Malangizo, monga zogwiritsidwa ntchito ndi ma pipette, nthawi zambiri amatha kugawidwa kukhala: ①. Malangizo osefera, ②. Malangizo okhazikika, ③. Malangizo otsika, ④. Palibe kutentha, etc.
1. nsonga ya fyuluta ndi yogwiritsidwa ntchito kuti ipewe kuipitsidwa. Nthawi zambiri amagwiritsidwa ntchito poyesera monga molekyulu biology, cytology, ndi virology.
2. Standard nsonga ndi ambiri ankagwiritsa ntchito nsonga. Pafupifupi ntchito zonse za pipetting zingagwiritse ntchito nsonga wamba, yomwe ndi njira yotsika mtengo kwambiri.
3. Zoyesera zokhala ndi zofunikira zokhudzidwa kwambiri, kapena zitsanzo zamtengo wapatali kapena ma reagents omwe ndi osavuta kukhalapo, mukhoza kusankha nsonga yochepetsetsa kuti muwonjezere mlingo wochira. Pamwamba pa nsonga ya low-adsorption yakhala ndi chithandizo cha hydrophobic, chomwe chingachepetse kupsinjika kwamadzi otsika ndikusiya zotsalira zambiri pansonga. (Chithunzicho sichinathe ndipo kukumbukira kuli kochepa)
PS: Nsonga yapakamwa yotakata ndi yabwino kuyamwa zida zowoneka bwino, DNA yamtundu, ndi madzi amtundu wama cell;
Zizindikiro zogwirira ntchito za nsonga: kutsika kwapang'onopang'ono, zosefera, zolimba, mphamvu yotsitsa ndi kutulutsa, palibe DNase ndi RNase, palibe pyrogen;
Kodi kusankha nsonga yabwino? "Bola ngati nsonga yomwe ingayikidwe ndi nsonga yomwe ingagwiritsidwe ntchito"
——Uku ndiko kumvetsetsa kwa pafupifupi onse ogwiritsa ntchito pa kusinthika kwa mutu woyamwa. Mawuwa tinganene kuti ndi oona koma osati oona.
Nsonga yomwe ingakhoze kukwera pa pipette ikhoza kupanga dongosolo la pipetting ndi pipette kuti lizindikire ntchito ya pipetting, koma kodi izi ndizodalirika? Funso likufunika apa. Kuyankha funsoli kumafuna deta kuti ilankhule.
1. Mungafune kuchita mayeso a ntchito mutafananiza pipette ndi nsonga. Mukatsuka nsongayo, chitani machitidwe angapo obwerezabwereza, yezani kuchuluka kwa zitsanzozo nthawi iliyonse, ndikulemba zomwe zawerengedwa.
2. Werengetsani kulondola ndi mwatsatanetsatane wa ntchito pipetting pambuyo akatembenuka mu voliyumu malinga ndi kachulukidwe a madzi mayeso.
3. Zomwe tiyenera kusankha ndi nsonga yolondola bwino. Ngati kulondola kwa pipette ndi nsonga sikuli bwino, zikutanthauza kuti kumangika kwa nsonga ndi pipette sikungatsimikizidwe, kotero kuti zotsatira za ntchito iliyonse sizingapangidwenso.
Ndiye pali mfundo zochepa zotani za nsonga yabwino?
Nsonga yabwino imatengera kukhazikika, kutsika, ndipo mfundo yofunika kwambiri ndi kutsatsa;
1. Tiyeni tiyankhule za taper poyamba: ngati kuli bwino, machesi ndi mfuti adzakhala abwino kwambiri, ndipo kuyamwa kwamadzimadzi kudzakhala kolondola;
2. Kukhazikika: Kukhazikika ndiko ngati bwalo pakati pa nsonga ya nsonga ndi kugwirizana pakati pa nsonga ndi pipette ndi malo omwewo. Ngati sichiri pakati chomwecho, zikutanthauza kuti concentricity si bwino;
3. Pomaliza, chofunikira kwambiri ndi kutsatsa kwathu: kutsatsa kumagwirizana ndi zinthu za nsonga. Ngati zinthu za nsonga si zabwino, zidzakhudza kulondola kwa pipetting, chifukwa mu kuchuluka kwa madzi posungira kapena chidule Kupachikidwa pa khoma, kuchititsa zolakwa pipetting;
Choncho aliyense ayenera kumvetsera mwapadera mfundo zitatu zomwe zili pamwambazi posankha mutu woyamwa
Nthawi yotumiza: Oct-30-2021