efere miri emi nke ọma 96 (Mpempe mmiri miri emi) bụ ụdị efere ọtụtụ olulu mmiri a na-ejikarị na ụlọ nyocha. Ọ nwere ihe omimi oghere imewe na-ejikarị maka nnwale na-achọ ibu mpịakọta nke samples ma ọ bụ reagents. Ndị a bụ ụfọdụ n'ime usoro ngwa ngwa yana ụzọ ojiji nke efere olulu mmiri miri emi nke ọma 96:
Oke ngwa:
Nlele nyocha dị elu: N'ime nnwale dị ka nyocha ọgwụ na nyocha ụlọ akwụkwọ mejupụtara, efere olulu mmiri miri emi nke ọma 96 nwere ike ịnabata ihe nlele ọzọ wee kwalite arụmọrụ nnwale.
Omenala cell: Kwesịrị ekwesị maka nnwale omenala sel nke chọrọ nnukwu usoro omenala, ọkachasị omenala sel ndị na-akwado ya.
Enzyme-linked immunosorbent assay (ELISA): Ejiri ya na nnwale ELISA nke chọrọ nnukwu usoro mmeghachi omume.
Nnwale usoro ihe omimi nke mkpụrụ ndụ: dị ka mmeghachi omume PCR, mmịpụta DNA/RNA, nkwadebe sample electrophoresis, wdg.
Okwu protein na ịdị ọcha: A na-eji ya na nnwale nwere nnukwu okwu protein ma ọ bụ na-achọ nnukwu ihe nchekwa.
Nchekwa ihe nlele ogologo oge: N'ihi nnukwu oghere oghere, enwere ike ibelata mgbanwe olu nke ihe nlele n'oge oyi, nke kwesịrị ekwesị maka nchekwa ogologo oge.
Usoro ojiji:
Nkwadebe ihe atụ: Dị ka mkpa nke nnwale ahụ si dị, tụọ nke ọma ihe nlele ma ọ bụ reagent kwesịrị ekwesị ma tinye ya na olulu mmiri nke efere miri emi.
Akara: Jiri ihe nkiri mkpuchi ma ọ bụ gasket dabara adaba iji mechie efere olulu mmiri ahụ iji gbochie ikpochapụ ihe nlele ma ọ bụ mmetọ.
Ngwakọta: Jiri nwayọ maa jijiji ma ọ bụ jiri pipette multichannel gwakọta ihe nlele ahụ iji hụ na ihe nlele ahụ na-akpakọrịta zuru oke na reagent.
Ntinye: Tinye efere miri emi n'ime igbe okpomọkụ mgbe niile ma ọ bụ ebe ọzọ dabara adaba maka incubation dịka ihe nyocha chọrọ.
Data Ọgụgụ: Jiri ngwa dịka ndị na-agụ microplate na microscopes fluorescence gụọ nsonaazụ nnwale.
Nhicha na mkpochapụ: Mgbe nnwale ahụ gasịrị, jiri ncha kwesịrị ekwesị hichaa efere miri emi ma kpochapụ ya.
Nchekwa: Ekwesịrị ịchekwa efere miri emi nke ọma mgbe ihichachara na nje iji zere mmetọ.
Mgbe ị na-eji efere miri emi nke ọma 96, ekwesịrị ịdebe isi ihe ndị a:
Nkọwapụta ọrụ: Soro nkọwa ọrụ aseptic iji zere mmetọ nlele.
Izizi: Jiri pipette multichannel ma ọ bụ sistemu mmiri mmiri akpaaka iji kwalite izi ezi nke ọrụ ahụ.
Akara doro anya: Jide n'aka na olulu mmiri ọ bụla nke efere olulu mmiri ahụ bụ nke ọma maka njirimara na ndekọ dị mfe.
96-ọma miri-olulu mmiriefere bụ ngwá ọrụ dị mkpa maka nyocha dị elu na ụlọ nyocha. Ojiji nke ọma nwere ike imeziwanye arụmọrụ na izi ezi nke nnwale ahụ.
Oge nzipu: Ọgọst-13-2024