CryovialsAna amfani da amfani da shi don adana kirayen cryobenic na layin tantanin halitta da sauran mahimman kayan halittu, a cikin lalata da aka cika da nitrogen ruwa.

Akwai matakai da yawa da hannu a cikin nasarar adana sel a cikin ruwa nitrogen. Yayin da ka'idar manufa ce mai saurin daskarewa, ainihin aikin da aka yi amfani da shi ya dogara da nau'in tantanin halitta da kuma cryoprote wanda aka yi amfani da shi. Akwai la'akari da aminci da yawa kuma mafi kyawun ayyukan da za a yi la'akari da su lokacin da aka adana sel a irin wannan yanayin zafi.

Wannan post din da nufin bayar da taƙaitaccen hoto na yadda ake adana cryovials a cikin nitrogen ruwa.

Menene masu kukan

Cryovials ƙanana ne, vials vials da aka tsara don adana samfuran ruwa a matsanancin yanayin zafi. Sun tabbatar da cewa sel aka kiyaye shi a cikin kukan cryoprotectant ba su shiga kai tsaye tare da ruwa nitrogen ba, rage haɗarin kararwar salula alhali har yanzu yana amfana da tasirin sanyi na ruwa mai ruwa.

Ana samun vials yawanci a cikin kewayon kundin da zane-zane - suna iya zama a cikin ƙasa ko na waje da lebur ko zagaye. Sertile da kuma ba a wadatar da kayan talla ba.

Wanda ya yi amfani da shiCyrovialsDon adana sel a cikin ruwa nitrogen

Rango na NHS da masu dakikarwa masu zaman kansu, da kuma cibiyoyin bincike sun kware a cikin igiyar jini, epitelolic da tushe na ilmin halitta suna amfani da ƙwayoyin tantanin halitta don kuka.

Kwayoyin da aka adana a wannan hanyar sun hada da sel da sel, sel na hematopoetic, sel na hanji, sel na ciki, sel myboropotent sel.

Takaitaccen bayani game da yadda ake adana kukan ruwa a cikin ruwa nitrogen

Cryperepleepack tsari ne wanda ke kiyaye sel da sauran ginin halittu ta hanyar sanyaya su zuwa yanayin zafi sosai. Kwayoyin za a iya adanar su a cikin ruwa na ruwa na shekaru ba tare da asarar tantanin halitta ba. Wannan lamari ne na hanyoyin aiki.

Shiri na tantanin halitta

Hakikanin hanya don shirya samfurori za su bambanta dangane da nau'in tantanin halitta, amma a gabaɗaya, ana tattara sel kuma centrifughed da centrifuged don haɓaka pel mai arzikin sel. Wannan pellet ɗin an sake shi ne a cikin supernant gauraye da cryoprotectorant ko matsakaita mai kukan.

Matsayi matsakaici

Wannan matsakaiciyar tana aiki don adana sel a cikin yanayin ƙananan ƙananan za a ƙasƙantar da su ta hanyar hana tasirin intra da kuma abubuwan lu'ulu'u da ƙwayoyin cuta. Matsalar su ita ce samar da ingantacciyar muhalli ga sel da kyallen takarda a lokacin daskarewa, ajiya, da thawing tafiyar matakai.

A matsakaici kamar sabo plasma plasma (FFP), ɗakunan ajiya plasmoyte bayani ko magani mai kyauta, an haɗu da mafita-free hanyoyin ruwa kamar Dimethyl Sulphoxide (DMSO) ko Glycerol.

Sample samfurin da aka sake cire pellet ana hade cikin crypropylene cryovials kamarSuzhou Ace Biomeyardial Kamfanin Kamfanin Cryogenic Stans.

Yana da mahimmanci kada ya mamaye cuvials kamar yadda wannan zai ƙara haɗarin fatattaka da yiwuwar sakin abubuwan da ke ciki (1).

Sarrafa daskarewa

Gabaɗaya, jinkirin sarrafa daskarewa yana aiki don nasarar kuka mai nasara.

Bayan samfurori ne da aka sauƙaƙe cikin vialogenic vials, an sanya su a kan kankara kankara ko a cikin 4 ℃ firiji an fara ne a cikin mintuna 5. A matsayin babban jagorar gaba ɗaya, ana sanyaya sel a cikin adadin -1 zuwa -3 a minti (2). An samu wannan ta amfani da mai sanyaya mai sanyaya mai sanyaya ko ta sanya vials a cikin akwatin da aka sanya a cikin akwatin da aka sanya a cikin A -70 ° C sarrafa contraints.

Canja wuri zuwa ruwa nitrogen

Ana canzawa vials mai daskararru mai sanyi zuwa cikin tank mai ruwa don tank mai ruwa don lokacin ba zai dace da zafin jiki na ƙasa da -135 ℃ an kiyaye shi.

Za'a iya samun waɗannan matsanancin yanayin zafi da nutsuwa a cikin ruwa ko tururi mai narkewa.

Ruwa ko tururi?

Adana a cikin ruwa lokaci nitrogen sanannu ne don kula da yanayin sanyi tare da cikakken daidaito, amma yawanci ba a bada shawarar ga dalilai masu zuwa:

• Bukatar manyan kundin (zurfin) na ruwa ruwa wanda haɗari ne. Kona ko maye saboda wannan haɗari ne na gaske.
• DUK CIKIN SAUKI NA FARKO NA FARKO NA FARKO DA ASAPGGILLIS, SHEP B da kuma hoto a matsakaici na nitrogen na ruwa (2,3)
• Yuwuwar ruwa mai ruwa don shiga cikin vials yayin nutsewa. Lokacin da aka cire daga ajiya da kuma warmed zuwa zazzabi a ɗakin, nitrogen hanzari ya faɗi. A sakamakon haka, m na iya farfadowa da aka cire shi lokacin da aka cire daga ajiya na ruwa, ƙirƙirar haɗari daga tarkace mai tashi da tarkace zuwa abubuwan da ke cikin (1, 4).

Saboda waɗannan dalilai, ajiya mai ƙarancin zafin jiki na ƙarancin zafin jiki ya fi yawanci a cikin lokaci na tururi. A lokacin da dole ne a adana samfurori a cikin lokacin ruwa, ya kamata a yi amfani da tubalin ƙwayoyin cuta na musamman.

Downside Palin tururi shi ne cewa babban zafin jiki na tsaye na iya faruwa wanda ya faru a sakamakon zafin zazzabi tsakanin -135 ℃ da -190 ℃. Wannan na wajabta hankali da kulawa da matakan ruwa na ruwa da bambancin zazzabi (5).

Yawancin masana'antun waɗanda ke ba da shawarar cewa Cryovials sun dace don adana ƙasa zuwa -135 ℃ ko don amfani a cikin tururi kawai.

Thawing kwayoyin kukan

Tsarin Thawing yana da damuwa ga al'adun mai sanyi, kuma ana buƙatar dacewa da dabara don tabbatar da ingantaccen yanayi mai kyau, maido, da aikin sel. Daidaitaccen jigon narkewa zai dogara ne akan takamaiman nau'ikan tantanin halitta. Koyaya, saurin hanzari ana ɗaukar matsayin:

• Rage kowane tasiri a jikin wayar salula
• Taimaka rage lokacin fallasa zuwa ga masu siyar da shi a cikin kafofin watsa labarai na daskarewa
• Rage duk wani lalacewa ta hanyar recrystallation

Ruwa na ruwa, wanka na gida, ko kayan aikin sarrafa kansa ana amfani dasu don narke samfuran narkewa.

Mafi yawan lokuta 1 layin sel ya narke a lokaci na tsawon mintina 1-2, a hankali swirling a cikin wani lokacin ruwa na 37% har sai an wanke ɗan ƙaramin kankara a cikin matsakaici na ci gaba.

Ga wasu sel kamar dabbobi masu shayarwa, jinkirin dumama yana da mahimmanci don rayuwarsu.

Yanzu an shirya sel a yanzu don al'adun tantanin halitta, tantanin halitta, ko a game da sel na karuwa.

Ainihi ne na al'ada don ɗaukar ƙananan ranakun samfuran da kuka fi so don yin ƙididdigar tantancewa don ƙwararrun taro don shirya cikin al'ada. Daga nan zaka iya tantance sakamakon hanyoyin mallakar tantanin halitta da kuma tantance hanyar tantanin halitta.

Mafi kyawun ayyukan don adana cryovials

Mai nasarar Cryopare na samfuran da aka adana a cikin abubuwan da suka shafi abubuwa da yawa ciki har da ajiya mai dacewa da rikodin rikodin.

• Tsage sel tsakanin wuraren ajiya- Idan ya ba da izinin, rarrabe sel a tsakanin vials kuma adana su cikin daban wuri don rage haɗarin asarar samfurin saboda kayan aiki.
• Hana gurbatawa- Fita don amfani da vials mai amfani da kayan kwalliya ko autoclave kafin amfani mai zuwa
• Yi amfani da vials daidai don sel- Vials sun shigo cikin kundin ya kunshe tsakanin 1 da 5mls. Guji yawan vials don rage haɗarin fashewa.
• Zaɓi vials na ciki ko na waje- Wasu jami'o'i da aka ba da shawarar su ne suka ba da shawarar matakan aminci - suna iya hana gurbata yayin cika ko lokacin da aka adana su a cikin nitrogen ruwa.
• Hana yare- Yi amfani da subes bi-allon da aka gyara a cikin dunƙule-hula ko o-zobba don hana jiƙa da gurbata.
• Yi amfani da Barcoodes na 2D da La'anar VIals- Don tabbatar da rashin ƙarfi, vials tare da manyan rubuce rubuce wuraren rubutu yana ba da damar kowane vial da za a iya amfani da shi. 2D Barcodes na iya taimakawa wajen gudanar da ayyukan ajiya da rakodin rikodi. Kafofin launuka masu launi suna da amfani ga masu sauƙin fahimta.
• Isasshen gyara ajiya- Don tabbatar da sel ba su rasa, tasoshin ajiya ya kamata koyaushe kula da zafin jiki da matakan ruwa na ruwa. Arara ya kamata a haɗa don faɗakar da masu amfani da kurakurai.

Tsaron tsaro

Liquid Nitrogen ya zama al'ada ta gama gari a cikin bincike na zamani amma yana ɗaukar haɗarin mummunan rauni idan aka yi amfani da shi ba daidai ba.

Yakamata ya dace da kayan kariya na mutum (PPE) don rage hadarin sanyi, ƙonewa da sauran abubuwan da suka faru na nitrogen. Ci

• Safar hannu na safar hannu
• Kujera mai laushi
• Tasri cikakke cikakkiyar garkuwa da ke rufe wuya
• Takalma mai rufewa
• Spashroof filastik gaba

Ya kamata a sanya firiji na nitrogen ruwa a cikin yankunan da ke da iska mai santsi don rage haɗarin shan iska - tserewa daga cikin oxygen oxygen. Manyan shagunan girma yakamata su sami ƙarancin ƙararrawa oxygen.

Yin aiki a cikin nau'i-nau'i lokacin aiwatar da ruwa na ruwa mai kyau kuma ana amfani da shi a waje da lokutan aiki na yau da kullun ya kamata a haramta.

Cryovials don tallafawa aikinku

Kamfanin Suzhou Ace biomedial kamfanin yana ba da babban zaɓi na samfuran da suka haɗu da bukatun kukan sel daban-daban. Fayil ya ƙunshi kewayon tubesas da kuma kewayon bakararre cakuda.

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  Kashi	Abu	Ƙarfi	TafiyaLauni	PCS /Jaka	Jaka / Case
  Ashe05-b bl-n	PP	0.5ml	Black, rawaya, shuɗi, ja, shunayya, fari	500	10
  Aiki15-BL-N	PP	1.5ml	Black, rawaya, shuɗi, ja, shunayya, fari	500	10
  Aiki15-BL-NW	PP	1.5ml	Black, rawaya, shuɗi, ja, shunayya, fari	500	10
  Aiki20-BL-N	PP	2.0ml	Black, rawaya, shuɗi, ja, shunayya, fari	500	10