Sankhani njira ya PCR Plate

Ma mbale a PCR nthawi zambiri amagwiritsa ntchito mawonekedwe a 96-well ndi 384-well, kutsatiridwa ndi 24-well ndi 48-well. Mtundu wa makina a PCR omwe agwiritsidwa ntchito komanso ntchito yomwe ikuchitika iwonetsa ngati mbale ya PCR ndiyoyenera kuyesa kwanu.
Siketi
"Siketi" ya mbale ya PCR ndi mbale yozungulira mbale. Chovalacho chingapereke kukhazikika kwabwino kwa ndondomeko ya pipetting panthawi yomanga machitidwe, ndikupereka mphamvu zamakina bwino panthawi yokonza makina. Ma mbale a PCR akhoza kugawidwa kukhala opanda masiketi, masiketi a theka ndi masiketi athunthu.
Board pamwamba
Pamwamba pa bolodi amatanthauza pamwamba pake.
Mapangidwe athunthu a gulu lathyathyathya ndi oyenera pamakina ambiri a PCR ndipo ndiosavuta kusindikiza ndikugwira.
Mapangidwe a mbale yokwezera m'mphepete ali ndi kusinthika kwabwino kwa zida zina za PCR, zomwe zimathandiza kulinganiza kupanikizika kwa chivundikiro cha kutentha popanda kufunikira kwa ma adapter, kuonetsetsa kuti kutentha kwabwino kwambiri ndi zotsatira zodalirika zoyesera.
Mtundu
Zithunzi za PCRnthawi zambiri amapezeka mumitundu yosiyanasiyana yamitundu kuti athandizire kusiyanitsa kowoneka ndi kuzindikira zitsanzo, makamaka pakuyesa kopitilira muyeso. Ngakhale mtundu wa pulasitiki ulibe mphamvu pakukula kwa DNA, pokhazikitsa zochitika zenizeni za PCR, timalimbikitsa kugwiritsa ntchito zopangira pulasitiki zoyera kapena pulasitiki yachisanu kuti tipeze fluorescence yodziwika bwino poyerekeza ndi zinthu zowonekera. Zogwiritsidwa ntchito zoyera zimathandizira kukhudzika komanso kusasinthika kwa data ya qPCR poletsa fulorosenti kuti isatuluke mu chubu. Kukanika kukachepetsedwa, siginecha yochulukirapo imawonekeranso ku chowunikira, potero kumakulitsa chiŵerengero cha ma signal-to-phokoso. Kuonjezera apo, khoma la chubu loyera limalepheretsa chizindikiro cha fulorosenti kuti chisalowetsedwe ku gawo la chida cha PCR, kupewa kutengeka kapena kuwonetsa mosagwirizana ndi chizindikiro cha fulorosenti, motero kuchepetsa kusiyana kwa kuyesa mobwerezabwereza.
Mitundu yosiyanasiyana ya zida, chifukwa cha mapangidwe osiyanasiyana a malo a chowunikira cha fluorescence, chonde onani manuf.