Polymerase chain reaction (PCR) yog cov txheej txheem siv dav hauv molecular biology rau kev nthuav dav ntawm DNA tawg. PCR suav nrog ob peb kauj ruam, suav nrog kev nthuav tawm, kev tshem tawm, thiab txuas ntxiv. Kev vam meej ntawm cov txheej txheem no feem ntau nyob ntawm qhov zoo ntawm PCR daim hlau thiab cov raj siv. Muaj ntau ntau yam uas yuav tsum tau xav txog thaum xaiv PCR daim hlau thiab cov raj tsim nyog rau koj daim ntawv thov.Ntawm no yog qee yam tseem ceeb uas yuav tsum xav txog:
1. Muaj peev xwmPCR daim hlauthiab cov raj tuaj nyob rau hauv qhov ntau thiab tsawg thiab muaj peev xwm. Qhov kev xaiv ntawm qhov loj thiab muaj peev xwm nyob ntawm qhov ntau ntawm DNA uas yuav tsum tau ua kom muaj zog hauv ib qho tshuaj tiv thaiv. Piv txwv li, yog tias koj xav tau kom nthuav dav me me ntawm DNA, koj tuaj yeem xaiv lub raj me me. Yog hais tias DNA loj npaum li cas yuav tsum tau amplified, lub phaj nrog lub peev xwm loj tuaj yeem xaiv tau.
2. Cov khoom siv PCR daim hlau thiab cov raj tuaj yeem ua los ntawm cov khoom sib txawv xws li polypropylene, polycarbonate lossis acrylic. Polypropylene yog cov khoom siv feem ntau vim nws cov tshuaj lom neeg thiab kub ua haujlwm. Nws kuj tseem kim dua piv rau lwm cov ntaub ntawv. Polycarbonates thiab acrylics yog kim dua, tab sis muaj qhov pom tseeb zoo dua thiab zoo tagnrho rau PCR lub sijhawm.
3. Thermal conductivity PCR muaj ntau yam thermal cycles, yuav tsum tau ceev cua sov thiab txias ntawm cov tshuaj tiv thaiv sib tov. Yog li ntawd, PCR daim hlau thiab cov raj yuav tsum muaj cov thermal conductivity zoo los xyuas kom meej cov cua sov thiab txias ntawm cov tshuaj tiv thaiv sib tov. Phaj nrog cov phab ntsa nyias thiab cov chaw tiaj tus yog qhov zoo tagnrho rau kev ua kom sov siab tshaj plaws.
4. Compatibility PCR daim hlau thiab cov raj yuav tsum sib haum nrog lub thermal cycler koj siv. Daim phiaj thiab cov raj yuav tsum muaj peev xwm tiv taus qhov kub thiab txias uas yuav tsum tau ua kom nthuav dav ntawm DNA tawg. Nco ntsoov sab laj nrog cov chaw tsim khoom thermal cycler rau cov phiaj xwm pom zoo thiab cov raj.
5. PCR daim hlau thiab cov raj tuaj yeem raug kaw siv ntau txoj hauv kev xws li cov ntsaws ruaj ruaj, nplaum zaj duab xis lossis lub hau. Thaum tshav kub kub sealing yog txoj kev nyab xeeb tshaj plaws thiab muab lub zog tiv thaiv kev sib kis.
6. Sterilization PCR daim hlau thiab cov raj yuav tsum tsis pub muaj cov kab mob uas yuav cuam tshuam cov tshuaj tiv thaiv. Yog li ntawd, lawv yuav tsum tau sterilized ua ntej siv. Nws yog ib qho tseem ceeb uas yuav tau xaiv cov phaj thiab cov raj uas yooj yim kom tsis muaj menyuam thiab tiv taus cov tshuaj thiab cov cua sov kom tsis muaj menyuam.
Hauv cov ntsiab lus, xaiv qhov zoo PCR phaj thiab cov hlab yog qhov tseem ceeb rau kev ua tiav DNA amplification. Qhov kev xaiv nyob ntawm ntau yam ntawm daim ntawv thov, tus nqi ntawm DNA amplified, thiab compatibility nrog thermal cyclers.
Suzhou Ace Biomedical Technology Co., Ltd. muaj ntau yam zoo PCR daim hlau thiab cov raj hauv qhov sib txawv, muaj peev xwm thiab cov ntaub ntawv kom tau raws li qhov xav tau ntawm txhua tus neeg tshawb fawb.
Post lub sij hawm: May-17-2023