Micropipette mwina ndi chida chogwiritsidwa ntchito kwambiri mu labotale. Amagwiritsidwa ntchito ndi asayansi m'magawo osiyanasiyana kuphatikiza maphunziro, zipatala ndi ma labotale azamalamulo komanso kukonza mankhwala ndi katemera kuti asamutsire madzi amchere, ochepa kwambiri.
Ngakhale zingakhale zokwiyitsa komanso zokhumudwitsa kuwona thovu la mpweya pansonga ya pipette yotayika ngati silinawonedwe kapena kunyalanyazidwa, zitha kukhudza kwambiri kudalirika komanso kuchulukitsa kwa zotsatira.
Nkhani yabwino ndiyakuti pali njira zina zosavuta zomwe mungachite kuti mupewe kuphulika kwa mpweya, ndikuwongolera magwiridwe antchito a labu, kukhutitsidwa ndi oyendetsa komanso kulondola komanso kulondola kwazotsatira.
Pansipa, tikuwunika zotsatira zopeza kuwira kwa mpweya mu nsonga ya pipette ndi zomwe muyenera kuchita kenako.
Zotsatira za Bubbles muPipette Tip
Ngakhale mutagwiritsa ntchito zolondola kwambiri, pamwamba pazigawo, zosamalidwa bwino, zogwiritsidwa ntchito komanso zoyendetsedwa bwino, kudalirika kwa zotsatira zanu kungakhudzidwe ndi zolakwika za labu. Pamene thovu kulowa munsongaikhoza kukhala ndi zotsatira zingapo.
● Wogwiritsa ntchito akawona kuwira kwa mpweya ayenera kuthera nthawi kuti apereke madziwo moyenerera, tulutsani nsongayo ndikuyambanso ntchitoyo.
● Mavuvu a mpweya osazindikirika angapangitse kusamutsa kwa voliyumu yotsika, motero kumasintha kusakanikirana kwamachitidwe komwe kumapangitsa kuyesa kulephera komanso zotsatira zokayikitsa kapena zosadalirika.
Zotsatirazi zitha kukhala ndi zotsatira zingapo (1).
● Kuchepa kwa Labu Logwira Ntchito - Mayesero ndi zoyesa ziyenera kubwerezedwa, kubweretsa ndalama zogwirira ntchito ndi zakuthupi, zomwe zingakhale zokulirapo.
● Zotsatira Zokayikitsa Kapena Zolakwika - Ngati zotsatira zolakwika zatulutsidwa pangakhale zotsatira zoopsa kwambiri kuphatikizapo kusazindikira bwino komanso zotsatira za odwala.
● Kuchotsa Zolemba Pamanja - Ngati anzanu akulephera kubwereza zotsatira zanu chifukwa cha kuphulika kwa mpweya kumapangitsa kuti mapepala a zotsatira zolakwika achotsedwe.
Njira Zabwino Zopewera Kuphulika kwa Mpweya
Nthawi zambiri, thovu la mpweya mu nsonga za pipette zimachitika chifukwa cha zolakwika za opareshoni. Kusaphunzitsidwa bwino kapena kutopa nthawi zambiri ndiye vuto lalikulu.
Kupaka mapaipi ndi ntchito yaluso yomwe imafunikira chidwi cha 110%, kuphunzitsidwa koyenera komanso kuchita bwino kuti mupeze zotsatira zokhazikika komanso zolondola.
Ngakhale pali zinthu zingapo zomwe mungachite kuti muchepetse zolakwika zapaipi, m'munsimu tawunikira njira zabwino zomwe zingagwiritsidwe ntchito kupewa kutulutsa mpweya mumalangizo a pipette.
Sinthani Njira Yogwiritsa Ntchito
Pipette Pang'onopang'ono
Ngati plunger imatulutsidwa mofulumira kwambiri pamene ikufuna, mavuvu a mpweya amatha kulowetsedwa kunsonga. Izi zitha kukhala zovuta makamaka pakusamutsa zakumwa zowoneka bwino. Zofananazo zitha kuchitika ngati plunger imatulutsidwa mwachangu kwambiri ikatha.
Pofuna kupewa thovu la mpweya mukamalakalaka, samalani kugwiritsa ntchito pistoni ya ma pipette pamanja mosalala komanso pafupipafupi, kugwiritsa ntchito mphamvu yosasinthika.
Gwiritsani Ntchito Kuzama Koyenera Kumizidwa
Kulephera kumiza nsonga ya pipette mozama pansi pa meniscus ya madzi osungiramo madzi kungayambitse kupuma kwa mpweya ndipo motero kupanga kuphulika.
Komabe, kumiza nsonga yakuya kwambiri kumatha kulakalaka madzi ochulukirapo chifukwa cha kuthamanga kwambiri kapena madontho amatha kuchitika kunja kwa nsonga kotero ndikofunikira kumiza.nsonga ya pipettempaka kuya koyenera.
Kuzama kovomerezeka kumasiyanasiyana pakati pa kukula kwa pipette, mtundu ndi kupanga. Pomwe malingaliro opanga ayenera kutsatiridwa apa pali chitsogozo choperekedwa ndi National Physical Laboratory.
Kuwongolera Kuzama Kwa Kumiza Tip
Kuzama kwa Pipette (µl) & Kuzama kwa Kumizidwa (mm)
- 1-100: 2-3
- 100 - 1,000: 2 - 4
- 1,000 - 5,000: 2 - 5
Pre-WetMalangizo a Pipette
Pamene mapaipi amachuluka kuposa 10µlmalangizo a pipetteNthawi zambiri amanyowetsedwa kale powadzaza kangapo ndi madzi omwe amaperekedwa ndikuwatulutsa kuti awonongeke kuti azitha kulondola.
Kulephera kuwanyowetsa kungayambitse thovu la mpweya, makamaka mukamagwiritsa ntchito zakumwa za viscous kapena hydrophobic. Kupewa thovu la mpweya onetsetsani kuti mumanyowa kale mukamawotcha voliyumu yoposa 10µl.
Gwiritsani Ntchito Njira Zobwerera M'mbuyo Ngati Zili Zoyenera
Viscous Substances: Vuto lomwe limafala kwambiri popanga zinthu za viscous monga ma protein kapena nucleic acids solutions, glycerol ndi Tween 20/40/60/80 ndi kupangika pafupipafupi kwa thovu pomwe njira yopititsira patsogolo ikugwiritsidwa ntchito.
Kupaka mapaipi pang'onopang'ono, kugwiritsa ntchito njira yosinthira mipope kumachepetsa chiopsezo cha kupangika kwa thovu posamutsa mayankho a viscous.
ELISA Technique
Reverse pipetting imalimbikitsidwanso poika mapaipi ang'onoang'ono96 bwino micro test mbalekwa njira za ELISA. Pamene thovu la mpweya limakokedwa mu pipette kapena kuponyedwa mu zitsime powonjezera ma reagents amatha kukhudza maonekedwe a kuwala ndi zotsatira zake. Reverse pipetting tikulimbikitsidwa kuchepetsa kapena kuthetsa nkhaniyi.
Gwiritsani ntchito Ergonomic Pipettes
Ma pipette akale omwe sanapangidwe ndi ergonomics m'maganizo amafunikira kulimbikira kwambiri, mumatopa ndipo njira yanu yopangira mapaipi imakhala yosasamala komanso yosauka. Zolakwika zomwe tazitchula pamwambapa monga kutulutsa mwachangu kwa plunger zitha kuchitika pafupipafupi.
Pogwiritsa ntchito njira yowonjezereka ya ergonomic mudzatha kukhalabe ndi luso labwino kwambiri ndikupewa kupangika kwa thovu la mpweya chifukwa cha luso losauka.
Tengani Nthawi Yophunzitsa Ogwira Ntchito
Kuphunzitsa nthawi zonse ndi kuwunika ogwira ntchito mu njira za pipetting kungathe kuonetsetsa kuti zolakwika za oyendetsa galimoto ndi mapangidwe a mpweya wa mpweya akuchepetsedwa.
Ganizirani Mayankho Ambiri Odzichitira
Monga tafotokozera pamwambapa, mavuvu ambiri a mpweya amayamba ndi woyendetsa. Zitha kukhala zotheka kuchepetsa zolakwika ndi chitonthozo cha ogwiritsa ntchito pogwiritsa ntchito ma pipette amagetsi kapena nsanja yosinthira madzi mongaAgilent Bravo Liquid Handling Robot.
Gwiritsani Ntchito ZabwinoMalangizo a Pipette
Ma Micropipettes nthawi zambiri amagulidwa mosamala, koma nthawi zambiri amaganizira pang'ono za mtundu wa nsonga ya pipette yotayika. Chifukwa cha chikoka chomwe nsonga imakhala nayo pazotsatira za mapaipi, muyezo wa ISO 8655 umafunikira kuwongolera kowonjezera ngati ma pipette ndi malangizo ochokera kwa opanga osiyanasiyana akugwiritsidwa ntchito.
Izi zitha kukhala chifukwa nsonga zambiri zotsika mtengo zimatha kuwoneka bwino poyambira koma mukawerenga mosamala zitha kukhala ndi zonyezimira, zowonekera, zokanda, ndi thovu la mpweya, kapena kupindika kapena kukhala ndi zonyansa.
Kugula malangizo abwino opangidwa ndi polypropylene yapamwamba kumachepetsa kupezeka kwa thovu la mpweya.
Pomaliza
Kupeza thovu la mpweya pansonga yanu ya pipette kumakhudza momwe labu imagwirira ntchito komanso kusalondola komanso kulondola kwa zotsatira. Tawona zinthu zingapo zomwe mungachite kuti mupewe mavuvu a mpweya kulowansonga ya pipette.
Komabe, ngati osauka khalidwemalangizo a pipettezikupangitsa thovu la mpweya kulowa mu nsonga yanu ya pipette, mudzakhala okondwa kudziwa kuti kukwanira kwathu konsekonsemalangizo a pipetteamapangidwa mwapamwamba kwambiri ndipo amapangidwa ndi premium-grade pure polypropylene.
Kampani ya Suzhou Ace Biomedicalkutulutsa nsonga zapamwamba za 10,20,50,100,200,300,1000 ndi 1250 µL, nsonga 96 / choyikapo. Kukhalitsa kwapadera - ma racks onse a ACE amakwaniritsa zofunikira zogwiritsidwa ntchito ndi ma pipettor ambiri. Wosabala, Wosefera, RNase-/DNase-free, ndi nonpyrogenic.
Takulandirani kuti mutifunse zambiri.
Nthawi yotumiza: Dec-29-2022