Hauv kev tshawb nrhiav caj ces thiab tshuaj, polymerase saw cov tshuaj tiv thaiv (PCR) yog cov txheej txheem siv los nthuav dav DNA qauv rau ntau yam kev sim. Cov txheej txheem no yog nyob ntawm cov khoom siv PCR uas yog qhov tseem ceeb rau kev sim ua tiav. Hauv tsab xov xwm no, peb tham txog cov khoom siv tseem ceeb rau kev sim PCR dav: PCR daim hlau, PCR raj, sealing daim nyias nyias, thiab cov lus qhia pipette.
PCR phaj:
PCR daim hlau yog ib qho ntawm cov khoom siv tseem ceeb tshaj plaws hauv txhua qhov kev sim PCR. Lawv tau tsim los rau kev caij tsheb kauj vab ceev ceev thiab muab cov cua sov hloov mus rau hauv lub bore kom yooj yim ntawm kev tuav. Cov phaj muaj nyob rau hauv ntau yam kev teeb tsa suav nrog 96-zoo, 384-zoo, thiab 1536-zoo.
PCR daim hlau yog ua los ntawm cov yas, uas ua rau lawv txhim khu kev qha thiab yooj yim los tuav. Tsis tas li ntawd, qee qhov PCR daim hlau yog coated tshwj xeeb los tiv thaiv kev khi ntawm DNA molecules thiab tiv thaiv kev sib kis. Kev siv PCR daim hlau yog qhov tseem ceeb los txo cov kev ua haujlwm hnyav ua ntej ua hauv microcentrifuges lossis PCR tshuab.
PCR tub:
PCR tubes yog cov raj me me, feem ntau yog ua los ntawm polypropylene, siv los tuav cov tshuaj tiv thaiv PCR thaum lub sijhawm ua kom nrov. Lawv tuaj nyob rau hauv ntau yam xim, tab sis feem ntau yog cov ntshiab thiab translucent. Clear PCR raj feem ntau yog siv thaum cov neeg siv xav saib cov DNA amplified vim lawv pob tshab.
Cov raj no yog tsim los tiv thaiv qhov kub thiab txias thiab kev kub ntxhov hauv PCR tshuab, ua rau lawv zoo tagnrho rau kev sim PCR. Ntxiv nrog rau kev nthuav dav, PCR hlab tuaj yeem siv rau lwm yam kev siv xws li DNA sequencing thiab purification thiab fragment tsom xam.
Sealing zaj duab xis:
Foob zaj duab xis yog ib daim nplaum yas uas txuas rau sab saum toj ntawm PCR phaj lossis raj los tiv thaiv evaporation thiab kev sib kis ntawm cov tshuaj tiv thaiv sib xyaw thaum PCR. Sealing films yog qhov tseem ceeb heev hauv PCR kev sim, vim tias cov tshuaj tiv thaiv sib xyaw ua ke los yog ib puag ncig kev sib kis hauv lub phaj tuaj yeem cuam tshuam qhov siv tau thiab ua tau zoo ntawm qhov kev sim.
Ua los ntawm polyethylene los yog polypropylene, nyob ntawm seb daim ntawv thov, cov yas zaj duab xis no yog kub resistant thiab autoclavable. Qee cov yeeb yaj kiab raug txiav ua ntej rau PCR daim hlau thiab cov raj, thaum lwm tus tuaj yeem yob thiab siv tau nrog ntau yam PCR daim hlau lossis cov raj.
Pipette tswv yim:
Pipette cov lus qhia yog cov khoom siv tseem ceeb rau kev sim PCR, vim lawv siv los hloov cov kua me me, xws li cov qauv lossis cov tshuaj reagents. Lawv feem ntau yog ua los ntawm polyethylene thiab tuaj yeem tuav cov kua ntim ntawm 0.1 µL txog 10 mL. Pipette cov lus qhia yog pov tseg thiab npaj rau kev siv nkaus xwb.
Muaj ob hom pipette tswv yim - lim thiab tsis lim. Cov lus qhia lim yog tsim los tiv thaiv cov kab mob aerosol los yog tee dej los ntawm qhov tshwm sim, thaum cov lus qhia tsis yog lim siv rau PCR thwmsim siv cov kuab tshuaj inorganic lossis cov tshuaj caustic.
Hauv cov ntsiab lus, PCR daim hlau, PCR raj, sealing daim nyias nyias, thiab cov lus qhia pipette tsuas yog qee yam ntawm cov khoom siv uas xav tau rau kev sim PCR. Los ntawm kev ua kom koj muaj tag nrho cov khoom siv tsim nyog, koj tuaj yeem ua tau zoo dua PCR kev sim ua tau zoo thiab nrog qhov tseeb koj xav tau. Yog li ntawd, nco ntsoov xyuas kom koj muaj txaus ntawm cov khoom siv no yooj yim rau txhua qhov kev sim PCR.
At Suzhou Ace Biomedical, peb tau mob siab rau muab koj cov khoom siv hauv chav kuaj zoo tshaj plaws rau txhua yam koj xav tau kev tshawb fawb. Peb qhov ntau ntawmcov lus qhia pipette, PCR daim hlau, PCR tubes, thiabsealing zaj duab xisyog tsim thiab crafted meticulously los xyuas kom meej precision thiab raug nyob rau hauv tag nrho koj thwmsim. Peb cov lus qhia pipette tau sib xws nrog txhua hom loj ntawm pipettes thiab tuaj nyob rau hauv ntau qhov ntau thiab tsawg kom haum rau koj cov kev xav tau tshwj xeeb. Peb cov PCR daim hlau thiab cov raj yog tsim los ntawm cov ntaub ntawv zoo thiab tau tsim los tiv thaiv ntau lub voj voog thermal thaum tuav cov qauv kev ncaj ncees. Peb cov yeeb yaj kiab sealing muab lub kaw ntom ntom kom tiv thaiv evaporation thiab paug tawm ntawm cov khoom sab nraud. Peb nkag siab qhov tseem ceeb ntawm cov khoom siv hauv chav kuaj kom ntseeg tau thiab ua tau zoo, yog li peb siv zog los muab cov khoom zoo tshaj plaws thiab cov kev pabcuam rau koj. Peb pab pawg kws tshaj lij yeej ib txwm muaj los pab koj txog cov lus nug lossis kev txhawj xeeb uas koj muaj.
Post lub sij hawm: May-08-2023