Machubu a PCR Apamwamba: 0.1mL White 8-Strip PCR Tubes kwa Zotsatira Zabwino Kwambiri za PCR

Pankhani ya biology ya mamolekyulu, Polymerase Chain Reaction (PCR) ndi njira yapangodya yomwe yasintha momwe timakulitsira ndikusanthula magawo ena a DNA. Kupeza zotsatira zabwino za PCR kumafunikira osati zida zodziwika bwino zokha komanso zopangira zida komanso zida zapamwamba kwambiri, makamaka machubu a PCR. Lero, ndine wokondwa kukudziwitsaniACE's 0.1mL White 8-Strip PCR Tubes, opangidwa kuti apititse patsogolo zoyeserera zanu za PCR ndikuwonetsetsa kuti ndizolondola komanso zochulukira. Tiyeni tilowe muzinthu ndi zopindulitsa zomwe zimapangitsa kuti machubu awa akhale chinthu chofunikira kwambiri pa kafukufuku wanu kapena labu yowunikira.

Chifukwa Chiyani Musankhe Machubu a ACE a 0.1mL White 8-Strip PCR?

1.Ubwino Wosafanana ndi Kukhazikika

Ku ACE, timanyadira kuti ndife otsogola ogulitsa zinthu zapulasitiki zapamwamba kwambiri zachipatala ndi labotale. Machubu athu a 0.1mL White 8-Strip PCR amapangidwa pogwiritsa ntchito ukadaulo wopangira jakisoni wamakono komanso njira zowongolera bwino. Izi zimawonetsetsa kuti chubu chilichonse chikukwaniritsa miyezo yapamwamba kwambiri yolondola, yofanana, komanso yokhazikika, yofunikira kuti PCR igwire ntchito mosasinthasintha pazoyeserera zanu zonse.

2.Zokongoletsedwa ndi ma Protocol a PCR

Mapangidwe a machubu athu a mizere 8 amapangidwa kuti agwirizane bwino ndi ma cyclers otenthetsera, kuchepetsa mipata yomwe ingayambitse kutentha ndi kuzizira kosiyana. Mphamvu ya 0.1mL ndi yabwino kwa mapulogalamu osiyanasiyana a PCR, kuyambira kukulitsa kwa DNA mpaka kumachitidwe ovuta kwambiri a multiplex, kuonetsetsa kuti mutha kupeza zotsatira zolimba komanso zobereka nthawi zonse.

3.Mtundu Woyera Wothandizira Kuwoneka Bwino

Mtundu woyera wa machubu a PCRwa umapereka mawonekedwe owoneka bwino poyerekeza ndi machubu owonekera, makamaka pogwira ntchito ndi zitsanzo zotsika kwambiri kapena ma tempulo a DNA otsika kwambiri. Mbaliyi imalola kutsata kwachitsanzo kosavuta ndi kutsimikizira, kuchepetsa chiopsezo cha zolakwika za pipetting ndi kuipitsidwa kwa zitsanzo.

4.Wosabala ndi RNase/DNase-Free

Machubu athu a PCR amakhala osabala ndipo ndi ovomerezeka a RNase/DNase-free, kupereka chitetezo chowonjezera ku kuwonongeka kwa nucleic acid. Izi ndizofunikira makamaka pochita ndi zokonda zovutirapo kapena zotsika kwambiri, kuwonetsetsa kuti zotsatira zanu sizikusokonezedwa ndi zoipitsa.

5.Eco-Wochezeka komanso Wokhazikika

ACE yadzipereka kupanga zinthu zatsopano komanso zoteteza chilengedwe. Machubu athu a 0.1mL White 8-Strip PCR amapangidwa kuchokera kuzinthu zapamwamba kwambiri, zomwe zimatha kubwezeredwanso, kuchepetsa momwe chilengedwe chimagwirira ntchito mu labotale yanu. Sankhani ACE ndikuthandizira tsogolo labwino pakufufuza zamankhwala.

6.Zotengera Zosavuta komanso Zosavuta

Opakidwa mizere isanu ndi itatu, machubu awa amasunga malo mufiriji yanu ndi pa benchi yanu ya labu, kuwapanga kukhala abwino kwa mapulogalamu a PCR apamwamba kwambiri. Mizere imatha kupatulidwa mosavuta kukhala machubu apawokha, kukupatsirani kusinthasintha mumayendedwe anu ndikuwonetsetsa kuti ndizotsika mtengo.

Momwe Mungakulitsire PCR Yanu Pogwiritsa Ntchito Machubu a PCR a ACE

Kuti mupindule bwino ndi machubu a ACE a 0.1mL White 8-Strip PCR, lingalirani malangizo awa:

1.Muziziziritsatu machubu anu: Ikani zingwezo mu cycler yotentha musanayambe kuthamanga kuti muwonetsetse kuti kutentha kuli kofulumira komanso kofanana.

2.Gwiritsani ntchito ma reagents apamwamba: Limbikitsani machubu anu ndi mitundu yosiyanasiyana ya PCR ya ACE kuti muwonjezere magwiridwe antchito ndi zokolola.

3.Chepetsani kutuluka kwa nthunzi: Onetsetsani kuti zivundikirozo zimapanga chisindikizo cholimba kuti zisatuluke, zomwe zingakhudze zotsatira zanu za PCR.

4.Sungani bwino: Sungani machubu anu pa kutentha koyenera kosungirako kuti mukhalebe wosabala komanso wopanda RNase/DNase.

Mapeto

Kuyika ndalama pazakudya zapamwamba kwambiri za PCR ndikofunikira kuti mupeze zotsatira zodalirika komanso zopangikanso za PCR. Machubu a ACE a 0.1mL White 8-Strip PCR adapangidwa kuti akwaniritse zofunikira kwambiri pa kafukufuku wamakono wa biology ndi matenda. Ndi kuphatikiza kwawo kulondola, kusasinthika, komanso udindo wa chilengedwe, machubu awa ndi chisankho chabwino kwambiri pakukwaniritsa zoyeserera zanu za PCR. Pitanitsamba lathu lazinthukuti mudziwe zambiri ndikuyitanitsa zomwe mumapeza lero. Limbikitsani zoyeserera zanu za PCR ndi machubu apamwamba kwambiri a ACE a 0.1mL white 8-strip PCR ndikupititsa patsogolo kafukufuku wanu.