Kev kuaj mob hauv vitro yog dab tsi?

In vitro diagnostics yog hais txog cov txheej txheem ntawm kev kuaj mob los yog mob los ntawm kev faib cov tshuaj lom neeg los ntawm sab nraud lub cev. Cov txheej txheem no tso siab rau ntau txoj hauv kev molecular biology, suav nrog PCR thiab nucleic acid extraction. Tsis tas li ntawd, kev tuav dej yog ib qho tseem ceeb ntawm kev kuaj mob hauv vitro.

PCR lossis polymerase saw cov tshuaj tiv thaiv yog cov txheej txheem siv los nthuav qhia cov khoom tshwj xeeb ntawm DNA. Los ntawm kev siv cov primers tshwj xeeb, PCR tso cai rau kev xaiv kom nthuav dav ntawm DNA ib ntus, uas tuaj yeem txheeb xyuas cov tsos mob ntawm tus kab mob lossis kab mob. PCR feem ntau yog siv los kuaj cov kab mob, kab mob, fungal thiab kab mob cab, nrog rau cov kab mob caj ces thiab mob qog noj ntshav.

Nucleic acid extraction yog cov txheej txheem siv los cais thiab ntxuav DNA lossis RNA los ntawm cov qauv lom neeg. Cov extracted nucleic acids ces muaj rau kev soj ntsuam ntxiv, suav nrog PCR. Nucleic acid extraction yog qhov tseem ceeb rau kev kuaj mob kom raug thiab kev npaj kho mob rau ntau yam kab mob thiab mob.

Kev tuav kua yog ib txheej txheem uas muaj kev hloov pauv, faib thiab sib tov ntawm cov kua me me hauv qhov chaw kuaj. Cov txheej txheem ua kua ua kua ua kua tau nrov zuj zus nyob rau xyoo tas los no vim lawv ua kom muaj kev nkag siab ntau dua thiab muaj tseeb dua hauv kev tshuaj ntsuam xws li PCR thiab nucleic acid extraction.

Nyob rau hauv vitro diagnostics cia siab rau cov txheej txheem molecular biology vim tias lawv tso cai rau kev tshawb nrhiav thiab tshuaj xyuas cov kab mob ntsig txog caj ces thiab cov cim molecular. Piv txwv li, PCR tuaj yeem siv los nthuav qhia cov kab mob tshwj xeeb uas cuam tshuam nrog mob qog noj ntshav ntawm lub mis, thaum nucleic acid extraction tuaj yeem siv los cais cov qog-derived DNA los ntawm cov ntshav kuaj.

Ntxiv nrog rau cov tswv yim no, ntau yam txuj ci thiab cov cuab yeej siv tau siv hauv kev kuaj mob vitro. Piv txwv li, cov khoom siv microfluidic tau siv ntau zuj zus hauv cov ntawv thov kev ua haujlwm siab thiab kev saib xyuas. Cov khoom siv no yog tsim los tswj thiab tswj cov kua dej me me, ua rau lawv zoo tagnrho rau PCR thiab lwm yam kev siv molecular biology.

Ib yam li ntawd, tiam tom ntej sequencing (NGS) thev naus laus zis tau ua lub luag haujlwm tseem ceeb hauv kev kuaj mob vitro. NGS ua rau muaj kev sib luag ntawm ntau lab ntawm DNA tawg, ua kom pom tau sai thiab raug ntawm cov kab mob cuam tshuam nrog kev hloov pauv caj ces. NGS muaj lub peev xwm los hloov kho kev kuaj mob thiab kho cov kab mob caj ces thiab mob qog noj ntshav.

Hauv cov ntsiab lus, kev kuaj mob hauv vitro yog ib feem tseem ceeb ntawm cov tshuaj niaj hnub no thiab vam khom rau cov txheej txheem biology molecular xws li PCR, nucleic acid extraction, thiab ua kua. Cov thev naus laus zis no, nrog rau cov thev naus laus zis xws li cov khoom siv microfluidic thiab NGS, tab tom hloov txoj hauv kev peb kuaj thiab kho kab mob. Raws li kev siv thev naus laus zis txuas ntxiv mus, kev kuaj mob hauv vitro yuav ua tau zoo dua thiab ua haujlwm tau zoo, thaum kawg txhim kho cov neeg mob tau txais txiaj ntsig thiab lub neej zoo.

At Suzhou Ace Biomedical,peb tau mob siab rau muab koj cov khoom siv lab zoo tshaj plaws rau txhua yam koj xav tau kev tshawb fawb. Peb ntau yam ntawm cov lus qhia pipette, PCR daim hlau, PCR raj, thiab sealing zaj duab xis yog meticulously tsim thiab crafted los xyuas kom meej precision thiab raug nyob rau hauv tag nrho koj cov kev sim. Peb cov lus qhia pipette tau sib xws nrog txhua hom loj ntawm pipettes thiab tuaj nyob rau hauv ntau qhov ntau thiab tsawg kom haum rau koj cov kev xav tau tshwj xeeb. Peb cov PCR daim hlau thiab cov raj yog tsim los ntawm cov ntaub ntawv zoo thiab raug tsim los tiv thaiv ntau lub voj voog thermal thaum tswj cov qauv kev ncaj ncees. Peb cov yeeb yaj kiab sealing muab lub kaw ntom ntom kom tiv thaiv evaporation thiab paug tawm ntawm cov khoom sab nraud. Peb nkag siab qhov tseem ceeb ntawm cov khoom siv hauv chav kuaj txhim khu kev qha thiab ua haujlwm tau zoo, yog li peb siv zog los muab cov khoom lag luam zoo tshaj plaws thiab cov kev pabcuam rau koj. Peb pab pawg kws tshaj lij yeej ib txwm muaj los pab koj txog cov lus nug lossis kev txhawj xeeb uas koj muaj.