M'malo mwazomwe zimachitika pazinthu zamagetsi Kukwaniritsa zotsatira zabwino za PCR sikutanthauza kuti silingane ndi chida cholondola komanso zopumira komanso zomata kwambiri, makamaka machubu a PCR. Lero, ndine wokondwa kuyambitsaNjuga's 0.1ml pcr mabatani 8-opangidwa kuti apititse zoyeserera zanu za PCR ndikuwonetsetsa kuti ndi kulongosola bwino komanso kubereka. Tiyeni tilowe m'magulu ndikupindulitsa omwe amapangitsa kuti machubu awa achuma chofufumitsa kapena labu matenda.
Chifukwa Chiyani Tiyenera Kusankha Mababu Oyera 0.1ml PCR PCR?
1.Mtundu wosasinthika ndi wosasinthika
Tikatero, timadzikuza tokha kuti tizitsogolera anthu ambiri azachipatala. Tizilombo tating'onoting'ono tomwe timapangidwa 8.1ml timer 8-strip time opangidwa pogwiritsa ntchito ukadaulo woumba zam'madzi ndi njira zolimbikitsira. Izi zikuwonetsetsa kuti chubu chilichonse chimakumana ndi miyezo yapamwamba kwambiri yofananira, yofanana, komanso yosakhazikika, yofunikira pcr yoyeserera pa kuyesa kwanu konse.
2.Okhazikika pa pcr protocols
Mapangidwe a mabatani athu 8 a mabatani omwe amakhala oyenera kukhala osasunthika mumizereni ozungulira, kuchepetsa mipata yomwe imatha kubweretsa kutentha komanso kuzizira. Kukula kwa 0.1ml ndikoyenera kugwiritsa ntchito ma pcr osiyanasiyana, kuchokera ku DRNA Kukulitsidwa kwa DNA, kuwonetsa zovuta zambiri zamagetsi, onetsetsani kuti mutha kukhala ndi chiwongola dzanja nthawi zonse.
3.Utoto woyera kuti ukhale wowoneka bwino
Mtundu Woyera wa machubu awa amapereka bwino poyerekeza ndi machubu owoneka bwino, makamaka pogwira ntchito ndi zitsanzo zotsika kapena ma template otsika kwambiri. Izi zimathandizira kutsata zitsanzo zosavuta komanso kutsimikizira, kuchepetsa chiopsezo cha zolakwa ndi ziwonetsero.
4.Wosabala ndi rnase / dnase-free
Mapulogalamu athu a PCR amabwera osabala ndipo ali ndi ma rnase / dnase-free, osamasuka, kupereka chitetezo chowonjezera motsutsana ndi manyuchi a acid a acid kuwonongeka. Izi ndizofunikira makamaka mukamachita ndi zigoli zowoneka bwino kapena zochepa, onetsetsani kuti zotsatira zanu sizinyalanyazidwa ndi zodetsa nkhawa.
5.Eco-ochezeka komanso mokhazikika
ACE imadzipereka kupanga zinthu zatsopano komanso zochezeka zachilengedwe. Tizilombo tating'onoting'ono tofiirira 8 -1ml timapangidwa kuchokera ku zinthu zapamwamba kwambiri, zopangidwanso, ndikuchepetsa mawonekedwe a chilengedwe cha ntchito zanu zapantchito. Sankhani ACE ndikuthandizirani mtsogolo mwazofufuza za kafukufuku.
6.Mtengo wokwera mtengo komanso wosavuta
Kutalikirana pamiyala isanu ndi itatu, machubu awa amasunga malo mufiriji yanu komanso pa benchi yanu ya lab, ndikuwapangitsa kukhala abwino pakugwiritsa ntchito PCR-PCR Kugwiritsa ntchito. Mizere imatha kupatukana mosavuta mu machubu payekha, kusinthitsa kusintha kwanu pokonzanso mtengo.
Momwe mungakwaniritsire PCR yanu pogwiritsa ntchito machubu a PCR
Kuti mudziwe bwino zabwino za 0.1ml pcr 8-strip pcr 8-strip pcr, lingalirani malangizowa:
1.Pre-ozizira machubu anu: Ikani mabatani mu wotchinga wozungulira musanayambe kuthamanga kuti awonetsetse mwachangu komanso kutentha kwa yunifolomu mwachangu.
2.Gwiritsani ntchito ma reagent apamwamba kwambiri: Kulima machubu anu ndi mitundu ya PCR ya PCR kuti apititse magwiridwe antchito ndi zokolola.
3.Muchepetse Kusintha: Onetsetsani kuti lids imapanga chidindo cholimba kuti mupewe kusinthasintha, komwe kumatha kukhudza zotsatira zanu za PCR.
4.Sungani bwino: Sungani machubu anu osungirako kutentha kuti musunge zinthu zosayenera komanso zaukhondo / dnase.
Mapeto
Kuyika ndalama zapamwamba kwambiri pa PCR ndizofunikira kuti mukwaniritse zotsatira zodalirika komanso zotsatira za PCR. Ace's 0.1ml PCR Mababu ofukula 8-strip amapangidwa kuti akwaniritse zofunika kwambiri pakufufuza kwamakono kwa kafukufuku wa biology biology ndi matenda. Ndi kuphatikiza kwawo kolondola, kusasinthika, komanso udindo wawo, machubu awa ndi chisankho chabwino kwambiri pakutha kuyesa kwanu kwa PCR. Chezeratsamba lathu logulitsaKuti mudziwe zambiri ndikuwongolera zomwe mwapeza lero. Sinthani zoyeserera zanu za PCR yokhala ndi mabatani oyera a 0.1ml Oyera a PCr 8.1ml
Post Nthawi: Jan-15-2025