PCR phaj yog dab tsi?

PCR phaj yog ib hom primer, dNTP, Taq DNA polymerase, Mg, template nucleic acid, tsis thiab lwm tus neeg nqa khoom koom nrog hauv cov tshuaj amplification hauv Polymerase Chain Reaction (PCR).

1. Siv PCR phaj

Nws yog dav siv nyob rau hauv lub teb ntawm noob caj noob ces, biochemistry, kev tiv thaiv kab mob, tshuaj, thiab lwm yam, tsis tsuas yog nyob rau hauv cov kev tshawb fawb theem pib xws li kev cais noob, cloning thiab nucleic acid ib theem zuj zus, tab sis kuj nyob rau hauv kev kuaj mob ntawm cov kab mob los yog txhua qhov chaw uas muaj DNA. thiab RNA. Nws yog ib qho khoom siv ib zaug hauv chav kuaj. Khoom.

2.96 zoo PCRCov khoom siv phaj

Nws cov khoom siv feem ntau yog polypropylene (PP) niaj hnub no, yog li nws tuaj yeem hloov kho kom zoo dua qub thiab qis kub hauv cov txheej txheem PCR, thiab tuaj yeem ua tiav qhov kub thiab txias kom tsis muaj menyuam. Txhawm rau kom ua tiav kev ua haujlwm siab dhau los ua ke nrog rab phom kab, tshuab PCR, thiab lwm yam, 96-zoo lossis 384-zoo PCR daim hlau yog siv ntau dua. Lub phaj zoo ua raws li SBS thoob ntiaj teb tus qauv, thiab txhawm rau hloov mus rau PCR cov tshuab ntawm cov tuam txhab sib txawv, nws tuaj yeem muab faib ua plaub hom qauv: tsis muaj tiab, ib nrab tiab, tsa tiab thiab daim tiab tag nrho raws li daim tiab tsim.

3. Cov xim tseem ceeb ntawm PCR phaj

Cov feem ntau yog pob tshab thiab dawb, ntawm cov ntawv dawb PCR zoo dua rau cov tshiab fluorescent quantitative PCR.