He aha nā Cryovial?
ʻO nā hue mālama mālama cryogeniche mau ipu liʻiliʻi, uhi ʻia a cylindrical i hoʻolālā ʻia no ka mālama ʻana a me ka mālama ʻana i nā laʻana ma nā wela haʻahaʻa loa. ʻOiai ua hana ʻia kēia mau hue mai ke aniani, i kēia manawa ua hana pinepine ʻia lākou mai ka polypropylene no ka maʻalahi a me nā kumu kumu kūʻai. Ua hoʻolālā maikaʻi ʻia nā Cryovial e pale i nā mahana haʻahaʻa e like me ka -196 ℃, a no ka hoʻokipa ʻana i nā ʻano kelepona like ʻole. ʻOkoʻa kēia mau mea mai nā cell stem diagnostic, microorganisms, cell primary a hiki i nā laina kelepona paʻa. Ma waho aʻe o kēlā, aia kekahi mau mea ola multicellular liʻiliʻi i mālama ʻia i lokonā hue mālama mālama cryogenic, a me ka waika nucleic a me nā protein e pono e mālama ʻia ma nā pae mahana mālama cryogenic.
Hiki mai nā hue mālama Cryogenic i nā ʻano ʻano like ʻole, a ʻo ka loaʻa ʻana o ke ʻano kūpono e hoʻokō ai i kāu mau pono āpau e hōʻoiaʻiʻo ʻoe e mālama pono i ka hōʻailona me ka ʻole o ka uku. E heluhelu i kā mākou ʻatikala e aʻo hou e pili ana i nā kumu kūʻai kūʻai nui i ke koho ʻana i ka cryovial kūpono no kāu noi ʻoihana.
ʻO nā waiwai o ka Vial Cryogenic e noʻonoʻo ai
Nā Loko Kūwaho vs
Hana pinepine ka poʻe i kēia koho ma muli o ka makemake pilikino, akā aia nō nā ʻokoʻa hana koʻikoʻi e noʻonoʻo ai ma waena o nā ʻano ʻelua o ke kaula.
He nui nā laboratories e koho pinepine i nā hue i hoʻopaʻa ʻia i loko e hōʻemi i ka nui o ka waiho ʻana o ka paipu e hiki ai ke komo i loko o nā pahu pahu hau. ʻOiai ʻo kēia, hiki paha iā ʻoe ke noʻonoʻo ʻo ke koho i hoʻopaʻa ʻia i waho ke koho maikaʻi loa no ʻoe. Manaʻo ʻia lākou e lawe i kahi pōʻino haʻahaʻa haʻahaʻa, ma muli o ka hoʻolālā e ʻoi aku ka paʻakikī o nā mea ʻē aʻe ma mua o ka laʻana e komo i ka hue.
ʻO nā hue i hoʻopaʻa ʻia i waho e makemake nui ʻia no nā noi genomic, akā manaʻo ʻia kēlā me kēia koho i kūpono no ka biobanking a me nā noi hoʻonui kiʻekiʻe.
Hoʻokahi mea hope e noʻonoʻo ai i ka hoʻopaʻa ʻana - inā hoʻohana kāu kikowaena i ka automation, pono paha ʻoe e noʻonoʻo i ke ʻano o ke kaula e hoʻohana ʻia me nā mea hoʻopaʻa lima.
Volume Waihona
Loaʻa nā hue cryogenic i nā ʻano nui like ʻole e uhi i ka hapa nui o nā pono, akā ʻo ka hapa nui ma waena o ka hiki o 1 mL a me 5 mL.
ʻO ke kī, ʻo ia ka hōʻoia ʻaʻole i hoʻopiha piha ʻia kāu cryovial a loaʻa kahi lumi ʻē aʻe, inā e pehu ka hāpana i ka wā hau. Ma ka hoʻomaʻamaʻa, ʻo ia hoʻi, koho nā laboratories no 1 mL vials i ka mālama ʻana i nā laʻana o 0.5 mL o nā cell i hoʻokuʻu ʻia i cryoprotectant, a me 2.0 mL vials no 1.0 mL o ka hāpana. ʻO kekahi ʻōlelo aʻoaʻo no ka hoʻopiha ʻole ʻana i kāu hue, ʻo ia ka hoʻohana ʻana i nā cryovials me nā māka puka, e hōʻoia ʻoe e pale aku i ka pehu ʻana i hiki ke hoʻoheheʻe ʻia.
Kāpena Screw vs Flip Top
ʻO ke ʻano o ka luna āu e koho ai e hilinaʻi nui ʻia inā ʻoe e hoʻohana ana i ka wai nitrogen a i ʻole. Inā ʻoe, a laila pono ʻoe i nā cryovials i uhi ʻia. E hōʻoia kēia ʻaʻole hiki iā lākou ke pahū me ka pōʻino ma muli o ka lawelawe hewa ʻole a i ʻole nā hoʻololi wela. Hoʻohui ʻia, ʻae nā pāpale wili no ka hoʻihoʻi maʻalahi mai nā pahu cryogenic a ʻoi aku ka maikaʻi o ka mālama ʻana.
Eia nō naʻe, inā ʻaʻole ʻoe e hoʻohana ana i ka nitrogen pae wai a makemake ʻoe i kahi ʻoi aku ka maʻalahi o ka wehe ʻana, a laila ʻoi aku ka maikaʻi o ka flip top. Mālama kēia iā ʻoe i ka manawa maʻalahi no ka wehe ʻana, a he mea pono loa ia i nā hana throughput kiʻekiʻe a me nā mea e hoʻohana ana i nā kaʻina hana.
Palekana Sila
ʻO ke ala maikaʻi loa e hōʻoia ai i kahi sila paʻa, ʻo ia ka hōʻoia ʻana ua hana ʻia kāu pāpale cryovial a me ka ʻōmole mai ka mea like. E hōʻoia kēia e emi a hoʻonui like lākou. Inā hana ʻia lākou me ka hoʻohana ʻana i nā mea like ʻole, a laila e emi a hoʻonui lākou i nā ʻano like ʻole e like me ka hoʻololi ʻana o ka mahana, alakaʻi i nā āpau a me ka leakage a me ka hopena.
Hāʻawi kekahi mau ʻoihana i nā mea holoi ʻelua a me nā flange no ka pae kiʻekiʻe o ka palekana laʻana ma nā cryovial wili waho. Manaʻo ʻia nā cryovial O-Ring i mea hilinaʻi loa no nā cryovial wili i loko.
Aniani vs
No ka palekana a me ka ʻoluʻolu, nui nā hale hana i kēia manawa e hoʻohana i ka plastic, maʻamau polypropylene, ma kahi o nā ampules aniani hiki ke hoʻopaʻa ʻia. Manaʻo ʻia nā ampules aniani he koho kahiko ʻoiai i ka wā o ke kaʻina hoʻopaʻa ʻana e hiki mai ana nā leaks pinhole i ʻike ʻole ʻia, a ke hoʻoheheʻe ʻia ma hope o ka waiho ʻana i loko o ka wai hau e hoʻohūhū ai. ʻAʻole kūpono lākou i nā ʻenehana lepili hou, ʻo ia ke kī i ka hōʻoia ʻana i ka traceability sample.
Kūʻokoʻa vs Poepoe lalo
Loaʻa nā kīʻaha cryogenic ma ke ʻano he kū ponoʻī me nā ʻano hōkū, a i ʻole ma ke ʻano he poepoe. Inā pono ʻoe e kau i kāu mau hue ma luna o ka ʻili a laila e koho pono i ke kū ponoʻī
Hiki i ka ʻimi ʻana a me ka Laʻana
ʻAʻole ʻike pinepine ʻia kēia wahi o ka mālama cryogenic akā he mea koʻikoʻi ka nānā ʻana i ka laʻana a me ka traceability. Hiki ke mālama ʻia nā laʻana cryogenic no nā makahiki he nui, a ma luna o ka manawa e hiki ai i nā limahana ke hoʻololi a me ka mālama ʻole ʻana i nā moʻolelo hiki ke ʻike ʻole ʻia.
E hōʻoia e koho i nā hue e hoʻomaʻamaʻa maʻalahi i ka ʻike ʻana. ʻO nā mea āu e nānā ai e komo pū:
ʻO nā wahi kākau nui e hoʻopaʻa i nā kikoʻī kūpono i hiki ke loaʻa nā moʻolelo inā loaʻa kahi hue ma kahi kūpono ʻole - maʻamau ka ʻike kelepona, ka lā maloʻo, a me nā hua mua o ka mea kuleana ua lawa.
Nā barcode e kōkua i ka hoʻokele hoʻohālike a me nā ʻōnaehana hoʻopaʻa
Nā pāpale kala
He memo no ka wā e hiki mai ana - ke kūkulu ʻia nei nā ʻāpana ultra-cold-resistant, i ka wā i hoʻokomo ʻia i loko o nā cryovial pākahi, hiki ke mālama i kahi mōʻaukala wela a me nā ʻike kikoʻī o ka pūʻulu, nā hopena hoʻāʻo a me nā palapala maikaʻi e pili ana.
Ma waho aʻe o ka noʻonoʻo ʻana i nā kikoʻī like ʻole o nā hue i loaʻa, pono e hāʻawi ʻia kekahi manaʻo i ke kaʻina hana ʻenehana o ka mālama ʻana i nā cryovial i loko o ka nitrogen wai.
Ka Mahana Waihona
Nui nā ala mālama no ka mālama ʻana i ka cryogenic o nā laʻana, e hana ana kēlā me kēia ma kahi mahana kikoʻī. ʻO nā koho a me ka mahana a lākou e hana ai:
Māhele wai LN2: mālama i kahi mahana o -196 ℃
Vapor phase LN2: hiki ke hana ma nā pae wela kūikawā ma waena o -135°C a me -190°C ma muli o ke kumu hoʻohālike.
ʻO nā pahu hau hau Nitrogen: -20°C a i -150°C
ʻO ke ʻano o nā pūnaewele e mālama ʻia ana a me ke ʻano o ka mālama ʻana i makemake ʻia e ka mea noiʻi e hoʻoholo i kahi o nā koho ʻekolu i loaʻa i kāu keʻena hana.
Eia nō naʻe, ma muli o ka haʻahaʻa haʻahaʻa loa i hoʻohana ʻia ʻaʻole kūpono a palekana paha nā paipu a pau. Hiki i nā mea waiwai ke lilo i mea ma'alahi ma nā wela ha'aha'a loa, me ka ho'ohana 'ana i ka hue kūpono 'ole no ka ho'ohana 'ana i ka wela i koho 'ia e naha a pohā paha ka moku i ka wā e waiho ai a ho'olulu.
E nānā pono i nā ʻōlelo aʻoaʻo a nā mea hana no ka hoʻohana pono ʻana no ka mea ua kūpono kekahi mau hue wai cryogenic no nā mahana haʻahaʻa e like me -175°C, ʻo kekahi -150°C ʻē aʻe he 80°C wale nō.
He mea kūpono hoʻi e hoʻomaopopo i ka nui o nā mea hana e ʻōlelo nei ʻaʻole kūpono kā lākou kīʻaha cryogenic no ka hoʻokomo ʻana i ka pae wai. Inā mālama ʻia kēia mau hue i loko o ka wai i ka wā e hoʻi ai i ka lumi wela e haki paha kēia mau hue a i ʻole ko lākou mau sila pāpale ma muli o ke kūkulu wikiwiki ʻana o ke kaomi ma muli o nā liʻiliʻi liʻiliʻi.
Inā mālama ʻia nā cell i loko o ka ʻāpana wai o ka naikokene wai, e noʻonoʻo e mālama i nā keena i loko o nā hue cryogenic kūpono i hoʻopaʻa ʻia i ka wela i loko o nā paipu cryoflex a i ʻole e mālama i nā cell i loko o nā ampules aniani i pani ʻia.
Ka manawa hoʻouna: Nov-25-2022