Akatswiri a labotale amatha maola ambiri tsiku lililonse atanyamula kachipangizo kakang'ono, ndipo kuwongolera bwino kwa mapaipi ndikuwonetsetsa kuti zotsatira zodalirika nthawi zambiri zimakhala zovuta. sikuti zimangotsimikizira ntchito ya kuyesa kulikonse, komanso kumawonjezera mphamvu.Kumvetsetsa zosowa za kayendedwe ka pipetting kumathandiza ogwiritsa ntchito kusankha pipettes zolondola ndi zobwerezabwereza, koma pali zinthu zina zambiri zomwe ziyenera kuganiziridwa kuti zipititse patsogolo zotsatira za pipetting ndikutsimikizira kupambana kwa zoyesera.
Mwachidule, zamadzimadzi zimagwera m'magulu atatu akuluakulu: amadzimadzi, owoneka bwino, komanso osasunthika.Zamadzimadzi zambiri zimakhala ndi madzi, zomwe zimapangitsa kuti ma pipette asamuke mpweya akhale chisankho choyamba kwa ambiri. kugwira ntchito ndi zakumwa zotsekemera kwambiri kapena zowonongeka.Kusiyana pakati pa mitundu ya pipette kukuwonetsedwa mu Chithunzi 1. Ndikofunikiranso kugwiritsa ntchito njira yolondola ya pipetting - mosasamala kanthu za mtundu wamadzimadzi - chifukwa cha zotsatira zabwino kwambiri.
Magawo awiri ovuta kwambiri omwe amakhudza zotsatira za pipetting ndi zolondola komanso zolondola (Chithunzi 2) .Kuti mukwaniritse kulondola kwakukulu kwa pipetting, kulondola, ndi kudalirika, zifukwa zingapo ziyenera kukumbukiridwa. Izi ndi zofunika chifukwa kulondola kumachepa pamene voliyumu yokhazikitsidwa ikuyandikira kuchuluka kwa pipette. zopezedwa ndi ma pipette 300 µl, pamene ma pipette 50 µl amapereka zotsatira zabwino kwambiri. Kuwonjezera apo, voliyumu yoikidwa pa mapaipi amtundu wamba akhoza kusintha panthawi ya pipetti chifukwa cha kuzungulira mwangozi kwa plunger. kusintha mosadziwa pamene pipetting kupititsa patsogolo kulondola.Calibration ndi mbali ina yofunika yomwe imathandiza kutsimikizira zotsatira zodalirika posonyeza kulondola ndi kulondola kwa pipette.Mchitidwewu ukhale wosavuta kwa wosuta; mwachitsanzo, ma pipette ena amagetsi amatha kukhazikitsa zikumbutso za calibration, kapena kusunga mbiri yakale.Sizingoganizira chabe. Nthawi zambiri zimachitika chifukwa chogwiritsa ntchito malangizo amtundu wa pipette, omwe nthawi zambiri amafuna "kugogoda." Njirayi imatambasula m'mphepete mwa nsonga ya pipette ndipo ikhoza kuchititsa kuti nsongayo idonthe kapena kuti ikhale yolakwika, kapenanso kuchititsa kuti nsongayo igwe. .Kusankha ma micropipette apamwamba kwambiri opangidwa ndi malangizo enieni amatsimikizira kugwirizana kotetezeka, kupereka mlingo wapamwamba wa kudalirika ndi zotsatira zabwino. mapaipi awo.
M'malo okwera kwambiri, ndikofunika kuti mukhale ogwira mtima momwe mungathere pamene mukukhalabe odalirika komanso osasunthika kwa ndondomeko ya pipetting.Pali njira zambiri zowonjezeretsera kuwongolera bwino kwa pipetting, kuphatikizapo kugwiritsa ntchito ma multichannel ndi / kapena pipettes zamagetsi.Zida zogwiritsidwa ntchito mosiyanasiyana nthawi zambiri amapereka mitundu ingapo yamapaipi-monga kubweza kwa mapaipi, kugawa kosinthika, kuwongolera kwadongosolo, ndi zina zambiri-kuti achepetse njirayo.Mwachitsanzo, njira monga kugawa mobwerezabwereza ndizoyenera kutulutsa ma aliquots angapo a voliyumu yomweyo popanda kudzazanso nsonga. Kugwiritsa ntchito mapaipi amtundu umodzi kusamutsa zitsanzo pakati pa mitundu yosiyanasiyana ya labware kumatha kukhala kotopetsa kwambiri komanso kosalekeza.Mapaipi a Multichannel amalola kusamutsa zitsanzo zingapo nthawi imodzi ndikuphethira kwa diso. zolakwa ndi kubwereza kupsyinjika kuvulala (RSI) .Ena pipettes ngakhale amatha kusinthasintha nsonga katayanitsidwe pa pipetting, kulola kusamutsidwa kufanana angapo zitsanzo pakati pa kukula labware osiyana ndi akamagwiritsa, kupulumutsa maola nthawi (Chithunzi 3).
Akatswiri a labotale nthawi zambiri amathera maola ambiri tsiku ndi tsiku pipetting.Izi zingayambitse chisokonezo ndipo, nthawi zambiri, ngakhale kuvulala kwa dzanja kapena mkono.Malangizo abwino kwambiri opewera zoopsazi ndi kuchepetsa nthawi yomwe mumagwira pipette mpaka nthawi yaifupi kwambiri. .Kuphatikiza pa izi, ogwiritsa ntchito ayenera kusankha micropipette yopepuka komanso yolinganizidwa bwino yokhala ndi misa pakati kuti ikhale yokhazikika.Pipette iyenera kukwanira bwino m'manja mwa ogwiritsa ntchito kumanzere ndi kumanja, kukhala ndi kapangidwe kabwino kakugwira, ndikusintha. voliyumu momasuka komanso mwachangu momwe mungathere kuti mupewe kusuntha kosafunikira.Komanso, malangizowo ndi ofunikira, monga kutsitsa nsonga ndi ejection nthawi zambiri kumafuna mphamvu zambiri kuposa pipetting ndipo pali chiopsezo chovulaza, makamaka m'makonzedwe apamwamba.Nzeru za Pipette ziyenera kuwombera kukhala m'malo ndi mphamvu zochepa, perekani kulumikizana kotetezeka, komanso kukhala kosavuta kutulutsa.
Posankha micropipette yoyenera pa ntchito yanu, ndikofunika kuyang'ana mbali iliyonse ya kayendedwe kanu.Poganizira za pipette, makhalidwe ake, mtundu ndi kuchuluka kwa madzi omwe akutsatiridwa, ndi malangizo ogwiritsidwa ntchito, asayansi akhoza kutsimikizira zolondola, zolondola komanso zodalirika. zotsatira pamene kusunga zokolola ndi kuchepetsa chiopsezo kuvulazidwa.
M'kopeli, kubwezeretsedwa kwa ma analytes oyambira kumawunikidwa ndi HPLC-MS pogwiritsa ntchito makina osakanikirana amphamvu a SPE microplates. Ubwino wa SEC-MALLS pakugwiritsa ntchito biopharmaceutical…
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Nthawi yotumiza: Jun-10-2022