Sterilizing AutoclavePipette Cov Lus QhiaNws yog ib qho tseem ceeb rau kev tswj xyuas kev nyab xeeb hauv chav kuaj thiab ua kom cov txiaj ntsig raug. Cov lus qhia tsis muaj menyuam tuaj yeem qhia txog kev kis kab mob microbial, ua rau muaj kev ua yuam kev thiab qeeb hauv kev sim. Autoclaving muaj txiaj ntsig zoo, tshem tawm cov kab mob xws li fungi thiab kab mob. Piv nrog rau lwm txoj hauv kev, nws muab kev ua kom tsis muaj menyuam, ua rau nws yog qhov tseem ceeb rau kev ua haujlwm hauv chav kuaj kom ntseeg tau.
Kev npaj rau Autoclaving Pipette Lub Tswv Yim
Cov ntaub ntawv xav tau rau Autoclaving
Txhawm rau kom tsis muaj menyuam cov lus qhia pipette, koj xav tau cov ntaub ntawv raug. Ib txwm siv cov lus qhia pipette ua los ntawm polypropylene lossis nws cov copolymer, vim tias cov ntaub ntawv no tuaj yeem tiv taus autoclaving dua. Tsis txhob siv cov lus qhia polyethylene, vim lawv yuav yaj hauv qhov kub thiab txias. Xyuas kom meej tias cov lus qhia tau sau tias "Autoclavable" kom paub meej tias lawv tsim nyog. Tsis tas li ntawd, koj yuav xav tau autoclave-safe racks los yog sterilization case los tuav cov lus qhia thaum lub sijhawm ua haujlwm. Cov racks no pab tswj kom muaj kev ncaj ncees ntawm cov lus qhia thiab ua kom muaj cua zoo rau kev ua kom tsis muaj menyuam.
Tshawb xyuas Pipette Cov Lus Qhia rau kev puas tsuaj lossis kev sib kis
Ua ntej autoclaving, tshuaj xyuas txhua lub taub hau rau cov kab nrib pleb, chips, lossis lwm yam kev puas tsuaj pom. Cov lus qhia puas tuaj yeem cuam tshuam tsis muaj menyuam thiab ua rau cov txiaj ntsig tsis raug. Tshawb xyuas cov khoom seem uas tsis muaj paug, xws li cov kua dej qhuav lossis cov khoom siv, uas tuaj yeem cuam tshuam rau kev ua kom tsis muaj menyuam. Muab pov tseg cov lus qhia uas qhia pom tias muaj kev puas tsuaj los yog kev sib kis kom muaj kev ncaj ncees ntawm koj qhov kev sim.
Ntxuav Siv Pipette Cov Lus Qhia Ua Ntej Autoclaving
Yog tias koj rov siv cov lus qhia pipette, ntxuav lawv kom huv si ua ntej autoclaving. Yaug cov lus qhia nrog dej distilled kom tshem tawm cov tshuaj seem. Rau cov kab mob tawv nqaij, siv cov tshuaj sterilizing kom tshem tawm tag nrho. Kev tu kom zoo tsis tsuas yog txhim kho kom tsis muaj menyuam tab sis tseem tiv thaiv cov khoom seem los ntawm kev cuam tshuam rau autoclave qhov kev ua tau zoo.
Loading Pipette Tips rau hauv Autoclave-Safe Racks
Muab cov lus qhia pipette tso rau hauv autoclave-kev nyab xeeb racks los yog kom tsis muaj menyuam. Npaj lawv nyob rau hauv ib txoj kev uas tso cai rau huab cua zoo. Tsis txhob overloading lub racks, vim qhov no tuaj yeem cuam tshuam txoj kev ua kom tsis muaj menyuam. Yog tias koj siv cov lus qhia tsis muaj menyuam, tsis txhob autoclave lawv dua, vim lawv twb tsis muaj menyuam lawm. Thaum thauj khoom, xyuas kom cov racks tau muab tso rau hauv kev ruaj ntseg los tiv thaiv tipping thaum lub voj voog autoclaving.
Kev npaj rau Autoclaving Pipette Lub Tswv Yim
Kev teeb tsa Autoclave
Ua ntej pib, xyuas kom lub autoclave huv si thiab ua haujlwm zoo. Xyuas cov dej reservoir thiab sau nws yog tias tsim nyog. Tshawb xyuas lub qhov rooj gasket rau tej yam tshwm sim ntawm kev hnav lossis kev puas tsuaj, vim qhov no tuaj yeem cuam tshuam txoj kev. Nco ntsoov ua raws li cov chaw tsim khoom cov lus qhia kom teeb tsa lub autoclave kom raug. Siv cov autoclave uas muaj kev tswj kom zoo ua kom tsis muaj menyuam ntawm koj cov lus qhia pipette thiab tiv thaiv kev sib kis.
Xaiv qhov raug Sterilization Cycle
Xaiv lub voj voog tsim nyog yog qhov tseem ceeb rau kev ua kom tsis muaj menyuam. Cov cycles uas muaj xws li:
- Lub ntiajteb txawj nqus: Relies ntawm ntuj dej ntws thiab yog qhov zoo tagnrho rau cov lus qhia pipette. Teem nws rau 252 ° F rau 20 feeb ntawm ib qho bar ntawm kev sib txheeb siab.
- Vacuum (prevac) voj voog: Siv lub tshuab nqus tsev kom tshem tawm cov huab cua ua ntej qhia chav, ua kom muaj kev nkag tau zoo dua.
- Lub voj voog ua kua: Tsim los rau cov thawv ntim dej tab sis tsis yog feem ntau siv rau cov lus qhia pipette.
Xaiv cov lus qhia pipette uas tuaj yeem tiv taus cov xwm txheej no yog qhov tseem ceeb rau kev tswj hwm lawv txoj kev ncaj ncees.
Loading Autoclave Safely
Thaum thauj cov autoclave, hnav cov khoom tiv thaiv tus kheej (PPE) xws li hnab looj tes, tsom iav kev nyab xeeb, thiab lub tsho tiv no. Teem lub racks nrog qhov chaw txaus nyob nruab nrab ntawm lawv kom tso cai rau chav ua haujlwm. Tsis txhob ntim cov autoclave kom nruj, vim qhov no tuaj yeem cuam tshuam rau kev ua kom tsis muaj menyuam. Xyuas kom lub hau ntawm cov tais diav yog qhib me ntsis kom cia chav nkag mus. Tsis txhob qhwv cov khoom hauv cov ntawv ci, vim nws nqus dej noo thiab tiv thaiv kom tsis muaj menyuam.
Khiav lub Autoclave thiab saib xyuas cov txheej txheem
Pib lub autoclave thiab saib xyuas cov txheej txheem ze. Txheeb xyuas qhov kub, siab, thiab lub sijhawm voj voog kom ntseeg tau tias lawv ua tau raws li qhov xav tau. Siv cov ntsuas tshuaj sab hauv, xws li hom 4 lossis hom 5 strips, kom paub meej tias cov tshuaj tua kab mob tau nkag mus rau hauv lub ntim. Kev saib xyuas txhua yam, xws li kev soj ntsuam ntsuas ntsuas, pab xyuas kom meej tias cov autoclave ua haujlwm raug. Sau cov txheej txheem rau traceability thiab kev ruaj ntseg zoo.
Txias thiab Unloading Autoclave
Thaum lub voj voog ua tiav, cia lub autoclave txias ua ntej qhib nws. Xyuas lub ntsuas ntsuas kom paub tseeb tias nws nyeem 0 PSI. Sawv tom qab lub qhov rooj thiab qhib nws maj mam kom tso cov seem seem kom zoo. Cia cov lus qhia pipette txias ib txwm nyob rau hauv lub autoclave kom tsis txhob muaj menyuam. Txhawm rau kom qhuav sai dua, hloov cov racks mus rau lub txee kom qhuav ntawm 55 ° C. Kev ua kom txias thiab tshem tawm kom zoo tiv thaiv kev puas tsuaj rau cov lus qhia zoo thiab khaws cia lawv cov kev ua tau zoo.
Post-Autoclaving Pipette Tip Siv thiab Cia
Tshem Tawm Sterilized Pipette Lub Tswv Yim Zoo
Kev tuav cov lus qhia kom tsis muaj menyuam kom raug yog qhov tseem ceeb rau kev tswj xyuas lawv qhov tsis muaj menyuam. Yuav tsum hnav hnab looj tes tiv thaiv kom tsis txhob kis kab mob ntawm daim tawv nqaij. Siv cov khoom siv tsuas yog sau tias "tsis muaj menyuam" txhawm rau txo qhov txaus ntshai. Ua ntej siv cov lus qhia, ntxuav lub pipette thiab nws cov tuav nrog 70% ethanol. Cov kauj ruam no ua kom ntseeg tau tias tsis muaj cov kab mob sib kis ua rau tsis muaj menyuam ntawm cov lus qhia. Thaum tshem tawm cov lus qhia los ntawm autoclave, tsis txhob nthuav tawm cov cua qhib rau lub sijhawm ntev. Hloov lawv ncaj qha mus rau hauv lub thawv huv, ntim khoom lossis lawv cov ntim khoom qub kom khaws lawv txoj kev ncaj ncees.
Tshawb xyuas cov lus qhia rau kev puas tsuaj tom qab ua kom tsis muaj menyuam
Tom qab autoclaving, tshuaj xyuas cov lus qhia pipette rau tej yam tshwm sim ntawm kev puas tsuaj. Saib rau warping, tawg, los yog discoloration, vim cov teeb meem no yuav cuam tshuam rau lawv kev ua tau zoo. Cov lus qhia puas tuaj yeem cuam tshuam qhov tseeb ntawm koj qhov kev sim lossis qhia cov kab mob. Tshem tawm cov lus qhia uas qhia pom qhov tsis xws luag. Cov kauj ruam tshuaj xyuas no ua kom ntseeg tau tias tsuas yog cov lus qhia zoo, tsis muaj menyuam siv hauv koj txoj haujlwm.
Khaws Cov Lus Qhia Pipette kom tswj kom tsis muaj menyuam
Kev cia kom zoo yog qhov tseem ceeb los khaws cov lus qhia pipette kom tsis muaj menyuam tom qab autoclaving. Khaws cov lus qhia rau hauv lawv cov ntim ntim qub lossis ib lub thawv airtight kom tiv thaiv kom tsis txhob muaj kab mob. Tsis txhob ntim cov thawv ntawv hauv cov ntawv ci, vim qhov no tuaj yeem cuam tshuam cov dej noo thiab txhawb kev loj hlob microbial. Muab lub thawv tso rau hauv qhov chaw txias, qhuav kom deb ntawm tshav ntuj ncaj qha thiab cov av noo. Tsis tu ncua ntxuav cov thawv ntim khoom kom tswj tau lawv cov txiaj ntsig. Cov kev coj ua no pab ua kom tsis muaj menyuam ntawm koj cov lus qhia pipette kom txog thaum lawv siv tom ntej.
Labeling thiab Organizing Sterilized Tips
Kev sau npe thiab teeb tsa koj cov lus qhia kom tsis muaj menyuam yaus ua kom zoo dua qub thiab txo qhov yuam kev. Siv cov ntawv ntshiab los qhia hnub ua kom tsis muaj menyuam thiab hom lus qhia khaws cia. Npaj cov lus qhia los ntawm qhov loj me lossis daim ntawv thov kom lawv yooj yim nrhiav tau thaum sim. Khaws thaj chaw cia kom huv si kom tsis txhob muaj kab mob sib kis. Lub koom haum tsim nyog tsis tsuas yog txuag lub sijhawm tab sis kuj ua kom ntseeg tau tias koj ib txwm muaj cov lus qhia tsis muaj menyuam npaj rau kev siv.
Feem ntau yuam kev thaum Autoclaving Pipette tswv yim
Overloading lub Autoclave
Overloading lub autoclave cuam tshuam cov txheej txheem sterilization. Thaum koj ntim ntau cov lus qhia pipette rau hauv lub chamber, chav tsis tuaj yeem ua haujlwm zoo. Qhov no ua rau kom tsis muaj menyuam tsis sib xws, tawm hauv qee cov lus qhia tsis muaj menyuam. Ib txwm npaj cov lus qhia hauv autoclave-kev nyab xeeb racks nrog qhov chaw txaus ntawm lawv. Tsis txhob stacking racks nruj heev. Kev sib nrug kom zoo ua kom cov chav nkag mus txog txhua qhov kawg, tswj lawv txoj kev ua kom tsis muaj menyuam thiab kev ncaj ncees.
Siv Cov Chaw Autoclave tsis raug
Kev teeb tsa tsis raug tuaj yeem ua rau cov lus qhia pipette puas lossis tsis ua kom tsis muaj menyuam. Piv txwv li, cov lus qhia pipette yuav tsum tsuas yog autoclaved ib zaug ntawm 121 ° C rau 10 feeb, ua raws li lub voj voog ziab ntawm 110 ° C rau 5 feeb. Siv qhov kub thiab txias dua los yog lub voj voog ntev dua tuaj yeem ua rau cov lus qhia nkig lossis ua rau cov lim dej flake. Cov kev pheej hmoo cuam tshuam nrog kev teeb tsa tsis raug suav nrog:
| Kev Nyab Xeeb Risk | Kev piav qhia |
|---|---|
| Kub kub | Los ntawm cov ntaub ntawv kub thiab autoclave chamber phab ntsa thiab qhov rooj |
| Chav hlawv | Los ntawm cov seem seem tso tawm tom qab lub voj voog |
| Kub kua txob scalds | Los ntawm boiling kua los yog nchuav rau hauv lub autoclave |
| Tes thiab caj npab raug mob | Thaum kaw lub qhov rooj autoclave |
| Lub cev raug mob | Yog tias muaj kev tawg vim yog qhov tsis tsim nyog los yog kev thauj khoom |
Ib txwm ua raws li cov chaw tsim khoom cov lus qhia los xaiv lub voj voog kom raug rau cov lus qhia autoclave pipette.
Hla cov kauj ruam ua ntej ntxuav
Kev hla cov kauj ruam ua ntej ntxuav ua rau muaj teeb meem kev sib kis. Cov tshuaj lom neeg lossis cov khoom siv roj ntsha ntawm cov lus qhia siv tuaj yeem cuam tshuam rau kev ua kom tsis muaj menyuam. Qhov no tuaj yeem ua rau:
- Pipette-rau-sample contamination, qhov twg pipette qhia cov pa paug rau hauv cov qauv.
- Sample-to-pipette contamination, qhov twg tus qauv paug lub cev pipette.
- Sample-rau-sample contamination, qhov twg residues hloov ntawm cov qauv.
Ua tib zoo ntxuav cov lus qhia nrog dej distilled los yog tshuaj tua kab mob ua ntej autoclaving. Cov kauj ruam no yog qhov tseem ceeb rau kev tiv thaiv kev sib kis kab mob thiab ua kom ntseeg tau cov txiaj ntsig.
Kev tuav tsis raug tom qab Sterilization
Kev tuav cov lus qhia tsis raug cai tuaj yeem tshem tawm cov txheej txheem kom tsis muaj menyuam. Ib txwm hnav hnab looj tes thaum tshem tawm cov lus qhia los ntawm autoclave. Tsis txhob kov cov lus qhia ncaj qha lossis nthuav tawm kom qhib cua rau lub sijhawm ntev. Hloov lawv tam sim ntawd mus rau cov thawv ntim khoom lossis cov khib nyiab tsim los rau kev siv thiab khaws cia. Cov kev coj ua no pab tswj kom tsis muaj menyuam ntawm koj cov lus qhia.
Khaws cov lus qhia nyob rau hauv cov xwm txheej uas tsis yog Sterile
Kev khaws cov lus qhia hauv cov xwm txheej uas tsis muaj menyuam yaus ua rau lawv kis mus rau cov kab mob. Siv cov thawv ntim airtight los yog cov thawv ntim ntim kom tiv thaiv kom tsis muaj menyuam. Tsis txhob qhwv cov lus qhia hauv cov ntawv ci, vim nws ntxiab ya raws thiab txhawb kev loj hlob microbial. Khaws cov lus qhia hauv qhov chaw txias, qhuav kom khaws lawv cov tsis muaj menyuam thiab cov tshuaj tiv thaiv ntawm pipette cov lus qhia. Kev khaws cia kom zoo ua kom muaj kev ncaj ncees ntawm koj cov lus qhia rau kev siv yav tom ntej.
Tswv yim: Ib txwm tshuaj xyuas cov lus qhia txog kev puas tsuaj lossis warping tom qab autoclaving. Cov lus qhia puas tuaj yeem cuam tshuam koj qhov kev sim thiab ua rau cov txiaj ntsig tsis raug.
Sterilizing pipette cov lus qhia yog qhov tseem ceeb rau kev tswj xyuas kev nyab xeeb hauv chav kuaj thiab ua kom cov txiaj ntsig raug. Kev ua kom tsis muaj menyuam kom raug tiv thaiv kom tsis txhob muaj kab mob, tiv thaiv kev ncaj ncees ntawm koj qhov kev sim, thiab txhawb nqa cov txiaj ntsig zoo.
Txhawm rau kom ua tiav, ua raws cov kauj ruam no kom ua kom tsis muaj menyuam:
- Npaj los ntawm kev tshuaj xyuas thiab tu cov lus qhia pipette.
- Autoclave siv cov kev teeb tsa kom raug thiab xyuas kom muaj cua zoo.
- Tom qab ua kom tsis muaj menyuam, tuav cov lus qhia kom zoo thiab khaws cia rau hauv cov thawv kaw kom tsis txhob muaj menyuam.
Cov ntsiab lus tseem ceeb rau kev nyab xeeb hauv chav kuaj muaj xws li:
- Siv autoclaves los tshem tawm cov kab mob microbial.
- Khaws cov lus qhia hauv lawv cov ntim khoom qub lossis cov thawv airtight.
- Tshawb xyuas cov lus qhia txog kev puas tsuaj ua ntej siv thiab tsis txhob muab tso rau hauv qhov qhib cua.
Los ntawm kev ua raws li cov kev coj ua no, koj ua kom ntseeg tau tias kev khaws cia thiab kev siv cov lus qhia tsis muaj menyuam yaus, uas txo qis kev pheej hmoo kis kab mob thiab txhim kho kev sim raug.
Post lub sij hawm: Feb-19-2025