Makina ogwiritsira ntchito madzi amadzimadzi ali ndi zabwino zambiri akamagwira zamadzimadzi zovuta monga zamadzimadzi zowoneka bwino kapena zosasunthika, komanso ma voliyumu ochepa kwambiri. Machitidwewa ali ndi njira zoperekera zotsatira zolondola komanso zodalirika ndi zidule zina zomwe zingatheke mu mapulogalamu.
Poyamba, makina ogwiritsira ntchito madzi amadzimadzi amatha kuwoneka ovuta komanso olemetsa. Koma mukangoyamba kugwira ntchito ndi zidazi, mudzazindikira momwe zimachepetsera mayendedwe anu. Mainjiniya apanga zinthu zambiri zosiyanasiyana kuti zithandizire kugwiritsa ntchito zovuta.
Mukamagwira ma voliyumu ang'onoang'ono okhala ndi makina opangira madzi odzichitira okha, ndizotheka kulakalaka ma reagents onse ofunikira pakuchitapo chimodzi.nsonga, wolekanitsidwa ndi kusiyana kwa mpweya. Njira imeneyi imakambidwa kwambiri, makamaka pankhani ya kuipitsidwa kwa zakumwa zosiyanasiyana ndi madontho kunja kwa madzi.nsonga ya pipette. Opanga ena amalimbikitsa izi kuti asunge nthawi ndi khama. The machitidwe akhoza aspirate madzi choyamba, kenako reagent A, ndiye reagent B, etc. Aliyense madzi wosanjikiza anapatukana ndi mpweya kusiyana kupewa kusanganikirana kapena zimene kuyambira mkati nsonga. Pamene madzi amaperekedwa, onse reagents osakaniza mwachindunji ndi mabuku ang'onoang'ono ndi osambitsidwansongandi ma voliyumu akuluakulu pansonga. Nsonga iyenera kusinthidwa pambuyo pa sitepe iliyonse ya pipetting.
Njira yabwino ndiyo kugwiritsa ntchito zida zapadera zokongoletsedwa ndi ma voliyumu ang'onoang'ono, mwachitsanzo, kusamutsa ma voliyumu a 1 µL muzogawa zaulere. Izi zimawonjezera liwiro ndikupewa kuipitsidwa. Ngati ma voliyumu omwe ali pansi pa 1 µl ali ndi mipope, ndi bwino kugawira mumadzi omwe mukufuna kapena pachombocho kuti mupereke voliyumu yonseyo. Kupereka ma volume ang'onoang'ono ndi kukhudzana kwamadzimadzi kumalimbikitsidwanso pamene zakumwa zovuta monga za viscous zamadzimadzi zikutsatiridwa.
Chinthu china chothandiza kwambiri cha makina ogwiritsira ntchito madzi ndi nsonga yoviika. Pamene 1 µL yokha chitsanzo ndi aspirated munsonga, dontho lamadzimadzi nthawi zambiri limamatira kunja kwansongapa nthawi ya kusamba. N'zotheka kupanga nsonga kuti alowe mumadzimadzi mu chitsime kuti madontho ndi madontho ang'onoang'ono kunja kwa nsonga afikire zomwe zimachitika.
Kuphatikiza apo, kuyika chikhumbo ndi liwiro la kugawa komanso kutulutsa mphamvu ndi liwiro kumathandizanso. Liwiro langwiro la mtundu uliwonse wamadzimadzi ndi voliyumu zitha kukonzedwa. Ndipo kukhazikitsa magawowa kumabweretsa zotsatira zobwezeredwa kwambiri chifukwa timapanga pipette pa liwiro losiyana tsiku lililonse kutengera momwe timachitira. Kusamalira zamadzimadzi zokha kumatha kuchepetsa malingaliro anu ndikukulitsa chidaliro pamapulogalamu ovuta potenga mbali zomwe zimakukwiyitsani.
Nthawi yotumiza: Feb-07-2023