agwiritsidwe ntchito, zomwe zingachepetse mtengo.
Ndi mikono iti ya mapaipi yomwe muyenera kusankha?
Mitundu ikuluikulu ndi 1) ma pipette osinthasintha-nthawi zambiri 1- mpaka 8-channel-omwe amatha kugwiritsira ntchito machubu, mbale, ndi mitundu ina yambiri ya labware; ndi 2) mikono yambirimbiri yopangidwa kuti igawidwe m'mbale zokhala ndi zitsime zingapo. Machitidwe amakono amalola kuti mitu ya pipetting kapena ma adapter plates asinthidwe "pa ntchentche" -kusankha mwanzeru kwa ma protocol omwe amagwiritsa ntchito zipangizo zosiyanasiyana, monga singano zosasunthika, nsonga zowonongeka, zida za pini zotsika, ndi zina zotero.
Kodi mukufunikira zida za roboticzaowonjezera kusinthasintha?
Mikono ya robotic gripper imapereka kusinthasintha kwakukulu posuntha ma labware kuzungulira malo ogwirira ntchito. Mikono ya robotic yomwe imatha kusintha "zala" zawo mwachangu imatsimikizira kusinthasintha kwakukulu komanso kugwira kotetezeka kwa machubu ndi mbale zonse.
Ndi nsonga yamtundu wanji ya pipette yomwe ingawonjezere kuberekana?
Ubwino wamalangizo ndiwothandiza kwambiri pakubalanso ndipo utha kupanga kapena kusokoneza magwiridwe antchito. Malangizo otayika nthawi zambiri amawonedwa ngati njira yabwino kwambiri yochotsera kuipitsidwa pakati pa zitsanzo zamoyo. Ogulitsa ena tsopano amaperekanso malangizo apadera otsika kwambiri ovomerezeka kuti agawidwe odalirika pamilingo ya microliter kapena submicroliter yofunikira pakugwiritsa ntchito monga assay miniaturization. Ganizirani zogulira maupangiri a pipette kwa ogulitsa makina kuti muwonetsetse kuti mumapeza zotsatira zodalirika.
Zida zogwiritsira ntchito malangizo osasunthika zingakhale ndi ubwino wokhudzana ndi mtengo wa ntchito. Singano zachitsulo zokhazikika zimatha kufika pansi paziwiya zakuya kuposa nsonga zotayidwa, komanso kuboola septa. Malo ochapira okonzedwa bwino amachepetsa chiopsezo choyipitsidwa ndi khwekhweli.
Kodi mukufuna malangizo omwe ali otsimikizika kuti ndi osabereka?
Kuti muchepetse kuipitsidwa, gwiritsani ntchito zinthu zodyedwa zomwe zalembedwa kuti "zosabala." Izi zimapangidwa pansi pamikhalidwe yovuta kwambiri ndipo zimagwirizana ndi zonyamula ndi zoyendera zomwe zimatsimikizira kusabereka mpaka ku benchi ya labu. Zogulitsa zotchedwa "presterile" zimakhala zosabala zikachoka kwa wopanga, koma zimakumana ndi mwayi wambiri woipitsidwa pambuyo pake.
Mapulogalamu amafunikira
Mapulogalamu amapereka mawonekedwe ndi munthu amene akukhazikitsa ndikugwiritsa ntchito chidacho, ndipo mapangidwe ake adzatsimikizira momwe zimakhalira zosavuta kupanga ndi kuyanjana ndi dongosolo kuti zikhazikitse kayendedwe ka ntchito, kukhazikitsa magawo a ndondomeko, ndi kupanga zosankha zogwiritsira ntchito deta. Zimakhalanso ndi zotsatira zachindunji pa kuchuluka kwa maphunziro omwe amafunikira kuti agwiritse ntchito dongosololi molimba mtima. Pokhapokha mutakhala ndi katswiri wa mapulogalamu m'nyumba, mapulogalamu opangidwa molakwika, ngakhale atakhala amphamvu bwanji, akhoza kukusiyani kuti mudalira wogulitsa kapena katswiri wakunja kuti apange ma protocol ogwirizana, kuthetsa mavuto, ndi kupanga ngakhale kusintha kosavuta kwa mapulogalamu. M'ma laboratories ambiri, wogwiritsa ntchito makina si katswiri wamapulogalamu, ndipo magulu ambiri a IT sangalowe nawo mwachindunji ndi pulogalamu yowongolera zida. Zotsatira zake, mungafunike kudikirira kuti alangizi akunja apezeke, zomwe zimalepheretsa kwambiri zokolola ndikuyika nthawi ya polojekiti pachiwopsezo.
Mfundo zofunika kuziganizira
Mafunso ofunikira omwe muyenera kufunsa mukawunika pulogalamu yamagetsi yamadzimadzi ndi awa:
- Kodi ogwiritsa ntchito angagwirizane ndi chotchinga chogwira ntchito tsiku lililonse?
- Kodi wogulitsa ali ndi laibulale yama protocol omwe alipo kuti achepetse mapulogalamu?
- Kodi pali kuthekera kotani kophatikiza mapulogalamu pazida za anthu ena?
- Kodi laibulale yoyendetsa zida zoperekedwa ndi wogulitsa ndi yotani?
- Kodi wogulitsa amakumana ndi kulumikizana kwa LIMS?
- Kodi mungakhale omasuka kupanga dongosolo nokha?
- Kodi ndizosavuta bwanji kwa ogwiritsa ntchito kukhazikitsa mapulogalamu awo popanda ukadaulo wamapulogalamu?
- Ndi zinthu ziti, monga maupangiri ojambulira makonda - zomwe mukufuna, ndipo zilipo?
- Kodi n'kosavuta kukonzanso pulogalamuyo pamene dongosolo lakonzedwanso?
- Kodi wogulitsa angathandize kuonetsetsa chitetezo cha cyber?
Chitsanzo cha traceability
Kutsatiridwa kwathunthu kwachitsanzo kungakhale kofunikira pakutsata miyezo ndi malangizo abwino. Kulemba zilembo za barcode, limodzi ndi mapulogalamu oyenerera, kumathandizira kutsata zitsanzo zonse ndi zinthu zomwe zimagwiritsidwa ntchito, ndipo zitha kupewa kutayika kwa kutsata. Mayankho odzilembera okha ndi kutsatira njira angathenso:
- Sonyezani malo a labware pa sitimayo ndi malo osungira
- Onetsetsani kuti zilembo za barcode zayikidwa bwino ndipo zitha kuwerengedwa bwino
- Limbikitsani kuwerenga kwa barcode ndikusankha zitsanzo, ndikuwongolera kuphatikiza kwapakati ndi LIMS.
Njira yoti alowererepo
Zolakwa zimapangidwa mosavuta, koma sizophweka nthawi zonse kukonza. Makina ambiri odzichitira alibe ntchito za "kuyamba/kuyimitsa" kapena "kusintha", zomwe zingatanthauze kuyambitsanso pulogalamu ngati mulowetsa china chake molakwika kapena muyenera kuyimitsa kaye. Yang'anani makina anzeru odzipangira okha omwe amatha kuzindikira, kumvetsetsa, kupereka lipoti, ndikuchira ku cholakwika, ndikuyambitsa / kuyimitsa magwiridwe antchito kuti alole kulumikizana kotetezeka komanso kosavuta kwa wogwiritsa ntchito ndi gawo lachidacho panthawi yothamanga.
Chidule
Kugwiritsa ntchito madzi pawokha kumatha kuthetsa ntchito zambiri zotopetsa, kukulitsa zokolola ndikumasula nthawi yofunika kwambiri pantchito yofunika kwambiri - koma pokhapokha mutagwiritsa ntchito njira zoyenera. Kuganizira mozama mfundo zimene takambirana m’nkhani ino kungathandize kuti ma laboratories asankhe mwanzeru, kuti athe kupeza phindu pogwiritsa ntchito makina amadzimadzi ndiponso kuti moyo ukhale wosavuta ndiponso wopindulitsa.
Nthawi yotumiza: May-10-2022