He aha ka pā PCR?

ʻO ka pā PCR he ʻano o ka primer, dNTP, Taq DNA polymerase, Mg, template nucleic acid, buffer a me nā mea lawe ʻē aʻe i komo i ka hoʻonui ʻana i ka Polymerase Chain Reaction (PCR).

1. Hoʻohana i ka pā PCR

Hoʻohana nui ʻia ia ma nā ʻano genetics, biochemistry, immunity, lāʻau lapaʻau, a me nā mea ʻē aʻe, ʻaʻole wale i ka noiʻi kumu e like me ka hoʻokaʻawale ʻana o ka gene, cloning a me ka nucleic acid sequence analysis, akā i ka hōʻoia ʻana o nā maʻi a i ʻole kahi wahi i loaʻa ai ka DNA. a me RNA. He mea hoʻohana i hoʻokahi manawa i ka hale hana. Huahana.

2.96 Well PCRMea papa

ʻO kāna mea ponoʻī he polypropylene (PP) i kēia mau lā, no laila hiki iā ia ke hoʻololi maikaʻi i nā hoʻonohonoho wela kiʻekiʻe a haʻahaʻa i ka PCR reaction process, a hiki ke hoʻokō i ka wela kiʻekiʻe a me ka sterilization kiʻekiʻe. I mea e hoʻokō ai i ka hana kiʻekiʻe-throughput i hui pū me kahi pū lālani, mīkini PCR, a me nā mea ʻē aʻe, ʻoi aku ka maʻamau o nā papa PCR 96-well a i ʻole 384-well. Kūlike ke ʻano o ka pā i ke kūlana honua SBS, a no ka hoʻololi ʻana i nā mīkini PCR o nā mea hana like ʻole, hiki ke hoʻokaʻawale ʻia i ʻehā mau ʻano hoʻolālā: ʻaʻohe kapa, ʻaʻohe hapa, ke kapa kiʻekiʻe a me ke kapa piha e like me ka hoʻolālā ʻana.

3. ʻO ke kala nui o ka pā PCR

ʻO nā mea maʻamau he akaka a keʻokeʻo, ma waena o nā papa PCR keʻokeʻo i kūpono no ka PCR quantitative fluorescent hou.