he aha ka maʻi in vitro?

ʻO nā diagnostics in vitro e pili ana i ke kaʻina hana o ka ʻike ʻana i kahi maʻi a i ʻole ke kūlana ma o ka hoʻokaʻawale ʻana i nā laʻana olaola mai waho o ke kino. Ke hilinaʻi nui nei kēia kaʻina hana i nā ʻano molecular biology like ʻole, me ka PCR a me ka unuhi ʻana o ka nucleic acid. Eia kekahi, ʻo ka mālama ʻana i ka wai he mea nui o ka diagnostics in vitro.

ʻO ka PCR a i ʻole ka polymerase chain reaction kahi hana i hoʻohana ʻia e hoʻonui i nā ʻāpana kikoʻī o DNA. Ma ka hoʻohana ʻana i nā primers kikoʻī, ʻae ka PCR i ka hoʻonui koho ʻana i nā kaʻina DNA, a laila hiki ke nānā ʻia no nā hōʻailona o ka maʻi a i ʻole ka maʻi. Hoʻohana pinepine ʻia ka PCR e ʻike i nā maʻi viral, bacterial, fungal a me nā maʻi parasitic, a me nā maʻi genetic a me ka maʻi kanesa.

ʻO ka unuhi ʻana o ka waikawa nucleic kahi ʻano hana e hoʻokaʻawale a hoʻomaʻemaʻe i ka DNA a i ʻole RNA mai nā laʻana olaola. Loaʻa nā ʻakika nucleic i unuhi ʻia no ka nānā hou ʻana, me ka PCR. He mea nui ka lawe ʻana i ka waikawa nucleic no ka hōʻoia pololei a me ka hoʻolālā ʻana i ka mālama ʻana no nā maʻi like ʻole a me nā kūlana.

ʻO ka lawelawe ʻana i ka wai he kaʻina e pili ana i ka hoʻoili pololei ʻana, ka hāʻawi ʻana a me ka hui ʻana o nā liʻiliʻi liʻiliʻi o nā wai i loko o kahi hoʻonohonoho hoʻokolohua. Ua kaulana nā ʻōnaehana hoʻokele wai i nā makahiki i hala iho nei no ka mea e hiki ai iā lākou ke kiʻekiʻe o ka throughput a me ka pololei o ka hoʻāʻo ʻana e like me ka PCR a me ka ʻokiʻoki nucleic acid.

Ke hilinaʻi nui nei nā diagnostics in vitro i kēia mau ʻenehana biology molecular no ka mea e ʻae lākou i ka ʻike ʻana a me ka nānā ʻana i nā maʻi e pili ana i ka maʻi a me ka molecular markers. No ka laʻana, hiki ke hoʻohana ʻia ka PCR no ka hoʻonui ʻana i nā kaʻina gene kikoʻī e pili ana i ka maʻi kanesa o ka umauma, ʻoiai hiki ke hoʻohana ʻia ka unuhi ʻana o ka nucleic acid no ka hoʻokaʻawale ʻana i ka DNA i loaʻa i ka maʻi maʻi mai nā laʻana koko.

Ma kahi o kēia mau ʻenehana, hoʻohana ʻia nā ʻano hana like ʻole a me nā mea hana i loko o ka diagnostics in vitro. No ka laʻana, hoʻohana nui ʻia nā mea microfluidic i nā noi kiʻekiʻe-throughput a me ka point-of-care. Hoʻolālā ʻia kēia mau mea hana no ka mālama pono ʻana a me ka hoʻohana ʻana i nā liʻiliʻi liʻiliʻi o nā wai, e hoʻolilo iā lākou i kūpono no ka PCR a me nā noi biology molecular ʻē aʻe.

Pēlā nō, ke pāʻani nei nā ʻenehana hou aʻe (NGS) i kahi hana koʻikoʻi i ka diagnostics in vitro. Hiki i ka NGS ke kaʻina like o nā miliona o nā ʻāpana DNA, hiki ke ʻike wikiwiki a pololei hoʻi i nā hoʻololi genetic pili i ka maʻi. Hiki i ka NGS ke hoʻololi i ka hōʻailona a me ka mālama ʻana i nā maʻi genetic a me ka maʻi kanesa.

I ka hōʻuluʻulu manaʻo, he ʻāpana koʻikoʻi nā diagnostics in vitro o ka lāʻau lapaʻau hou a hilinaʻi nui i nā ʻenehana biology molecular e like me PCR, nucleic acid extraction, a me ka mālama wai. ʻO kēia mau ʻenehana, me nā ʻenehana e like me nā microfluidic device a me NGS, ke hoʻololi nei i ke ʻano o kā mākou ʻike a mālama ʻana i ka maʻi. Ke hoʻomau nei ka ʻenehana i ka holomua, ʻoi aku ka pololei a me ka maikaʻi o nā diagnostics in vitro, i ka hopena e hoʻomaikaʻi i nā hopena maʻi a me ka maikaʻi o ke ola.

At Suzhou Ace Biomedical,ua hoʻolaʻa mākou i ka hāʻawi ʻana iā ʻoe i nā lako lab kiʻekiʻe loa no kāu mau pono ʻepekema āpau. ʻO kā mākou ʻano o nā ʻōlelo aʻoaʻo pipette, nā pā PCR, nā paipu PCR, a me ke kiʻi hoʻopaʻa paʻa i hoʻolālā ʻia a hana ʻia e hōʻoia i ka pololei a me ka pololei i kāu mau hoʻokolohua āpau. Ua kūpono kā mākou ʻōlelo aʻoaʻo pipette me nā ʻano nui o nā pipettes a hele mai i nā ʻano nui like ʻole e kūpono i kāu mau pono kikoʻī. Hana ʻia kā mākou mau papa PCR a me nā paipu mai nā mea kiʻekiʻe loa a ua hoʻolālā ʻia e kū i nā pōʻai wela me ka mālama ʻana i ka pono o ka laʻana. Hāʻawi kā mākou kiʻi sealing i kahi sila paʻa e pale ai i ka evaporation a me ka haumia mai nā mea waho. Hoʻomaopopo mākou i ke koʻikoʻi o nā lako lab pono a kūpono hoʻi, ʻo ia ke kumu e hoʻoikaika ai mākou e hāʻawi iā ʻoe i nā huahana a me nā lawelawe maikaʻi loa. Loaʻa mau kā mākou hui loea e kōkua iā ʻoe me nā nīnau a i ʻole nā ​​​​pilikia āu e loaʻa ai.