Pankhani ya kafukufuku wasayansi, makamaka m'magawo monga biochemistry, cell biology, ndi pharmacology, kusankha kwa zida za labotale kumatha kukhudza kwambiri magwiridwe antchito komanso kulondola kwa zoyeserera. Chimodzi mwazofunikira zotere ndikusankha pakati pa mbale 96-zitsime ndi 384. Mitundu yonse iwiri ya mbale ili ndi ma seti awoabwino komanso zovuta zomwe zingakhalepo. Chinsinsi cha kukhathamiritsa kwa labu ndikumvetsetsa kusiyana kumeneku ndikusankha yomwe ikugwirizana ndi zosowa zenizeni za kuyesa.

1. Voliyumu ndi Kutulutsa

Kusiyanitsa kwakukulu pakati pa mbale za 96-chitsime ndi 384 ndi chiwerengero cha zitsime, zomwe zimakhudza mwachindunji kuchuluka kwa ma reagents omwe angagwiritsidwe ntchito komanso kutulutsa kwa zoyesera. Mbale ya zitsime 96, yokhala ndi zitsime zazikulu, nthawi zambiri imakhala ndi voliyumu yochulukirapo, kupangitsa kuti ikhale yoyenera kuyeserera komwe kumafunikira ma reagents ochulukirapo kapena zitsanzo, komanso kuyesa komwe kumapangitsa kuti madzi asamavutike. Mosiyana ndi zimenezi, mbale za 384-zitsime, zokhala ndi zitsime zochulukirapo, zimalola kuti pakhale kuyesedwa kwakukulu panthawi imodzi, motero kumawonjezera kutulutsa kwambiri. Izi ndizopindulitsa makamaka pamapulogalamu apamwamba kwambiri (HTS), komwe kutha kukonza zitsanzo zambiri mwachangu ndikofunikira.

2. Kugwiritsa Ntchito Ndalama

Mtengo ndi chinthu china chofunikira kuganizira. Ngakhale mbale za 384-chitsime nthawi zambiri zimalola kuyesa kochulukira pa mbale, zomwe zingachepetse mtengo pa kuyesa, zingafunikenso zida zogwiritsira ntchito zamadzimadzi zomwe nthawi zambiri zimakhala zodula. Kuphatikiza apo, ma voliyumu ang'onoang'ono omwe amagwiritsidwa ntchito m'mbale za 384-chitsime atha kubweretsa kupulumutsa kwakukulu pamagetsi pakapita nthawi. Komabe, ma lab amayenera kulinganiza ndalama zomwe asungazi ndi ndalama zoyambira pazida zapamwamba kwambiri.

3. Sensitivity ndi Data Quality

Kukhudzika kwa zoyeserera zomwe zimachitika mu mbale 96 ndi 384-zitsime zimathanso kusiyana. Nthawi zambiri, voliyumu yayikulu m'mbale za 96-zitsime zitha kuthandizira kuchepetsa kusinthika ndikuwonjezera kubweza kwa zotsatira. Izi zimawapangitsa kukhala oyenera kuyeserera komwe kulondola ndikofunikira. Kumbali ina, mbale za 384-zitsime, zokhala ndi ma voliyumu ang'onoang'ono, zimatha kukulitsa chidwi pazoyeserera zina, monga fluorescence kapena zowunikira zowunikira, chifukwa cha kuchuluka kwa ma siginecha.

4. Kugwiritsa Ntchito Malo

Malo a labotale nthawi zambiri amakhala okwera mtengo, ndipo kusankha mbale kumatha kukhudza momwe malowa amagwiritsidwira ntchito moyenera. Ma mbale a zitsime 384 amathandizira kuyesedwa kochulukira m'malo omwewo poyerekeza ndi mbale za zitsime 96, kukulitsa bwino benchi ya labu ndi malo ofungatira. Izi zitha kukhala zopindulitsa makamaka m'ma lab omwe ali ndi malo ochepa kapena pomwe ntchito zapamwamba ndizofunikira.

5. Kugwirizana kwa Zida

Kugwirizana ndi zida za labu zomwe zilipo ndi lingaliro lina lofunikira. Ma laboratories ambiri ali kale ndi zida zomwe zimapangidwira mbale za zitsime 96, kuchokera ku maloboti opaka mapaipi kupita ku owerenga mbale. Kusintha kupita ku mbale za 384-chitsime kungafunike zida zatsopano kapena kusinthidwa kumakina omwe alipo, zomwe zitha kukhala zodula komanso zowononga nthawi. Chifukwa chake, ma labu akuyenera kuwunika mosamala ngati phindu losinthira ku mbale za 384-chitsime likuposa zovuta zomwe zingachitike.

Mapeto

Pamapeto pake, chigamulo pakati pa kugwiritsa ntchito mbale za 96-chitsime kapena 384 chimadalira zofunikira za labotale ndi mtundu wa zoyeserera zomwe zikuchitika. Pazoyeserera zomwe zimafunikira ma voliyumu okulirapo komanso komwe kukhudzika ndi kubwereza ndikofunikira, mbale zachitsime 96 zitha kukhala chisankho chabwinoko. Mosiyana ndi izi, pakugwiritsa ntchito kwambiri komanso kugwiritsa ntchito ndalama moyenera, mbale za 384-chitsime zimatha kupititsa patsogolo luso la labotale. Ma laboratories ayenera kupenda zinthu zimenezi mosamala, poganizira za mikhalidwe yawo yapadera, kuti apange chisankho chodziŵa bwino ndiponso chogwira mtima kwambiri.