Cryovialsana amfani da su akai-akai don ajiyar cryogenic na layin tantanin halitta da sauran mahimman kayan halitta, a cikin dewars cike da ruwa nitrogen.

Akwai matakai da yawa a cikin nasarar adana ƙwayoyin sel a cikin ruwa nitrogen. Duk da yake ainihin ka'ida ita ce jinkirin daskarewa, ainihin dabarar da ake amfani da ita ta dogara da nau'in tantanin halitta da kuma cryoprotectant da ake amfani da su. Akwai la'akari da aminci da yawa da mafi kyawun ayyuka don yin la'akari lokacin adana ƙwayoyin sel a irin wannan ƙananan yanayin zafi.

Wannan sakon yana nufin ba da taƙaitaccen bayani kan yadda ake adana cryovial a cikin ruwa nitrogen.

Menene Cryovials

Cryovials ƙanana ne, kwalayen da aka ƙera don adana samfuran ruwa a cikin ƙananan yanayin zafi. Suna tabbatar da cewa ƙwayoyin da aka adana a cikin cryoprotectant ba su shiga cikin hulɗa kai tsaye tare da nitrogen na ruwa ba, suna rage haɗarin karyewar salula yayin da suke cin gajiyar matsanancin yanayin sanyaya ruwa na nitrogen.

Filallun yawanci ana samun su a cikin kewayon ƙira da ƙira - ana iya yin su a ciki ko na waje tare da lebur ko zagaye ƙasa. Hakanan ana samun sifofin bakararre da marassa haihuwa.

Wanene Yayi AmfaniCyrovialsDon Ajiye Kwayoyin A cikin Liquid Nitrogen

Yawancin dakunan gwaje-gwaje na NHS da masu zaman kansu, da kuma cibiyoyin bincike da suka ƙware a banki na jini na igiya, ilmin halitta na epithelial cell, ilimin rigakafi da ilimin halittar kwayoyin halitta suna amfani da cryovials don adana ƙwayoyin cuta.

Kwayoyin da aka adana ta wannan hanyar sun haɗa da Kwayoyin B da T, CHO Cells, Hematopoietic Stem da Progenitor Cells, Hybridomas, Intestinal Sel, Macrophages, Mesenchymal Stem da Progenitor Cells, Monocytes, Myeloma, NK Cells da Pluripotent Stem Cells.

Bayanin Yadda Ake Ajiye Cryovials a cikin Liquid Nitrogen

Cryopreservation wani tsari ne da ke adana sel da sauran abubuwan gina jiki ta hanyar sanyaya su zuwa ƙananan yanayin zafi. Ana iya adana ƙwayoyin sel a cikin ruwa nitrogen na shekaru ba tare da asarar iyawar tantanin halitta ba. Wannan siffa ce ta hanyoyin da aka yi amfani da su.

Shiri Cell

Hanyar da ta dace don shirya samfurori za ta bambanta dangane da nau'in tantanin halitta, amma gaba ɗaya, ana tattara ƙwayoyin sel kuma ana sanya su don haɓaka pellet mai arzikin tantanin halitta. Ana sake dawo da wannan pellet a cikin maɗaukakiyar haɗe da cryoprotectant ko matsakaicin cryopreservation.

Matsakaicin Cryopreservation

Ana amfani da wannan matsakaicin don adana sel a cikin ƙananan yanayin zafi da za a yi musu ta hanyar hana samuwar lu'ulu'u na ciki da na waje da kuma mutuwar tantanin halitta. Matsayin su shine samar da amintaccen yanayi mai kariya ga sel da kyallen takarda yayin daskarewa, ajiya, da narkewa.

Matsakaici kamar sabon daskararre plasma (FFP), maganin heparinised plasmalyte ko maganin jini mara amfani, ana haɗe da maganin da ba shi da kayan dabba tare da cryoprotectants kamar dimethyl sulphoxide (DMSO) ko glycerol.

Samfurin da aka sake-liqued pellet an liquoted a cikin polypropylene cryovials kamarSuzhou Ace Biomedical Kamfanin Cryogenic Storage Vials.

Yana da mahimmanci kada a cika cryovials saboda wannan zai kara haɗarin fashewa da yiwuwar sakin abubuwan ciki (1).

Adadin Daskare Mai Sarrafawa

Gabaɗaya, ana amfani da ƙimar daskare a hankali don samun nasarar adana ƙwayoyin sel.

Bayan an sanya samfuran a cikin vials na cryogenic, ana sanya su a kan rigar kankara ko a cikin firiji na 4 ℃ kuma ana fara aikin daskarewa a cikin mintuna 5. A matsayin jagora na gaba ɗaya, ana sanyaya sel a cikin adadin -1 zuwa -3 a cikin minti ɗaya (2). Ana samun wannan ta amfani da na'ura mai sanyaya shirye-shirye ko ta hanyar sanya filaye a cikin akwati da aka keɓe wanda aka sanya a cikin injin daskare ƙimar ƙimar -70°C zuwa –90°C.

Canja wurin Liquid Nitrogen

Ana tura daskararrun kwalabe na cryogenic zuwa tankin ruwa na nitrogen na wani lokaci mara iyaka idan an kiyaye zafin da bai wuce -135 ℃ ba.

Ana iya samun waɗannan ƙananan yanayin zafi ta hanyar nutsewa cikin ruwa ko tururi lokaci nitrogen.

Liquid ko tururi Phase?

Adana a cikin lokacin ruwa nitrogen an san shi don kula da yanayin sanyi tare da cikakkiyar daidaito, amma galibi ba a ba da shawarar ba saboda dalilai masu zuwa:

• Bukatar babban juzu'i (zurfin) na nitrogen na ruwa wanda shine haɗari mai yuwuwa. Konewa ko asphyxiation saboda wannan haɗari ne na gaske.
• Abubuwan da aka tattara na kamuwa da cuta ta hanyar kamuwa da cuta kamar aspergillus, hep B da viral yada ta hanyar matsakaicin ruwa na nitrogen (2,3)
• Yiwuwar ruwa nitrogen ya zubo a cikin gwangwani yayin nutsewa. Lokacin da aka cire daga ajiya kuma dumi zuwa zafin jiki, nitrogen yana faɗaɗa cikin sauri. Saboda haka, vial na iya rugujewa lokacin da aka cire shi daga ma'ajin nitrogen na ruwa, yana haifar da haɗari daga tarkace mai tashi da fallasa abubuwan da ke ciki (1, 4).

Don waɗannan dalilai, ma'auni mai ƙarancin zafin jiki ya fi yawa a cikin yanayin tururi na nitrogen. Lokacin da samfuran dole ne a adana su a cikin lokacin ruwa, yakamata a yi amfani da bututun cryoflex na musamman.

Rashin ƙasa zuwa lokacin tururi shine cewa matakin zafin jiki na tsaye zai iya faruwa wanda ya haifar da canjin yanayin zafi tsakanin -135 ℃ da -190 ℃. Wannan yana buƙatar kulawa da hankali da himma akan matakan nitrogen na ruwa da bambancin zafin jiki (5).

Yawancin masana'antun sun ba da shawarar cewa cryovials sun dace don ajiya har zuwa -135 ℃ ko don amfani a cikin lokacin tururi kawai.

Narke Kwayoyin Cryopreserved Naku

Hanyar narkewa tana da damuwa ga al'adun daskararre, kuma ana buƙatar kulawa da dabara da kyau don tabbatar da ingantaccen aiki, farfadowa, da aiki na sel. Madaidaicin ladabi na narke zai dogara da takamaiman nau'ikan tantanin halitta. Koyaya, ana ɗaukar saurin narkewa a matsayin misali zuwa:

• Rage kowane tasiri akan dawo da salon salula
• Taimaka rage lokacin fallasa ga abubuwan da ke cikin daskarewa
• Rage kowane lalacewa ta hanyar sake recrystallisation kankara

Ana amfani da wankan ruwa, wankan ƙwanƙwasa, ko kayan aiki na musamman don narke samfura.

Mafi yawan lokuta ana narke layin tantanin halitta 1 a lokaci guda na mintuna 1-2, ta hanyar murɗawa a hankali a cikin ruwan wanka na 37 ℃ har sai an sami ɗan ƙaramin ƙanƙara da ya rage a cikin vial kafin a wanke su a cikin wani wuri mai dumama.

Ga wasu sel kamar embryo masu shayarwa, jinkirin ɗumama yana da mahimmanci don rayuwarsu.

Kwayoyin suna shirye yanzu don al'adar tantanin halitta, keɓewar tantanin halitta, ko kuma a cikin yanayin ƙwayoyin sel na haematopoietic - nazarin iyawa don tabbatar da amincin sel masu bayarwa kafin maganin myeloablative.

Al'ada ce ta al'ada don ɗaukar ƙananan ƙididdiga na samfurin da aka riga aka wanke da aka yi amfani da shi don yin ƙidayar tantanin halitta don tantance yawan tantanin halitta don sanyawa a cikin al'ada. Sannan zaku iya tantance sakamakon hanyoyin keɓewar tantanin halitta kuma ku tantance yuwuwar tantanin halitta.

Mafi kyawun Ayyuka don Ajiye Cryovials

Nasarar ajiyar kuɗaɗen samfuran da aka adana a cikin cryovials ya dogara da abubuwa da yawa a cikin ƙa'idar da suka haɗa da ajiya mai kyau da adana rikodin.

• Raba sel tsakanin wuraren ajiya- Idan kundin ya ba da izini, raba sel tsakanin vials kuma adana su a wurare daban-daban don rage haɗarin asarar samfurin saboda gazawar kayan aiki.
• Hana kamuwa da cuta- Zaɓi don amfani guda ɗaya bakararre cryogenic vials ko autoclave kafin amfani na gaba
• Yi amfani da filaye masu girman da suka dace don sel ɗinku- vials zo a cikin kewayon juzu'i tsakanin 1 da 5mls. A guji cika kwalabe don rage haɗarin fashewa.
• Zaɓi ciki ko na waje zaren cryogenic vials- Wasu jami'o'i suna ba da shawarar vials na ciki don matakan tsaro - kuma suna iya hana kamuwa da cuta yayin cikawa ko lokacin da aka adana su a cikin ruwa nitrogen.
• Hana Fitowa- Yi amfani da hatimin allurar da aka ƙera a cikin screw-cap ko O-zoben don hana yaɗuwa da gurɓatawa.
• Yi amfani da 2D barcodes da lakabin filaye- don tabbatar da ganowa, filaye tare da manyan wuraren rubuce-rubuce suna ba da damar kowane kwano don samun isassun lakabi. 2D barcodes na iya taimakawa tare da sarrafa ajiya da rikodi. Ƙaƙƙarfan madaukai masu launi suna da amfani don sauƙin ganewa.
• Isasshen ajiyar ajiya- Don tabbatar da cewa sel ba su ɓace ba, tasoshin ajiya ya kamata su kula da yawan zafin jiki da matakan nitrogen na ruwa. Ya kamata a sanya ƙararrawa don faɗakar da masu amfani da kurakurai.

Kariyar Tsaro

Liquid nitrogen ya zama aikin gama gari a cikin bincike na zamani amma yana ɗaukar haɗarin mummunan rauni idan aka yi amfani da shi ba daidai ba.

Ya kamata a sa kayan kariya na sirri da suka dace (PPE) don rage haɗarin sanyi, konewa da sauran muggan lamura yayin sarrafa nitrogen mai ruwa. Saka

• Cryogenic safar hannu
• Kafar dakin gwaje-gwaje
• Cikakken garkuwar fuska mai jurewa tasiri wanda kuma ke rufe wuya
• Takalmi na rufaffiyar kafa
• Tufafin filastik mai hana ruwa

Yakamata a sanya firji na ruwa na nitrogen a cikin wuraren da ke da iska mai kyau don rage haɗarin asphyxiation - nitrogen da ya tsere yana vaporizes kuma yana kawar da iskar oxygen na yanayi. Manyan shagunan ƙara ya kamata su sami ƙananan tsarin ƙararrawa na iskar oxygen.

Yin aiki tare da nau'i-nau'i lokacin sarrafa nitrogen na ruwa yana da kyau kuma ya kamata a hana amfani da shi a waje da lokutan aiki na yau da kullun.

Cryovials don Taimakawa Gudun Aikinku

Kamfanin Suzhou Ace Biomedical yana ba da zaɓi mai yawa na samfuran da suka dace da buƙatun ku don kiyaye nau'ikan sel daban-daban. Fayil ɗin ya ƙunshi kewayon tubesas da kewayon cryovial mara kyau.

Our cryovials ne:

• Lab Screw Cap 0.5mL 1.5mL 2.0ml Cryovial Cryogenic Vials Conical Bottom Cryotube tare da Gasket

  ● 0.5ml, 1.5ml, 2.0ml ƙayyadaddun, tare da siket ko ba tare da siket ba
  ● Zane-zane na Conical ko na tsaye, bakararre ko mara bakararre duka suna samuwa
  ● An yi bututun hular da aka yi da polypropylene matakin likita
  PP Cryotube Vials za a iya daskarewa akai-akai kuma a narke
  ● Zane-zane na waje na waje zai iya rage yiwuwar kamuwa da cuta a lokacin maganin samfurin.
  ● Cire hular bututun hayaƙi na duniya don amfani
  ● Tubes sun dace da rotors na kowa
  ● Cryogenic tube o-ring tubes dace da daidaitattun 1-inch da 2-inch, 48riji, 81 rijiyar, 96 rijiyar da akwatunan injin daskarewa 100 rijiya
  ● Mai sarrafa atomatik zuwa 121°C kuma mai daskarewa zuwa -86°C

  SASHE NA NO	KYAUTATA	MURYA	CAPLAUNIYA	PCS/BAG	BAGS/CASE
  ACT05-BL-N	PP	0.5ML	Black, Yellow, Blue, Red, Purple, Fari	500	10
  Saukewa: ACT15-BL-N	PP	1.5ML	Black, Yellow, Blue, Red, Purple, Fari	500	10
  ACT15-BL-NW	PP	1.5ML	Black, Yellow, Blue, Red, Purple, Fari	500	10
  ACT20-BL-N	PP	2.0ML	Black, Yellow, Blue, Red, Purple, Fari	500	10