96-chitsime chakuya chakuya (Deep Well Plate) ndi mtundu wa mbale zokhala ndi zitsime zambiri zomwe zimagwiritsidwa ntchito m'ma laboratories. Ili ndi mapangidwe a dzenje lakuya ndipo nthawi zambiri amagwiritsidwa ntchito poyesera zomwe zimafuna zitsanzo zokulirapo kapena ma reagents. Zotsatirazi ndi zina mwamagwiritsidwe ntchito ndi njira zogwiritsira ntchito mbale 96-zitsime zakuya:
Ntchito zosiyanasiyana:
Kuwunika kwapamwamba: Pazoyeserera monga kuyesa mankhwala osokoneza bongo komanso kuwunikira laibulale yapawiri, mbale zakuya zakuya za 96 zimatha kutenga zitsanzo zambiri ndikuwongolera luso loyesera.
Chikhalidwe cha ma cell: Oyenera kuyesa zama cell omwe amafunikira kuchuluka kwa chikhalidwe, makamaka chikhalidwe cha ma cell omwe amatsatira.
Enzyme-linked immunosorbent assay (ELISA): Amagwiritsidwa ntchito muzoyeserera za ELISA zomwe zimafuna kuchuluka kwakukulu kwa machitidwe.
Kuyesera kwa mamolekyulu a biology: Monga machitidwe a PCR, DNA / RNA m'zigawo, electrophoresis chitsanzo kukonzekera, etc.
Maonekedwe a puloteni ndi kuyeretsa: Amagwiritsidwa ntchito poyesera ndi mawonekedwe a protein yayikulu kapena yofunikira kuchuluka kwa buffer.
Kusungirako kwachitsanzo kwa nthawi yayitali: Chifukwa cha kuya kwakukulu kwa dzenje, kusintha kwa voliyumu kwa chitsanzo panthawi yachisanu kumatha kuchepetsedwa, komwe kuli koyenera kusungidwa kwa nthawi yaitali.
Njira yogwiritsira ntchito:
Kukonzekera kwachitsanzo: Malingana ndi zosowa za kuyesera, yezani molondola kuchuluka kwa zitsanzo kapena reagent ndikuwonjezera pa chitsime cha mbale yakuya.
Kusindikiza: Gwiritsani ntchito filimu yosindikizira yoyenera kapena gasket kuti musindikize chitsimecho kuti chisasunthike kapena kuipitsidwa.
Kusakaniza: Gwirani pang'onopang'ono kapena gwiritsani ntchito pipette ya multichannel kusakaniza chitsanzo kuti muwonetsetse kuti chitsanzocho chikugwirizana ndi reagent.
Kukulitsa: Ikani mbale yakuya m'bokosi la kutentha kosasintha kapena malo ena oyenera kuti muyimire molingana ndi zoyeserera.
Zomwe mukuwerenga: Gwiritsani ntchito zida monga zowerengera ma microplate ndi ma microscopes a fluorescence kuti muwerenge zotsatira zoyeserera.
Kuyeretsa ndi kupha tizilombo toyambitsa matenda: Mukayesa, gwiritsani ntchito zotsukira zoyenera kutsukira mbale yakuya ndikuyipha.
Kusungirako: Mbale yakuya iyenera kusungidwa bwino ikatsukidwa ndi kupha tizilombo toyambitsa matenda kuti isatengeke.
Mukamagwiritsa ntchito mbale zozama za 96, mfundo zotsatirazi ziyenera kudziwidwa:
Mafotokozedwe ogwirira ntchito: Tsatirani zomwe zimachitika mu aseptic kuti mupewe kuipitsidwa kwa zitsanzo.
Kulondola: Gwiritsani ntchito pipette ya multichannel kapena makina ogwiritsira ntchito madzi kuti muwongolere kulondola kwa ntchitoyo.
Kuyika chizindikiro bwino: Onetsetsani kuti chitsime chilichonse cha chitsimecho chalembedwa bwino kuti chizizindikirike ndi kujambula.
96 - bwino kwambirimbale ndi chida chofunika kwambiri zoyesera zapamwamba mu labotale. Kugwiritsa ntchito moyenera kumatha kukulitsa luso komanso kulondola kwa kuyesako.
Nthawi yotumiza: Aug-13-2024