Hauv thaj tsam ntawm kev tshawb fawb txog kev tshawb fawb, tshwj xeeb tshaj yog nyob rau hauv thaj chaw xws li biochemistry, cell biology, thiab pharmacology, kev xaiv cov khoom siv hauv chav kuaj tuaj yeem cuam tshuam qhov ua tau zoo thiab qhov tseeb ntawm kev sim. Ib qho kev txiav txim siab tseem ceeb yog kev xaiv ntawm 96-zoo thiab 384-zoo daim hlau. Ob hom phaj muaj lawv tus kheej qhov zoo thiab muaj peev xwm drawbacks. Tus yuam sij rau optimizing lab efficiency nyob rau hauv kev nkag siab txog cov kev sib txawv no thiab xaiv ib tug uas zoo tshaj plaws suits cov kev xav tau ntawm kev sim.

1. Volume thiab Throughput

Ib qho ntawm qhov sib txawv ntawm 96-zoo thiab 384-zoo daim hlau yog tus naj npawb ntawm cov qhov dej, uas cuam tshuam ncaj qha rau cov ntim ntawm cov tshuaj reagents uas tuaj yeem siv thiab dhau los ntawm kev sim. Lub phaj 96-zoo, nrog cov qhov dej loj dua, feem ntau tuav ntau qhov ntim, ua rau nws tsim nyog rau kev soj ntsuam uas xav tau ntau dua reagents lossis cov qauv, thiab rau kev sim qhov twg evaporation yuav muaj kev txhawj xeeb. Conversely, 384-Zoo daim hlau, nrog lawv cov ntom ntom ntawm cov dej zoo, tso cai rau ntau dua tus lej ntawm cov kev ua kom zoo, yog li nce kev cuam tshuam ntau. Qhov no yog qhov tshwj xeeb tshaj yog nyob rau hauv high-throughput screening (HTS) daim ntawv thov, qhov twg muaj peev xwm ua cov lej loj ntawm cov qauv sai yog qhov tseem ceeb.

2. Tus nqi siv tau

Tus nqi yog lwm qhov tseem ceeb uas yuav tau xav txog. Thaum 384-zoo daim hlau feem ntau tso cai rau ntau qhov kev ntsuam xyuas ib phaj, uas tuaj yeem txo tus nqi ib qho kev ntsuam xyuas, lawv kuj yuav xav tau cov khoom siv ua kua ntau dua thiab kim heev. Tsis tas li ntawd, cov reagent me me siv nyob rau hauv 384-zoo daim hlau tuaj yeem ua rau kom txuag tau tus nqi tseem ceeb ntawm cov tshuaj reagents dhau sijhawm. Txawm li cas los xij, cov chaw sim yuav tsum sib npaug cov nyiaj khaws cia no nrog kev nqis peev thawj zaug hauv cov cuab yeej siv qib siab dua.

3. Kev nkag siab zoo thiab cov ntaub ntawv zoo

Lub rhiab heev ntawm kev soj ntsuam ua nyob rau hauv 96-zoo piv rau 384-zoo daim hlau kuj tseem txawv. Feem ntau, qhov ntim loj dua hauv 96-zoo daim hlau tuaj yeem pab txo qis kev hloov pauv thiab txhim kho cov txiaj ntsig rov ua dua tshiab. Qhov no ua rau lawv haum rau kev sim qhov twg precision yog qhov tseem ceeb. Ntawm qhov tod tes, 384-zoo daim hlau, nrog cov ntim me me, tuaj yeem ua rau muaj kev nkag siab zoo hauv qee qhov kev ntsuam xyuas, xws li cov tshuaj fluorescence lossis luminescence-raws li kev soj ntsuam, vim tias qhov siab dua ntawm cov teeb liab.

4. Kev siv qhov chaw

Qhov chaw kuaj mob feem ntau yog tus nqi, thiab kev xaiv ntawm cov phaj tuaj yeem cuam tshuam qhov chaw siv tau zoo npaum li cas. 384-zoo daim hlau ua kom muaj kev ntsuam xyuas ntau dua los ua hauv tib lub cev qhov chaw piv rau 96-zoo daim hlau, ua kom muaj txiaj ntsig zoo tshaj plaws hauv lub rooj ntev zaum thiab qhov chaw incubator. Qhov no tuaj yeem tau txais txiaj ntsig tshwj xeeb hauv cov chaw sim nrog qhov chaw txwv lossis qhov chaw ua haujlwm siab tshaj plaws yog qhov tseem ceeb.

5. Cov khoom siv sib xws

Kev sib raug zoo nrog cov khoom siv hauv chav kuaj uas twb muaj lawm yog lwm qhov kev txiav txim siab tseem ceeb. Ntau lub chaw soj nstuam twb muaj cov cuab yeej tsim los rau 96-zoo daim hlau, los ntawm cov neeg hlau xa mus rau cov neeg nyeem ntawv. Kev hloov mus rau 384-zoo daim hlau yuav xav tau cov cuab yeej tshiab lossis kev hloov kho rau cov tshuab uas twb muaj lawm, uas tuaj yeem raug nqi thiab siv sijhawm. Yog li ntawd, cov chaw kuaj mob yuav tsum ua tib zoo soj ntsuam seb puas muaj txiaj ntsig ntawm kev hloov mus rau 384-zoo daim hlau ntau dua cov kev cov nyom no.

Xaus

Thaum kawg, qhov kev txiav txim siab ntawm kev siv 96-zoo lossis 384-zoo daim hlau hinges ntawm cov kev cai tshwj xeeb ntawm chav kuaj thiab qhov xwm txheej ntawm kev sim ua. Rau cov kev sim uas xav tau ntau dua thiab qhov twg rhiab heev thiab rov tsim dua yog qhov tseem ceeb, 96-zoo daim hlau yuav yog qhov kev xaiv zoo dua. Conversely, rau high-throughput daim ntaub ntawv thiab tus nqi efficiency nyob rau hauv cov nqe lus ntawm reagent siv, 384-zoo daim hlau yuav ho txhim khu kev soj ntsuam efficiency. Cov chaw kuaj mob yuav tsum ntsuas cov yam ntxwv no ua tib zoo, txiav txim siab txog lawv qhov xwm txheej tshwj xeeb, kom paub meej tshaj plaws thiab kev xaiv zoo.