Vim li cas cov khoom siv hauv chav kuaj yuav tsum yog DNase thiab RNase Dawb?

Hauv kev ua haujlwm ntawm molecular biology, qhov tseeb thiab kev ntseeg tau yog qhov tseem ceeb tshaj plaws. Ib qho kev kis kab mob hauv cov khoom siv hauv chav sim tuaj yeem ua rau cov txiaj ntsig tsis raug, uas tuaj yeem ua rau muaj kev cuam tshuam loj rau kev tshawb fawb thiab kev kuaj mob. Ib qho chaw ntawm kev sib kis yog qhov muaj DNase thiab RNase enzymes. Cov enzymes no degrade DNA thiab RNA, feem, thiab tuaj yeem pom nyob rau hauv ntau yam kev lom neeg matrices. Txhawm rau txo qhov kev pheej hmoo ntawm kev kis kab mob thiab xyuas kom meej cov txiaj ntsig, kuaj cov khoom siv, xws licov lus qhia pipette, daim phiaj sib sib zog nqus, PCR daim hlau, thiab cov raj, yuav tsum yog DNase thiab RNase dawb.

DNase thiab RNase enzymes yog ubiquitous thiab tuaj yeem pom nyob rau hauv ntau qhov chaw lom neeg, nrog rau tib neeg lub cev, nroj tsuag, thiab kab mob. Lawv ua lub luag haujlwm tseem ceeb hauv cov txheej txheem ntawm tes xws li DNA fragmentation, kho DNA, thiab RNA degradation. Txawm li cas los xij, lawv qhov chaw nyob hauv chav kuaj yuav ua rau muaj kev puas tsuaj rau kev sim nrog DNA thiab RNA tsom xam.

Pipette cov lus qhia yog ib qho ntawm cov khoom siv hauv chav kuaj siv ntau tshaj plaws. Lawv tau siv rau kev tuav cov kua dej kom raug thiab meej, ua rau lawv tseem ceeb heev rau ntau daim ntawv thov xws li kev npaj ua qauv, DNA sequencing, thiab PCR. Yog tias cov lus qhia pipette tsis yog DNase thiab RNase dawb, kev sib kis tuaj yeem tshwm sim thaum lub sij hawm tso dej, ua rau cov qauv DNA lossis RNA degradation. Qhov no tuaj yeem ua rau cov txiaj ntsig tsis zoo lossis tsis lees paub, ua rau muaj kev ncaj ncees ntawm tag nrho cov kev sim.

Cov phaj sib sib zog nqus yog lwm qhov tseem ceeb hauv kev sim khoom siv, tshwj xeeb tshaj yog nyob rau hauv cov ntawv thov siab. Lawv yog siv rau cov qauv cia, serial dilutions, thiab cell kab lis kev cai. Yog tias cov phiaj xwm no tsis yog DNase thiab RNase dawb, txhua qhov DNA lossis RNA cov qauv khaws cia hauv lawv tuaj yeem ua paug, ua rau muaj kev puas tsuaj ntawm nucleic acids. Qhov no tuaj yeem cuam tshuam qhov tseeb ntawm cov kev siv qis qis xws li PCR, qPCR, lossis lwm tiam kev sib txuas.

Ib yam li ntawd, PCR daim hlau thiab cov raj yog cov khoom siv tseem ceeb hauv kev siv cov tshuaj tiv thaiv polymerase (PCR). PCR yog cov txheej txheem siv dav rau kev nthuav dav DNA ua ntu zus. Yog tias PCR daim hlau thiab cov raj muaj paug nrog DNase lossis RNase, cov txheej txheem nthuav dav tuaj yeem raug cuam tshuam, ua rau cov txiaj ntsig tsis raug thiab kev txhais lus tsis raug. DNase thiab RNase-free PCR consumables tiv thaiv qhov degradation ntawm lub hom phiaj DNA los yog RNA thaum lub sij hawm amplification txheej txheem, kom ntseeg tau tias txhim khu kev qha thiab reproducing tau.

Txhawm rau daws qhov teeb meem ntawm kev sib kis, cov khoom siv hauv chav kuaj yuav tsum tau tsim nrog cov txheej txheem tswj tau zoo thiab cov ntaub ntawv uas tau lees paub tias yog DNase thiab RNase dawb. Cov tuam txhab xws li Suzhou Ace Biomedical Technology Co., Ltd., tshwj xeeb hauv kev tsim cov khoom siv hauv chav sim uas ua tau raws li cov cai nruj no. Raws li cov chaw tsim khoom lag luam hauv kev lag luam, Suzhou Ace Biomedical Technology Co., Ltd. muab qhov tseem ceeb tshaj plaws thiab kev ntseeg siab.

Suzhou Ace Biomedical Technology Co., Ltd. nkag siab txog qhov tseem ceeb ntawm DNase thiab RNase paug hauv cov khoom siv hauv chav kuaj. Lawv cov lus qhia pipette, daim phiaj sib sib zog nqus, PCR daim hlau, thiab cov raj yog txhua yam tsim los siv cov ntaub ntawv zoo uas tau txais kev tswj xyuas nruj kom ntseeg tau tias lawv yog DNase thiab RNase dawb.

Lub tuam txhab ntiav cov txheej txheem kev tsim khoom siab heev thiab ua raws li cov qauv nruj nruj kom tshem tawm cov kev pheej hmoo ntawm kev sib kis, yog li lav tau qhov tseeb thiab txhim khu kev qha rau cov kws tshawb fawb thiab cov kws kho mob ib yam nkaus. Lawv nkag siab tias txhua qhov kev cuam tshuam hauv qhov zoo ntawm cov khoom siv hauv chav sim tuaj yeem ua rau muaj kev cuam tshuam loj heev, tsis yog hauv kev tshawb fawb nkaus xwb tab sis kuj tseem siv rau hauv kev siv tshuaj kho mob uas qhov kev kuaj pom tseeb yog qhov tseem ceeb.

Hauv kev xaus, kev sim khoom siv xws li cov lus qhia pipette, cov phiaj sib sib zog nqus, PCR daim hlau, thiab cov raj yuav tsum yog DNase thiab RNase pub dawb los xyuas kom meej qhov tseeb thiab kev ntseeg siab ntawm kev sim molecular biology. Kev sib kis nrog cov enzymes no tuaj yeem ua rau kev puas tsuaj ntawm DNA thiab RNA cov qauv, cuam tshuam qhov kev siv tau ntawm cov txiaj ntsig tau txais. Tuam txhab uas muag nyiamSuzhou Ace Biomedical Technology Co., Ltd. nkag siab txog qhov tseem ceeb ntawm kev tsim khoom siv uas ua tau raws li cov kev cai nruj no, ua rau cov kws tshawb fawb thiab cov kws kho mob ua lawv txoj haujlwm nrog kev ntseeg siab thiab raug.