Pipette ɗaya ne daga cikin kayan aikin gama gari da ake amfani da su a cikin dakunan gwaje-gwaje na ilmin halitta, na asibiti, da na nazari inda ake buƙatar auna ruwa daidai da canja wurin lokacin yin dilution, tantancewa ko gwajin jini. Akwai su kamar:
① tashoshi ɗaya ko tashoshi da yawa
② ƙayyadaddun ƙara ko daidaitacce
③ manual ko lantarki
Menene Pipettes Single-Channel?
Pipette tashoshi ɗaya yana ba masu amfani damar canja wurin aliquot guda ɗaya a lokaci guda. Ana amfani da waɗannan a cikin dakunan gwaje-gwaje tare da ƙananan samfurori na samfurori, wanda sau da yawa zai iya zama wadanda ke da hannu wajen bincike da ci gaba.
Pipette mai tashar tashoshi ɗaya yana da kai guda ɗaya don nema ko rarraba daidaitattun matakan ruwa ta hanyar zubarwa.tip. Ana iya amfani da su don aikace-aikace da yawa a cikin dakunan gwaje-gwaje waɗanda ke da ƙaramin abin sarrafawa. Wannan sau da yawa dakunan gwaje-gwaje ne waɗanda ke yin bincike mai alaƙa da sinadarai na nazari, al'adar tantanin halitta, kwayoyin halitta ko rigakafi.
Menene Pipettes Multi-Channel?
Multi-tashar pipettes suna aiki daidai da pipettes tashoshi ɗaya, amma suna amfani da yawatukwicidon aunawa da rarraba daidai adadin ruwa lokaci guda. Saitunan gama gari sune tashoshi 8 ko 12 amma ana samun saitin tashoshi 4, 6, 16 da 48. Hakanan ana iya siyan nau'ikan benci na tashoshi 96.
Yin amfani da pipette na tashoshi da yawa, yana da sauƙin cika rijiyar 96-, 384-, ko 1,536 da sauri.farantin microtiter, wanda zai iya ƙunsar samfurori don aikace-aikace irin su haɓaka DNA, ELISA (gwajin bincike), nazarin motsa jiki da kuma nazarin kwayoyin halitta.
Single-Channel vs. Multi-Channel Pipettes
inganci
Pipette guda ɗaya tashoshi yana da kyau lokacin yin aikin gwaji. Wannan saboda galibi ya ƙunshi yin amfani da bututu ɗaya ne kawai, ko wasan giciye guda ɗaya don yin ƙarin jini.
Koyaya, wannan da sauri ya zama kayan aiki mara inganci lokacin da aka haɓaka kayan aiki. Lokacin da akwai samfura da yawa/reagents don canjawa wuri, ko ana gudanar da gwaje-gwaje mafi girma a ciki96 rijiyar microtitre faranti, akwai hanya mafi inganci don canja wurin ruwa sannan ta amfani da pipette mai tashar tashoshi ɗaya. Ta hanyar amfani da pipette mai yawan tashoshi a maimakon haka, yawan matakan bututun yana raguwa sosai.
Teburin da ke ƙasa yana nuna adadin matakan bututun da ake buƙata don tashar tashoshi ɗaya, saitin tashar tashoshi 8 da 12.
Adadin matakan bututun da ake buƙata (6 reagents x96 To Microtitre Plate)
Pipette guda ɗaya tashoshi: 576
8-Channel Pipette: 72
12-Channel Pipette: 48
Girman Bututu
Babban bambanci tsakanin pipettes guda ɗaya da tashoshi masu yawa shine ƙarar kowace rijiyar da za a iya canjawa wuri ɗaya lokaci ɗaya. Ko da yake ya dogara da samfurin da ake amfani da shi, gabaɗaya ba za ku iya canja wurin girman girman kowane kai akan pipette tashoshi da yawa ba.
Ƙimar pipette mai tashar tashoshi ɗaya na iya canja wurin jeri tsakanin 0.1ul da 10,000ul, inda kewayon pipette mai yawan tashoshi yana tsakanin 0.2 da 1200ul.
Samfurin Loading
A tarihi, pipettes na tashoshi da yawa sun kasance marasa ƙarfi kuma suna da wahalar amfani. Wannan ya haifar da lodin samfurin da bai dace ba, tare da matsalolin lodawatukwici. Akwai sabbin samfura da ake samu yanzu duk da haka, waɗanda suka fi dacewa da masu amfani kuma suna tafiya wasu hanyoyi don magance waɗannan batutuwa. Har ila yau, ya kamata a lura da cewa ko da yake lodin ruwa na iya zama ɗan ƙaramin kuskure tare da pipette na tashar tashoshi da yawa, sun fi dacewa su zama mafi daidai gaba ɗaya fiye da tashoshi guda ɗaya saboda rashin kuskuren da ke faruwa daga kuskuren mai amfani a sakamakon gajiya. duba sakin layi na gaba).
Rage Kuskuren Dan Adam
Yiwuwar kuskuren ɗan adam yana raguwa sosai yayin da adadin matakan bututun ya ragu. Ana cire sauye-sauye daga gajiya da rashin jin daɗi, yana haifar da bayanai da sakamakon da ke dogara da sake sakewa.
Daidaitawa
Don tabbatar da daidaito da daidaiton na'urorin sarrafa ruwa, ana buƙatar daidaitawa na yau da kullun. Standard ISO8655 ya ce kowane tashoshi dole ne a gwada shi kuma a ba da rahoto. Yawancin tashoshi da pipette ke da shi, yana ɗaukar tsawon lokaci don daidaitawa wanda zai iya ɗaukar lokaci.
Bisa ga pipettecalibration.net daidaitaccen daidaitawar 2.2 akan pipette mai tashar tashoshi 12 yana buƙatar hawan pipetting 48 da ma'aunin nauyi (2 kundin x 2 maimaita x 12 tashoshi). Dangane da saurin mai aiki, wannan na iya ɗaukar sama da awanni 1.5 akan kowane pipette. Dakunan gwaje-gwaje a Burtaniya da ke buƙatar daidaitawar UKAS na buƙatar yin jimillar ma'auni na gravimetric 360 (yawan 3 x 10 maimaita x 12 tashoshi). Yin wannan adadin gwaje-gwajen da hannu ya zama maras amfani kuma yana iya fin karfin tanadin lokacin da aka samu ta amfani da pipette mai yawan tashoshi a wasu labs.
Koyaya, don shawo kan waɗannan matsalolin ana samun sabis na calibration na pipette daga kamfanoni da yawa. Misalan waɗannan sune Gilson Labs, ThermoFisher da Pipette Lab.
Gyara
Ba wani abu bane da mutane da yawa suke tunani akai lokacin siyan sabon pipette, amma nau'ikan pipettes na tashoshi da yawa ba za a iya gyara su ba. Wannan yana nufin idan tashar 1 ta lalace, ana iya maye gurbin gabaɗayan manifold. Duk da haka, wasu masana'antun suna sayar da masu maye gurbin tashoshi ɗaya, don haka tabbatar da duba gyara tare da masana'anta lokacin siyan pipette mai yawan tashoshi.
Takaitawa - Single vs Multi-Channel Pipettes
Pipette mai tashar tashoshi da yawa kayan aiki ne mai mahimmanci ga kowane dakin gwaje-gwaje wanda ke da wani abu fiye da ƙaramin kayan samfuri. A kusan kowane yanayi madaidaicin ƙarar ruwan da ake buƙata don canja wuri yana tsakanin iyawar kowanetipakan pipette mai tashar tashoshi da yawa, kuma akwai ƙananan kurakurai masu alaƙa da wannan. Duk wani ƙarami mai ƙarfi a cikin yin amfani da pipette mai tashar tashoshi da yawa ya fi nauyi sosai ta raguwar yawan aiki, wanda aka samu ta hanyar rage yawan matakan bututun. Duk wannan yana nufin ingantaccen ta'aziyyar mai amfani, da rage kuskuren mai amfani.
Lokacin aikawa: Dec-16-2022