Cryovialsfeem ntau yog siv rau cryogenic cia ntawm cov kab ntawm tes thiab lwm yam khoom siv lom neeg tseem ceeb, hauv dewars uas muaj kua nitrogen.

Muaj ob peb theem koom nrog hauv kev khaws cia ntawm cov hlwb hauv cov kua nitrogen kom zoo. Whilst lub hauv paus ntsiab lus yog ib qho qeeb qeeb, cov txheej txheem tseeb ua haujlwm nyob ntawm hom cell thiab cryoprotectant siv. Muaj ntau qhov kev txiav txim siab txog kev nyab xeeb thiab kev coj ua zoo tshaj plaws los coj mus rau hauv tus account thaum khaws cov cell ntawm qhov kub thiab txias.

Cov ntawv tshaj tawm no yog tsim los muab cov ntsiab lus ntawm yuav ua li cas cryovials khaws cia hauv cov kua nitrogen.

Cryovials yog dab tsi

Cryovials yog me me, capped vials tsim los khaws cov qauv ua kua ntawm qhov kub tsis tshua muaj siab. Lawv xyuas kom meej tias cov hlwb khaws cia hauv cryoprotectant tsis tuaj yeem nkag mus rau hauv cov kua nitrogen ncaj qha, txo qhov kev pheej hmoo ntawm cov pob txha ntawm tes thaum tseem tau txais txiaj ntsig los ntawm huab cua txias ntawm cov kua nitrogen.

Cov vials feem ntau muaj nyob rau hauv ntau qhov ntim thiab cov qauv tsim - lawv tuaj yeem ua rau sab hauv lossis sab nraud nrog cov tiaj tiaj lossis puag ncig. Sterile thiab non-sterile hom kuj muaj.

Leej twg sivCyrovialskhaws Cells hauv Liquid Nitrogen

Ntau yam ntawm NHS thiab cov chaw soj nstuam ntiag tug, nrog rau cov chaw tshawb fawb tshwj xeeb hauv cov txhab nyiaj hauv cov hlab ntshav, cov kab mob epithelial cell biology, kev tiv thaiv kab mob thiab cov qia cell biology siv cryovials rau cryopreserve hlwb.

Cells khaws cia hauv txoj kev no suav nrog B thiab T Cells, CHO Cells, Hematopoietic Stem thiab Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem thiab Progenitor Cells, Monocytes, Myeloma, NK Cells thiab Pluripotent Stem Cells.

Txheej txheem cej luam ntawm Yuav Ua Li Cas Khaws Cryovials hauv Liquid Nitrogen

Cryopreservation yog txheej txheem uas khaws cov hlwb thiab lwm yam khoom siv lom neeg los ntawm kev ua kom txias rau qhov kub thiab txias. Cells tuaj yeem khaws cia hauv cov kua nitrogen rau ntau xyoo yam tsis muaj kev poob ntawm tes. Qhov no yog ib qho piv txwv ntawm cov txheej txheem ua haujlwm.

Kev npaj ntawm tes

Cov qauv qhia meej rau kev npaj cov qauv yuav txawv nyob ntawm seb hom cell, tab sis feem ntau, cov hlwb raug sau thiab centrifuged los tsim cov cell-nplua nuj pellet. Cov pellet no ces rov muab tso rau hauv lub supernatant tov nrog cryoprotectant los yog cryopreservation nruab nrab.

Cryopreservation Medium

Qhov nruab nrab no yog ua haujlwm los khaws cov hlwb hauv qhov chaw qis qis uas lawv yuav raug cuam tshuam los ntawm inhibiting tsim ntawm intra thiab extracellular crystals thiab li no cell tuag. Lawv lub luag haujlwm yog muab kev nyab xeeb, tiv thaiv ib puag ncig rau cov hlwb thiab cov ntaub so ntswg thaum lub sijhawm khov, khaws cia, thiab thawing txheej txheem.

Ib qho nruab nrab xws li cov ntshav khov tshiab (FFP), cov tshuaj heparinised plasmalyte los yog cov tshuaj tsis muaj ntshav, cov tshuaj tsis muaj tshuaj tiv thaiv tsiaj yog tov nrog cryoprotectants xws li dimethyl sulphoxide (DMSO) los yog glycerol.

Cov qauv rov ua kua pellet yog aliquoted rau hauv polypropylene cryovials xws liSuzhou Ace Biomedical tuam txhab Cryogenic Storage Vials.

Nws yog ib qho tseem ceeb kom tsis txhob overfill lub cryovials vim qhov no yuav ua rau muaj kev pheej hmoo ntawm kev tawg thiab tso tawm cov ntsiab lus (1).

Tswj Lub Freeze Rate

Feem ntau, tus nqi qis qis qis yog ua haujlwm rau kev ua tiav cryopreservation ntawm cov hlwb.

Tom qab kuaj tau aliquoted rau hauv cov vials cryogenic, lawv muab tso rau hauv cov dej khov ntub dej los yog hauv lub tub yees 4 ℃ thiab cov txheej txheem khov yog pib li ntawm 5 feeb. Raws li cov lus qhia dav dav, cov hlwb tau txias ntawm tus nqi ntawm -1 txog -3 ib feeb (2). Qhov no yog ua tiav siv lub programmable txias los yog muab cov vials rau hauv ib lub thawv insulated tso rau hauv -70 ° C txog -90 ° C tswj tus nqi freezer.

Hloov mus rau Liquid Nitrogen

Tom qab ntawd cov vials uas khov rau hauv lub thawv ntim tau raug xa mus rau lub tank ua kua nitrogen rau lub sijhawm tsis kawg, muab qhov kub thiab txias qis dua -135 ℃.

Cov kub tsis tshua muaj siab no tuaj yeem tau txais los ntawm kev nkag mus rau hauv cov kua lossis vapor theem nitrogen.

Kua lossis Vapor Phase?

Cia nyob rau hauv cov kua theem nitrogen yog paub kom tswj tau qhov txias txias nrog kiag li qhov sib xws, tab sis feem ntau tsis pom zoo rau cov laj thawj hauv qab no:

• Qhov xav tau rau qhov ntim loj (qhov tob) ntawm cov kua nitrogen uas yog qhov muaj peev xwm txaus ntshai. Hlawv lossis asphyxiation vim qhov no yog qhov txaus ntshai tiag tiag.
• Cov ntaub ntawv pov thawj ntawm kev sib kis los ntawm cov kab mob sib kis xws li aspergillus, hep B thiab kab mob kis los ntawm cov kua nitrogen nruab nrab (2,3)
• Lub peev xwm ua kua nitrogen rau hauv cov vials thaum immersion. Thaum tshem tawm los ntawm kev cia thiab sov rau chav tsev kub, cov nitrogen nthuav dav sai. Yog li ntawd, lub vial yuav tawg thaum tshem tawm ntawm cov kua nitrogen cia, tsim kom muaj kev phom sij los ntawm ob qho tib si ya cov khib nyiab thiab raug rau cov ntsiab lus (1, 4).

Rau cov laj thawj no, ultra-low kub cia yog feem ntau nyob rau hauv vapor theem nitrogen. Thaum kuaj yuav tsum tau muab cia rau hauv cov kua theem, tshwj xeeb cryoflex tubing yuav tsum tau siv.

Lub downside rau lub vapor theem yog qhov ntsug kub gradient tuaj yeem tshwm sim ua rau kub hloov pauv ntawm -135 ℃ thiab -190 ℃. Qhov no yuav tsum tau ceev faj thiab mob siab rau saib xyuas cov kua nitrogen ntau ntau thiab qhov kub thiab txias (5).

Ntau lub tuam txhab pom zoo tias cryovials tsim nyog rau kev khaws cia rau -135 ℃ lossis siv rau theem vapor nkaus xwb.

Thawing Koj Cryopreserved Cells

Cov txheej txheem thawing yog kev ntxhov siab rau cov kab lis kev cai khov, thiab kev tuav thiab cov txheej txheem yuav tsum tau ua kom muaj txiaj ntsig zoo, rov qab los, thiab ua haujlwm ntawm cov hlwb. Qhov tseeb thawing raws tu qauv yuav nyob ntawm seb hom cell tshwj xeeb. Txawm li cas los xij, ceev thawing yog suav tias yog tus qauv rau:

• Txo ib qho kev cuam tshuam ntawm cellular rov qab
• Pab txo lub sij hawm raug rau cov kuab tshuaj uas muaj nyob rau hauv lub freezing media
• Txo qhov kev puas tsuaj los ntawm cov dej khov recrystallisation

Cov da dej da dej, cov hlaws da dej, lossis cov cuab yeej siv tshwj xeeb uas siv tau yog feem ntau siv los ua cov qauv.

Feem ntau 1 kab ntawm tes yog thawed ntawm ib lub sij hawm rau 1-2 feeb, maj mam swirling nyob rau hauv ib tug 37 ℃ dej da dej kom txog thaum tsuas muaj ib tug me ntsis ntawm cov dej khov nyob rau hauv lub vial ua ntej lawv yuav tsum tau ntxuav nyob rau hauv ib tug prewarmed loj hlob nruab nrab.

Rau qee lub hlwb xws li mammalian embryos, qeeb sov yog qhov tseem ceeb rau lawv txoj sia nyob.

Cov hlwb tam sim no tau npaj txhij rau kab lis kev cai ntawm tes, kev cais tawm ntawm tes, lossis hauv cov kab mob haematopoietic qia hlwb - cov kev tshawb fawb muaj txiaj ntsig los lav qhov kev ncaj ncees ntawm cov neeg pub dawb qia hlwb ua ntej kho myeloablative.

Nws yog ib txwm xyaum coj me me aliquots ntawm cov qauv prewashed siv los ua ib lub xov tooj ntawm tes los txiav txim siab cell concentrations rau plating hauv kab lis kev cai. Tom qab ntawd koj tuaj yeem soj ntsuam cov txiaj ntsig ntawm cov txheej txheem kev rho tawm ntawm tes thiab txiav txim siab txog kev muaj sia nyob ntawm tes.

Cov kev coj ua zoo tshaj plaws rau kev khaws cia ntawm Cryovials

Kev ua tiav zoo ntawm kev khaws cia ntawm cov qauv khaws cia hauv cryovials nyob ntawm ntau lub ntsiab lus hauv cov txheej txheem suav nrog kev khaws cia kom raug thiab khaws cia.

• Split cells ntawm qhov chaw cia- Yog tias ntim tso cai, faib cov hlwb ntawm vials thiab khaws cia rau hauv qhov chaw sib cais kom txo qis kev pheej hmoo ntawm cov qauv poob vim cov khoom siv tsis ua haujlwm.
• Tiv thaiv kev sib kis- Xaiv rau ib leeg-siv sterile cryogenic vials lossis autoclave ua ntej siv tom ntej
• Siv cov vials tsim nyog rau koj lub hlwb- vials tuaj nyob rau hauv ntau qhov ntim ntawm 1 thiab 5mls. Tsis txhob overfilling vials kom txo qhov kev pheej hmoo ntawm kev tawg.
• Xaiv sab hauv lossis sab nraud threaded cryogenic vials- Internally threaded vials raug pom zoo los ntawm qee lub tsev kawm ntawv rau kev ntsuas kev nyab xeeb - lawv tuaj yeem tiv thaiv kev kis kab mob thaum sau lossis thaum khaws cia hauv cov kua nitrogen.
• Tiv thaiv kom tsis muaj dej- Siv ob-txheej cov ntsaws ruaj ruaj molded rau hauv cov ntsia hlau-cap lossis O-rings kom tiv thaiv kom tsis txhob muaj kab mob.
• Siv 2D barcodes thiab daim ntawv lo vials- txhawm rau txhawm rau txhawm rau taug qab, vials nrog thaj chaw sau ntawv loj ua rau txhua lub vial kom muaj cov ntawv sau txaus. 2D barcodes tuaj yeem pab tswj kev khaws cia thiab khaws cia. Cov kaus mom xim xim muaj txiaj ntsig zoo rau kev txheeb xyuas yooj yim dua.
• Kev tu kom txaus- Txhawm rau kom cov hlwb tsis ploj, cov hlab ntsha yuav tsum tau saib xyuas qhov ntsuas kub thiab cov kua nitrogen tas li. Alarms yuav tsum haum rau ceeb toom cov neeg siv ntawm qhov yuam kev.

Kev Tiv Thaiv Kev Nyab Xeeb

Cov kua nitrogen tau dhau los ua kev coj ua hauv kev tshawb fawb niaj hnub no tab sis muaj kev pheej hmoo ntawm kev raug mob hnyav yog tias siv tsis raug.

Cov cuab yeej tiv thaiv tus kheej kom raug (PPE) yuav tsum tau hnav kom txo qis kev pheej hmoo ntawm frostbite, kub hnyiab thiab lwm yam teeb meem tsis zoo thaum tuav cov kua nitrogen. Hnav

• Cryogenic hnab looj tes
• Laboratory tsho tiv no
• Impact resistant tag nrho lub ntsej muag ntaub thaiv npog uas tseem npog lub caj dab
• Kaw-toe khau
• Splashproof yas apron

Cov kua nitrogen cov tub yees yuav tsum tau muab tso rau hauv qhov chaw muaj cua zoo kom txo tau qhov kev pheej hmoo ntawm asphyxiation - dim nitrogen vaporizes thiab tshem tawm cov pa oxygen. Cov khw muag khoom ntim loj yuav tsum muaj lub tswb ntsuas pa oxygen tsawg.

Ua haujlwm ua khub thaum tuav cov kua nitrogen yog qhov zoo tshaj plaws thiab nws siv sab nraum lub sijhawm ua haujlwm yuav tsum raug txwv.

Cryovials los txhawb koj txoj haujlwm

Suzhou Ace Biomedical tuam txhab muaj ntau yam kev xaiv ntawm cov khoom uas ua tau raws li koj cov kev xav tau cryopreservation rau ntau hom hlwb. Cov ntaub ntawv pov thawj muaj xws li ntau yam ntawm cov tubes thiab ntau yam tsis muaj menyuam cryovials.

Peb cov cryovials yog:

• Lab Ntsia Hlau Cap 0.5mL 1.5mL 2.0mL Cryovial Cryogenic Vials Conical Hauv qab Cryotube nrog Gasket

  ● 0.5ml, 1.5ml, 2.0ml specification, nrog tiab los yog tsis muaj tiab
  ● Conical lossis tus kheej tsim tsim, tsis muaj menyuam lossis tsis muaj menyuam muaj ob leeg muaj
  ● Screw cap tubes yog ua los ntawm qib kev kho mob polypropylene
  ● PP Cryotube Vials tuaj yeem rov khov thiab thawed
  ●Tus tsim lub hau sab nraud tuaj yeem txo qhov muaj kab mob sib kis thaum lub sijhawm kho.
  ● Screw cap cryogenic tubes Universal ntsia hlau xov rau siv
  ● Tubes haum feem ntau cov rotors
  ● Cryogenic raj o-ring tubes haum tus qauv 1-nti thiab 2-nti, 48well, 81well, 96well thiab 100well freezer thawv
  ● Autoclavable rau 121 ° C thiab khov rau -86 ° C

  PART NO	KHOOM	VOLUME	CAPCOLOR	PCS/Hnab	BAGS/CAS
  ACT05-BL-N	PP	0.5ML	Dub, Daj, Xiav, Liab, Ntshav, Dawb	500	10
  ACT 15-BL-N	PP	1.5 ML	Dub, Daj, Xiav, Liab, Ntshav, Dawb	500	10
  ACT15-BL-NWS	PP	1.5 ML	Dub, Daj, Xiav, Liab, Ntshav, Dawb	500	10
  ACT20-BL-N	PP	2.0ML	Dub, Daj, Xiav, Liab, Ntshav, Dawb	500	10