efere miri emi nke ọma 96 (Mpempe mmiri miri emi) bụ ụdị efere ọtụtụ olulu mmiri a na-ejikarị na ụlọ nyocha. Ọ nwere a miri oghere imewe na-ejikarị maka nnwale na-achọ ibu mpịakọta nke samples ma ọ bụ reagents. Ndị a bụ ụfọdụ n'ime usoro ngwa na ụzọ ojiji nke efere olulu mmiri miri emi nke ọma 96:
Oke ngwa:
Nlele nyocha dị elu: N'ime nnwale dị ka nyocha ọgwụ na nyocha ọba akwụkwọ, efere olulu mmiri miri emi nke ọma 96 nwere ike nabata ihe nlele ọzọ wee kwalite arụmọrụ nnwale.
Omenala cell: Kwesịrị ekwesị maka nnwale omenala sel nke chọrọ nnukwu usoro omenala, ọkachasị omenala sel ndị na-akwado ya.
Enzyme-linked immunosorbent assay (ELISA): Ejiri ya na nnwale ELISA nke chọrọ nnukwu usoro mmeghachi omume.
Nnwale bayoloji nke mkpụrụ ndụ: dị ka mmeghachi omume PCR, mmịpụta DNA/RNA, nkwadebe sample electrophoresis, wdg.
Okwu protein na ịdị ọcha: A na-eji ya na nnwale nwere nnukwu okwu protein ma ọ bụ na-achọ nnukwu ihe nchekwa.
Nchekwa ihe nlele ogologo oge: N'ihi nnukwu oghere oghere, enwere ike ibelata mgbanwe olu nke ihe nlele n'oge oyi, nke kwesịrị ekwesị maka nchekwa ogologo oge.
Usoro ojiji:
Nkwadebe ihe nlere: Dị ka mkpa nke nnwale ahụ si dị, tụọ nke ọma ihe nlele ma ọ bụ reagent kwesịrị ekwesị ma tinye ya na olulu mmiri nke efere miri emi.
Akara: Jiri ihe nkiri mkpuchi ma ọ bụ gasket dabara adaba iji mechie efere olulu mmiri ahụ iji gbochie ikpochapụ ihe nlele ma ọ bụ mmetọ.
Ngwakọta: Jiri nwayọ maa jijiji ma ọ bụ jiri pipette multichannel gwakọta ihe nlele ahụ iji hụ na ihe nlele ahụ na-akpakọrịta zuru oke na reagent.
Ntinye: Tinye efere miri emi n'ime igbe okpomọkụ mgbe niile ma ọ bụ ebe ọzọ dabara adaba maka ntinye dịka ihe nyocha chọrọ.
Data Ọgụgụ: Jiri ngwa dịka ndị na-agụ microplate na microscopes fluorescence gụọ nsonaazụ nnwale.
Nhicha na mkpochapụ: Mgbe nnwale ahụ gasịrị, jiri ncha kwesịrị ekwesị hichaa efere miri emi ma kpochapụ ya.
Nchekwa: Ekwesịrị ịchekwa efere miri emi nke ọma mgbe ihichachara na nje iji zere mmetọ.
Mgbe ị na-eji efere miri emi nke ọma 96, ekwesịrị ịdebe isi ihe ndị a:
Nkọwapụta ọrụ: Soro nkọwa ọrụ aseptic iji zere mmetọ nlele.
Izizi: Jiri pipette multichannel ma ọ bụ sistemu mmiri mmiri akpaaka iji kwalite izi ezi nke ọrụ ahụ.
Akara doro anya: Jide n'aka na olulu mmiri ọ bụla nke efere olulu mmiri ahụ bụ nke ọma maka njirimara na ndekọ dị mfe.
96-ọma miri-olulu mmiriefere bụ ngwá ọrụ dị mkpa maka nyocha dị elu na ụlọ nyocha. Ojiji nke ọma nwere ike imeziwanye arụmọrụ na izi ezi nke nnwale ahụ.
Oge nzipu: Ọgọst-13-2024