CryovialsA na-ejikarị maka nchekwa cryogenic nke ahịrị cell na ihe ndị ọzọ dị egwu dị ndụ, na dewars jupụtara na nitrogen mmiri mmiri.

Enwere usoro dị iche iche metụtara na nchekwa nke mkpụrụ ndụ na nitrogen mmiri mmiri. Ọ bụ ezie na ụkpụrụ bụ isi bụ ifriizi ngwa ngwa, usoro a na-arụ ọrụ na-adabere n'ụdị cell na cryoprotectant eji. Enwere ọtụtụ ihe nchekwa na omume kacha mma ị ga-eburu n'uche mgbe ị na-echekwa mkpụrụ ndụ n'ụdị okpomọkụ dị ala.

Ihe nbipute a bu n’obi ịnye nkọwapụta etu esi echekwa cryovial na nitrogen mmiri mmiri.

Kedu ihe bụ Cryovials

Cryovial bụ obere vials nwere mkpuchi nke emebere maka ịchekwa ihe nlele mmiri na oke okpomọkụ dị oke ala. Ha na-ahụ na mkpụrụ ndụ echekwara na cryoprotectant anaghị abanye na njikọ mmiri mmiri nitrogen, na-ebelata ihe ize ndụ nke mgbaji cellular ebe ọ ka na-erite uru na mmetụta dị jụụ nke nitrogen mmiri mmiri.

A na-enwekarị vial n'ụdị dị iche iche na nhazi - ha nwere ike ịdị n'ime ma ọ bụ n'èzí na eriri ala ma ọ bụ gburugburu. Ụdị adịghị mma na nke anaghị adị ọcha dịkwa.

Onye na-ejiCyrovialsiji chekwaa mkpụrụ ndụ na Nitrogen Liquid

Ọtụtụ ụlọ nyocha NHS na ụlọ nyocha nkeonwe, yana ụlọ ọrụ nyocha ndị ọkachamara na ụlọ akụ ọbara ụdọ, usoro ihe ọmụmụ epithelial cell, immunology na stem cell biology na-eji cryovials na-echekwa sel.

Selụ echekwara n'ụzọ dị otú a gụnyere cell B na T, CHO Cells, Hematopoietic Stem na Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem na Progenitor Cells, Monocytes, Myeloma, NK Cells na Pluripotent Stem Cells.

Nchịkọta nke Otu esi echekwa Cryovials na Nitrogen Liquid

Cryopreservation bụ usoro na-echekwa mkpụrụ ndụ na ihe ndị ọzọ na-emepụta ihe ndị dị ndụ site n'ime ka ha dị jụụ na okpomọkụ dị ala. Enwere ike ịchekwa mkpụrụ ndụ n'ime nitrogen mmiri mmiri ruo ọtụtụ afọ na-enweghị nkwụsị nke ndụ cell. Nke a bụ ndepụta nke usoro eji arụ ọrụ.

Nkwadebe cell

Usoro maka ịkwadebe ihe nlele ga-adịgasị iche dabere n'ụdị sel, mana n'ozuzu, a na-anakọta mkpụrụ ndụ ma na-atụgharị ya iji mepụta pellet bara ụba nke cell. A na-eweghachite pellet a n'ime ihe ndị dị elu na-agwakọta ya na cryoprotectant ma ọ bụ ihe nchekwa cryopreservation.

Ọkara nchekwa Cryopreservation

A na-eji usoro a na-arụ ọrụ iji chekwaa mkpụrụ ndụ na gburugburu ebe okpomọkụ dị ala, a ga-edobe ha site na igbochi nhazi nke kristal intra na extracellular ya mere ọnwụ sel. Ọrụ ha bụ ịnye ebe nchekwa, nchekwa maka sel na anụ ahụ n'oge usoro oyi, nchekwa na mgbaze.

A na-agwakọta ihe ngwọta dị ka plasma oyi kpọnwụrụ ọhụrụ (FFP), ngwọta plasmalyte heparinised ma ọ bụ nke na-enweghị ọbara, ihe ngwọta na-enweghị ihe anụmanụ na-ejikọta ya na cryoprotectants dị ka dimethyl sulphoxide (DMSO) ma ọ bụ glycerol.

A na-edobe pellet nke ọzọ mmiri mmiri n'ime polypropylene cryovial dị kaSuzhou Ace Biomedical ụlọ ọrụ Cryogenic Nchekwa Vials.

Ọ dị mkpa ka ị ghara ijupụta cryovials n'ihi na nke a ga-eme ka ohere nke mgbawa na ịhapụ ọdịnaya nwere ike ime (1).

Ọnụego friza a na-achịkwa

N'ozuzu, a na-eji ọnụ ọgụgụ friza a na-achịkwa nwayọọ nwayọọ maka nchekwa sel nke ọma.

Mgbe etinyere ihe nlele n'ime vials cryogenic, a na-etinye ha na ice mmiri ma ọ bụ n'ime friji 4 ℃ ma malite usoro oyi n'ime nkeji 5. Dịka ntuziaka izugbe, a na-eme ka mkpụrụ ndụ dị jụụ n'ogo -1 ruo -3 kwa nkeji (2). A na-enweta nke a site na iji ngwa nju oyi nke mmemme ma ọ bụ site n'itinye vials n'ime igbe akpọchiri akpọchi etinyere na friza ọnụego a na-achịkwa -70°C ruo -90°C.

Nyefee na Nitrogen Liquid

A na-ebufe vial cryogenic oyi kpọnwụrụ na tankị mmiri mmiri maka oge a na-akaghị aka ma ọ bụrụhaala na a na-edobe okpomọkụ na-erughị -135 ℃.

Enwere ike nweta okpomọkụ ndị a dị oke ala site na imikpu n'ime mmiri mmiri ma ọ bụ akụkụ ikuku nitrogen.

Usoro mmiri mmiri ma ọ bụ vepo?

A na-amata nchekwa nitrogen n'ime mmiri mmiri ka ọ na-edobe oyi na-atụ n'otu oge, mana anaghị akwado ya maka ebumnuche ndị a:

• Mkpa maka nnukwu mpịakọta (omimi) nke mmiri mmiri nitrogen nke bụ ihe ize ndụ. Ọkụ ma ọ bụ asphyxiation n'ihi nke a bụ ezigbo ihe ize ndụ.
• Edere ikpe nke mmetọ obe site na nje na-efe efe dị ka aspergillus, hep B na nje na-agbasa site na mmiri mmiri nitrogen (2,3)
• Enwere ike ịnweta nitrogen mmiri mmiri ịbanye n'ime vials n'oge imikpu. Mgbe ewepụrụ na nchekwa ma kpoo ọkụ n'ime ụlọ, nitrogen na-agbasa ngwa ngwa. N'ihi nke a, vial ahụ nwere ike ịgbaji mgbe ewepụrụ ya na nchekwa nitrogen mmiri mmiri, na-emepụta ihe ize ndụ site na irighiri ihe na-efe efe na ikpughe na ọdịnaya (1, 4).

N'ihi nke a, ultra-obere okpomọkụ nchekwa na-adịkarị na vapor phase nitrogen. Mgbe a ga-echekwa ihe nlele n'oge mmiri mmiri, a ga-eji tube cryoflex pụrụ iche.

Ihe dị n'akụkụ uzuoku ahụ bụ na gradient okpomọkụ kwụ ọtọ nwere ike ime na-ebute mgbanwe okpomọkụ n'etiti -135 ℃ na -190 ℃. Nke a na-achọ ka nlezianya na ịdị uchu nyochaa ọkwa nitrogen mmiri mmiri na mgbanwe okpomọkụ (5).

Ọtụtụ ndị na-emepụta ihe na-atụ aro na cryovial kwesịrị ekwesị maka nchekwa ruo -135 ℃ ma ọ bụ maka iji naanị n'oge uzuoku.

Na-agbaze Selụ echekwara echekwabara

Usoro ịgbaze na-enwe nrụgide maka ọdịbendị oyi kpọnwụrụ, yana a chọrọ njikwa na usoro kwesịrị ekwesị iji hụ na ịdị mma, mgbake, na ịrụ ọrụ nke mkpụrụ ndụ. Usoro mgbaze nke ọma ga-adabere na ụdị sel ụfọdụ. Agbanyeghị, a na-ewere ịgbaze ngwa ngwa dịka ọkọlọtọ ka:

• Belata mmetụta ọ bụla na mgbake cellular
• Nyere aka belata oge ikpughe na solutes dị na mgbasa ozi na-ajụ oyi
• Wedata mmebi ọ bụla site na mweghachi ice

A na-ejikarị ịsa mmiri, bat bead, ma ọ bụ ngwa akpaghị aka pụrụ iche na-agbaze ihe nlele.

Ọtụtụ mgbe, a na-agbaze ahịrị cell 1 n'otu oge maka nkeji 1-2, site na iji nwayọọ na-atụgharị n'ime mmiri ịsa mmiri 37 ℃ ruo mgbe enwere ntakịrị ntakịrị ice na-ahapụ na vial tupu a sachaa ha na ebe a na-eto eto.

Maka ụfọdụ mkpụrụ ndụ dị ka embrayo mammalian, okpomọkụ na-adị ngwa ngwa dị mkpa maka nlanarị ha.

Selụ adịla njikere ugbu a maka omenala cell, ikewapụ sel, ma ọ bụ n'ihe gbasara sel sel hematopoietic - ọmụmụ viability na-ekwe nkwa iguzosi ike n'ezi ihe nke mkpụrụ ndụ sel ndị na-enye onyinye tupu ọgwụgwọ myeloablative.

Ọ bụ ihe na-emekarị ka a na-ewere obere aliquots nke nlele akwọchachara nke ejiri mee ọnụ ọgụgụ cell iji chọpụta mkpokọta mkpụrụ ndụ maka ịtinye na omenala. Ị nwere ike ịlele nsonaazụ nke usoro ikewapụ mkpụrụ ndụ wee chọpụta ịdị ndụ cell.

Omume kacha mma maka nchekwa nke Cryovials

Nchekwa nke ọma nke ihe nlele echekwara na cryovial na-adabere na ọtụtụ ihe dị na protocol gụnyere nchekwa kwesịrị ekwesị na idekọ ndekọ.

• Kewaa sel n'etiti ebe nchekwa- Ọ bụrụ na mpịakọta na-enye ohere, kewaa sel n'etiti vials ma chekwaa ha n'ebe dị iche iche iji belata ihe ize ndụ nke nkwụsị nlele n'ihi ọdịda akụrụngwa.
• Gbochie mmetọ obe- Họrọ maka vial cryogenic sterile ma ọ bụ autoclave tupu eji ya eme ihe
• Jiri vial nha kwesịrị ekwesị maka sel gị- vials na-abịa n'ụdị mpịakọta dị n'etiti 1 na 5mls. Zere ijuju vial iji belata ihe ize ndụ nke mgbawa.
• Họrọ vial cryogenic nwere eriri ime ma ọ bụ na mpụga- Ụfọdụ mahadum na-atụ aro vial eriri ime ime maka usoro nchekwa - ha nwekwara ike igbochi mmetọ n'oge ndochi ma ọ bụ mgbe echekwara na nitrogen mmiri mmiri.
• Gbochie ntapu- Jiri akara nke etinyere n'ime ihe a kpụrụ akpụ n'ime okpu skru ma ọ bụ mgbanaka O iji gbochie ntapu na mmetọ.
• Jiri koodu barcode 2D na akara akara- iji hụ na traceability, vial nwere nnukwu ebe a na-ede ihe na-eme ka vial ọ bụla nwee akara nke ọma. Barcode 2D nwere ike inye aka na njikwa nchekwa na idekọ ndekọ. Okpu mkpuchi agba bara uru maka njirimara dị mfe.
• Nlekọta nchekwa zuru oke- Iji hụ na mkpụrụ ndụ anaghị efu, arịa nchekwa kwesịrị ịdị na-enyocha ọnọdụ okpomọkụ na ọkwa nitrogen mmiri mgbe niile. Ekwesịrị itinye oti mkpu iji mee ka ndị ọrụ mara njehie.

Akpachara anya maka nchekwa

Liquid nitrogen aghọwo ihe a na-emekarị na nyocha nke oge a mana ọ na-ebu ihe ize ndụ nke mmerụ ahụ dị njọ ma ọ bụrụ na ejiri ya mee ihe na-ezighi ezi.

Ekwesịrị iyi akụrụngwa nchedo onwe onye kwesịrị ekwesị (PPE) iji belata ihe ize ndụ nke ntu oyi, ọkụ na ihe ọjọọ ndị ọzọ mgbe ị na-ejikwa nitrogen mmiri mmiri. Yiri

• Cryogenic gloves
• Uwe ụlọ nyocha
• Ọta ihu zuru oke na-eguzogide mmetụta nke na-ekpuchikwa olu
• Akpụkpọ ụkwụ mechiri emechi
• Ihe mkpuchi plastik na-adịghị agbasa

Ekwesịrị idowe friji nitrogen mmiri mmiri n'ebe ikuku na-ekuku nke ọma iji belata ihe ize ndụ nke asphyxiation - nitrogen gbapụrụ agbapụ na-ekupụ ma na-ewepụ oxygen ikuku. Ụlọ ahịa dị ukwuu kwesịrị inwe usoro mkpu ikuku oxygen dị ala.

Ịrụ ọrụ na ụzọ abụọ mgbe ị na-ejikwa nitrogen mmiri mmiri dị mma ma ekwesịrị ka amachibido iji ya na-abụghị oge ọrụ.

Cryovials iji kwado usoro ọrụ gị

Suzhou Ace Biomedical ụlọ ọrụ na-enye ọtụtụ nhọrọ nke ngwaahịa na-egbo mkpa gị cryopreservation maka ụdị sel dị iche iche. Pọtụfoliyo ahụ gụnyere ọtụtụ tubesa yana ọtụtụ cryovial na-adịghị ọcha.

Cryovial anyị bụ:

• Lab Screw Cap 0.5mL 1.5mL 2.0mL Cryovial Cryogenic Vials Conical Bottom Cryotube nwere Gasket

  ● 0.5ml,1.5ml,2.0ml nkọwapụta,na uwe mwụda ma ọ bụ na-enweghị uwe mwụda
  ● Nhazi conical ma ọ bụ guzoro onwe ya, sterile ma ọ bụ nke anaghị adị mma dị ma dị
  ● A na-eji polypropylene ọkwa ahụike eme tubes okpu
  ● PP Cryotube Vials nwere ike ịjụ oyi ugboro ugboro wee gbazee
  ●Mpụta okpu nke dị n'èzí nwere ike ibelata ihe puru omume n'oge ọgwụgwọ nlele.
  ● Kpọchie okpu cryogenic tubes Universal ịghasa eri maka ojiji
  ● Ọkụkụ dabara rotors ndị a na-ahụkarị
  ● Cryogenic tube o-ring tubes dabara ọkọlọtọ 1-inch na 2-inch, 48well, 81well,96well na 100well friza igbe.
  ● Autoclavable ka 121°C na friza ruo -86°C

  Akụkụ NO	Ihe onwunwe	Mpịakọta	CAPAgba	PCS/BAG	AKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKWỤKW ahụ
  ACT05-BL-N	PP	0.5ML	Nwa, Yellow, Blue, Red, Purple, White	500	10
  ACT15-BL-N	PP	1.5ml	Nwa, Yellow, Blue, Red, Purple, White	500	10
  ACT15-BL-NW	PP	1.5ml	Nwa, Yellow, Blue, Red, Purple, White	500	10
  ACT20-BL-N	PP	2.0ML	Nwa, Yellow, Blue, Red, Purple, White	500	10