Masu sana'a na dakin gwaje-gwaje na iya ciyar da sa'o'i a kowace rana suna riƙe da micropipette, da kuma inganta aikin pipetting da kuma tabbatar da ingantaccen sakamako shine sau da yawa kalubale. Zabar madaidaicin micropipette don kowane aikace-aikacen da aka ba shi shine mabuɗin nasarar aikin dakin gwaje-gwaje; ba wai kawai tabbatar da aikin kowane gwaji ba, amma har ma yana ƙara yawan aiki.Fahimtar buƙatun bututun aikin bututun yana bawa masu amfani damar zaɓar pipettes daidai da maimaitawa, amma akwai wasu dalilai da yawa waɗanda yakamata a yi la'akari da su don haɓaka sakamakon bututun da kuma tabbatar da nasarar gwaje-gwaje.
Magana mai zurfi, ruwa ya fada cikin manyan nau'o'i uku: ruwa, danko, da maras kyau.Mafi yawan ruwaye suna dogara ne akan ruwa, yin bututun motsi na iska shine zabi na farko ga mutane da yawa.Yayin da yawancin ruwaye suna aiki da kyau tare da wannan nau'in pipette, ya kamata a zaba pipettes volumetric lokacin aiki tare da ruwa mai danko ko maras kyau.Bambance-bambancen da ke tsakanin waɗannan pipette iri-iri ana nuna su daidai a cikin nau'in nau'in nau'in pipette. - don kyakkyawan sakamako.
Matsaloli biyu mafi mahimmanci da suka shafi sakamakon pipetting shine daidaito da daidaito (Figure 2) . Don cimma matsakaicin daidaiton pipetting, daidaito, da aminci, ya kamata a kiyaye ma'auni da yawa. A matsayin ka'idar babban yatsa, mai amfani ya kamata koyaushe ya zaɓi mafi ƙarancin pipette wanda zai iya ɗaukar nauyin canja wurin da ake so. µl pipette, sakamakon zai iya zama mara kyau. Za a iya samun sakamako mafi kyau tare da 300 µl pipettes, yayin da 50 µl pipettes suna ba da sakamako mafi kyau. Bugu da ƙari, ƙarar da aka saita a kan pipettes na gargajiya na gargajiya na iya canzawa a lokacin pipetting saboda juyawar bazata na plunger. Wannan shine dalilin da ya sa wasu masana'antun pipette sun ɓullo da gyare-gyaren gyare-gyare masu mahimmanci don tabbatar da wani canje-canje masu mahimmanci don yin canje-canjen gyare-gyare na piped. yana taimakawa tabbatar da ingantaccen sakamako ta hanyar nuna daidaito da daidaito na pipette.Wannan tsari ya kamata ya zama mai sauƙi ga mai amfani; misali, wasu lantarki pipettes iya saita calibration tunatarwa, ko ajiye calibration tarihi.Ba kawai pipettes yi la'akari.Idan pipette tip ya zama sako-sako da, leaks, ko fadowa kashe, shi zai iya haifar da iri-iri matsaloli.Wannan na kowa matsala a cikin Lab ne sau da yawa lalacewa ta hanyar yin amfani da general-manufa pipette tukwici, wanda sau da yawa bukatar "tapping."Wannan tsari stretches gefen tip zuwa tip ko da leak tip zuwa ko da leak tip zuwa tip zuwa tip ko leak. haifar da tip don fadowa daga pipette gaba ɗaya.Zaɓin micropipette mai inganci da aka tsara tare da takamaiman shawarwari yana tabbatar da haɗin kai mafi aminci, samar da matsayi mafi girma na aminci da sakamako mafi kyau.Bugu da ƙari, wani abu mai sauƙi kamar pipettes masu launi da tukwici na iya taimakawa masu amfani su tabbatar da cewa an zaɓi nasihu masu kyau don pipettes.
A cikin yanayi mai girma, yana da mahimmanci don zama mai inganci kamar yadda zai yiwu yayin da yake kiyaye aminci da daidaito na tsarin pipetting.Akwai hanyoyi da yawa don inganta aikin pipetting, ciki har da amfani da multichannel da / ko lantarki pipettes.Waɗannan kayan aiki masu mahimmanci sukan ba da nau'o'in nau'i daban-daban na pipetting - irin su juyawa pipetting, rarrabawa mai canzawa, ƙaddamarwa, tsari mai mahimmanci, tsarin tsari da ƙarin tsari. irin su maimaita rarrabawa suna da kyau don rarraba nau'i-nau'i masu yawa na ƙarar guda ɗaya ba tare da cika tip ba. Yin amfani da pipettes guda ɗaya don canja wurin samfurori tsakanin nau'i-nau'i daban-daban na labware na iya zama da sauri da sauri da kuma kuskure. (RSI) .Wasu pipettes har ma suna da ikon bambanta tazarar tip a lokacin pipetting, ba da izinin canja wurin layi ɗaya na samfurori da yawa tsakanin nau'ikan labware daban-daban da tsarin, adana lokutan lokaci (Hoto 3).
Ma'aikatan dakin gwaje-gwaje yawanci suna ciyar da sa'o'i a rana pipetting.Wannan na iya haifar da rashin jin daɗi kuma, a cikin lokuta masu tsanani, har ma da hannu ko rauni na hannu.Mafi kyawun shawara don guje wa waɗannan haɗarin haɗari shine don rage adadin lokacin da kuke riƙe pipette zuwa mafi ƙarancin lokaci. Baya ga wannan, masu amfani yakamata su zaɓi micropipette mara nauyi da daidaitaccen ma'auni tare da taro a tsakiyar cibiyar don mafi kyawun kwanciyar hankali da hannun dama, masu amfani da hannun dama sun dace da kwanciyar hankali na hagu. rikitar da ƙira, da daidaita ƙarar a cikin kwanciyar hankali da sauri don guje wa motsi maras buƙata.Haka kuma, tukwici suna da mahimmanci, kamar yadda ɗorawar tip da fitarwa sau da yawa suna buƙatar ƙarin ƙarfi fiye da pipetting kuma akwai yuwuwar haɗarin rauni, musamman a cikin saitunan da ake buƙata.
Lokacin zabar madaidaicin micropipette don aikace-aikacen ku, yana da mahimmanci don duba kowane fanni na aikin ku.Ta hanyar la'akari da pipette, halayensa, nau'in da ƙarar ruwa da ake pipetted, da tukwici da aka yi amfani da su, masana kimiyya na iya ba da garantin daidai, daidai kuma sakamakon abin dogaro yayin kiyaye yawan aiki da rage haɗarin rauni.
A cikin wannan fitowar, ana kimanta dawo da mahimman ƙididdiga ta HPLC-MS ta amfani da yanayin gauraye mai ƙarfi na musayar SPE microplates. Fa'idodin SEC-MALLS a cikin aikace-aikacen biopharmaceutical…
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Lokacin aikawa: Juni-10-2022