5 Feem ntau yuam kev kom zam thaum siv Pipette tswv yim hauv chav kuaj
1. Xaiv qhov yuam kevPipette Tip
Xaiv qhov raug pipette taub yog qhov tseem ceeb rau qhov tseeb thiab qhov tseeb ntawm koj qhov kev sim. Ib qho yuam kev feem ntau yog siv qhov tsis ncaj ncees lawm hom lossis qhov loj ntawm lub taub pipette. Txhua lub tswv yim yog tsim los rau cov ntawv thov tshwj xeeb, thiab siv cov lus qhia tsis raug tuaj yeem ua rau cov txiaj ntsig tsis sib xws thiab cov khoom siv pov tseg.
Txhawm rau zam qhov yuam kev no, nco ntsoov xa mus rau cov chaw tsim khoom cov lus qhia lossis sab laj tus kws tshaj lij hauv thaj chaw. Xav txog yam xws li kev sib raug zoo nrog cov pipette, cov qauv ntim xav tau, thiab hom kev sim uas koj tab tom ua. Los ntawm xaiv qhov tsim nyog pipette taub hau, koj tuaj yeem ua kom pom kev ua tau zoo thiab txhim khu kev qha.
2. Kev Taw Qhia Tsis Zoo
Kev sib txuas tsis raug ntawm lub taub pipette yog lwm qhov yuam kev uas tuaj yeem cuam tshuam qhov tseeb thiab qhov tseeb. Yog hais tias lub taub hau tsis ruaj ntseg, nws yuav loosen los yog detach thaum lub sij hawm pipetting txheej txheem, ua rau cov qauv poob thiab paug.
Txhawm rau zam qhov no, ua raws li cov chaw tsim khoom cov lus qhia rau kev txuas lub taub pipette kom raug. Xyuas kom meej tias lub taub hau haum nruj thiab ruaj ntseg rau lub pipette nozzle. Tsis tas li ntawd, tsis tu ncua tshuaj xyuas cov lus qhia rau cov cim qhia ntawm kev hnav lossis kev puas tsuaj, thiab hloov nws yog tias tsim nyog. Kev sib txuas lus zoo yog qhov tseem ceeb rau kev txhim khu kev qha thiab rov tsim dua.
3. Overpipetting lossis Underpipetting
Qhov tseeb pipetting yuav tsum ua tib zoo ntsuas thiab hloov cov ntim cov kua uas xav tau. Ob qho yuam kev uas tuaj yeem tshwm sim thaum tus txheej txheem no yog overpipetting thiab underpipetting. Overpipetting hais txog ntau tshaj qhov xav tau ntim, thaum underpipetting txhais tau tias pipetting tsawg dua li qhov xav tau.
Ob qho kev ua yuam kev tuaj yeem ua rau muaj qhov yuam kev loj hauv koj qhov kev sim. Overpipetting tuaj yeem ua rau dilution ntawm cov qauv los yog reagents, whereas underpipetting tuaj yeem ua rau cov concentrations tsis txaus lossis cov tshuaj tiv thaiv sib tov.
Txhawm rau kom tsis txhob overpipetting lossis underpipetting, nco ntsoov xyaum cov txheej txheem pipetting kom zoo. Paub koj tus kheej nrog lub pipette calibration thiab pipetts txwv. Teem lub ntim raws li, xyuas kom meej pipeting ntawm qhov xav tau ntim. Tsis tu ncua calibrate koj pipettes kom muaj tseeb thiab precision.
4. Kov lub thawv qauv
Kev kis kab mob yog ib qho kev txhawj xeeb loj hauv txhua qhov chaw kuaj mob. Ib qho yuam kev uas cov kws tshawb fawb tau ua yog yuam kev kov cov qauv ntim nrog lub hau pipette. Qhov no tuaj yeem qhia cov khoom txawv teb chaws lossis cov khoom siv rau hauv cov qauv, ua rau cov txiaj ntsig tsis raug.
Txhawm rau tiv thaiv qhov kev ua yuam kev no, nco ntsoov koj lub zog thiab tuav tes ruaj khov thaum lub raj xa dej. Tsis txhob tso siab dhau ntawm lub pipette los yog siv lub zog tsis tsim nyog thaum muab tshuaj los yog aspirating. Tsis tas li ntawd, muab lub taub hau ze rau ntawm cov kua dej yam tsis tau kov lub thawv phab ntsa. Los ntawm kev xyaum ua cov txheej txheem zoo pipetting, koj tuaj yeem txo qhov kev pheej hmoo ntawm cov qauv paug.
5. Cov txheej txheem faib khoom tsis raug
Qhov kawg yuam kev kom tsis txhob yog cov txheej txheem muab tshuaj tsis raug. Kev xa tawm tsis raug tuaj yeem ua rau muaj qhov tsis sib xws lossis tsis sib xws ntawm cov kua dej, cuam tshuam rau kev siv tau ntawm koj cov txiaj ntsig kev sim. Feem ntau yuam kev suav nrog kev xa tawm sai lossis tsis tswj tau, poob dej, lossis ua yuam kev tawm ntawm cov seem seem hauv qhov taub.
Txhawm rau xyuas kom meej thiab zoo ib yam, xyuam xim rau qhov ceev thiab lub kaum sab xis ntawm lub pipette thaum ua haujlwm. Ua kom tswj tau thiab ruaj khov ceev, cia cov kua dej ntws zoo. Tom qab muab tshuaj, tos ib lub sijhawm luv luv kom tso cai rau cov kua dej ntxiv kom ntws tawm ua ntej tshem cov pipette los ntawm lub thawv.
zam kev ua yuam kev thaum siv cov lus qhia pipette hauv chav kuaj yog qhov tseem ceeb kom tau txais txiaj ntsig zoo thiab rov tsim dua tshiab. Los ntawm kev xaiv qhov tseeb pipette taub hau, muab txuas kom zoo, xyaum cov txheej txheem pipetting kom raug, tiv thaiv cov qauv sib kis, thiab siv cov txheej txheem xa tawm kom raug, koj tuaj yeem txhim kho qhov tseeb thiab qhov tseeb ntawm koj qhov kev sim.
Post lub sij hawm: Mar-06-2024